Restriction map for
pF1KE0035
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoT22I FseI
HincII HindII HpaI KasI KpnI
MaeII MamI MfeI MluI MroI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SalI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 304 GGCGTC G[GA]^CG[CT]C
AlwI 431 GGATC GGATC(4/5)
AocI 877 CCTGAGG CC^T[ACGT]AGG
ApaI 76 GGGCCC GGGCC^C
AviII 95 TGCGCA TGC^GCA
AvrII 400 CCTAGG C^CTAGG
BalI 8 TGGCCA TGG^CCA
BanI 844 GGTGCC G^G[CT][GA]CC
BlnI 400 CCTAGG C^CTAGG
BsaHI 304 GGCGTC G[GA]^CG[CT]C
BsiEI 176 CGGCCG CG[GA][CT]^CG
BsmAI 593 GAGAC GTCTC(1/5)
BsrBI 32 CCGCTC CCGCTC(-3/-3)
BsrFI 65 GCCGGT [GA]^CCGG[CT]
BstYI 431 GGATCT [GA]^GATC[CT]
Bsu36I 877 CCTGAGG CC^T[ACGT]AGG
Cfr10I 65 GCCGGT [GA]^CCGG[CT]
EagI 176 CGGCCG C^GGCCG
EclXI 176 CGGCCG C^GGCCG
Eco52I 176 CGGCCG C^GGCCG
Eco81I 877 CCTGAGG CC^T[ACGT]AGG
EcoRV 976 GATATC GAT^ATC
FspI 95 TGCGCA TGC^GCA
HaeII 45 GGCGCT [GA]GCGC^[CT]
HgaI 305 GCGTC GACGC(5/10)
Hin1I 304 GGCGTC G[GA]^CG[CT]C
HindIII 639 AAGCTT A^AGCTT
KspI 281 CCGCGG CCGC^GG
MaeIII 718 GTCAC ^GT[ACGT]AC
MflI 431 GGATCT [GA]^GATC[CT]
MluNI 8 TGGCCA TGG^CCA
MscI 8 TGGCCA TGG^CCA
PflMI 295 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 530 GACTC GAGTC(4/5)
PpuMI 107 GGGACCC [GA]G^G[AT]CC[CT]
SacII 281 CCGCGG CCGC^GG
SapI 394 GAAGAGC GCTCTTC(1/4)
ScaI 24 AGTACT AGT^ACT
TfiI 952 GAATC G^A[AT]TC
Tsp45I 718 GTCAC ^GT[GC]AC
Van91I 295 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 551 CCAGCAGGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 431 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 25 GTAC GT^AC
748 GTAC
AlwNI 88 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
296 CAGAGCCTG
AspHI 268 GAGCTC G[AT]GC[AT]^C
322 GAGCTC
BbsI 129 GAAGAC GAAGAC(2/6)
453 GAAGAC
BfaI 401 CTAG C^TAG
691 CTAG
BglI 10 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
238 GCCGAGGAGGC
BpmI 388 CTCCAG CTGGAG(16/14)
833 CTCCAG
BpuAI 129 GAAGAC GAAGAC(2/6)
453 GAAGAC
BseRI 532 CTCCTC GAGGAG(10/8)
241 GAGGAG
BsiHKAI 268 GAGCTC G[AT]GC[AT]^C
322 GAGCTC
BsmFI 107 GGGAC GGGAC(10/14)
224 GGGAC
BstXI 857 CCATTTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
938 CCAAAGCTATGG
DdeI 702 CTTAG C^T[ACGT]AG
878 CTGAG
DraII 75 GGGGCCC [GA]G^G[ACGT]CC[CT]
107 GGGACCC
DsaI 173 CCACGG C^C[GA][CT]GG
281 CCGCGG
EcoO109I 75 GGGGCCC [GA]G^G[ACGT]CC[CT]
107 GGGACCC
EcoT14I 400 CCTAGG C^C[AT][AT]GG
525 CCTTGG
HinfI 530 GACTC G^A[ACGT]TC
952 GAATC
MaeI 401 CTAG C^TAG
691 CTAG
RsaI 25 GTAC GT^AC
748 GTAC
SacI 268 GAGCTC GAGCT^C
322 GAGCTC
SfaNI 474 GATGC GCATC(5/9)
735 GCATC
StyI 400 CCTAGG C^C[AT][AT]GG
525 CCTTGG
Tsp509I 601 AATT ^AATT
805 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BclI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII EaeI EarI
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI TspRI