Restriction map for
pF1KB6355
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbrPI BbvCI
BbvI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TseI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 566 GGCGCC G[GA]^CG[CT]C
AspHI 70 GAGCTC G[AT]GC[AT]^C
BanI 566 GGCGCC G^G[CT][GA]CC
BanII 70 GAGCTC G[GA]GC[CT]^C
BbeI 566 GGCGCC GGCGC^C
BclI 660 TGATCA T^GATCA
BcnI 642 CCGGG CC^[GC]GG
BglII 397 AGATCT A^GATCT
BmyI 70 GAGCTC G[AGT]GC[ACT]^C
BsaHI 566 GGCGCC G[GA]^CG[CT]C
BseRI 67 GAGGAG GAGGAG(10/8)
BsiHKAI 70 GAGCTC G[AT]GC[AT]^C
Bsp1286I 70 GAGCTC G[AGT]GC[ACT]^C
BspHI 723 TCATGA T^CATGA
BspMI 344 GCAGGT ACCTGC(4/8)
BsrDI 196 GCAATG GCAATG(2/0)
BstF5I 135 GGATG GGATG(2/0)
BstNI 93 CCAGG CC^[AT]GG
BstXI 90 CCACCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 397 AGATCT [GA]^GATC[CT]
Cfr13I 100 GGGCC G^G[ACGT]CC
EcoRI 606 GAATTC G^AATTC
EcoRII 93 CCAGG ^CC[AT]GG
EcoT14I 570 CCAAGG C^C[AT][AT]GG
FbaI 660 TGATCA T^GATCA
FokI 135 GGATG GGATG(9/13)
HapII 642 CCGG C^CGG
Hin1I 566 GGCGCC G[GA]^CG[CT]C
HpaII 642 CCGG C^CGG
KasI 566 GGCGCC G^GCGCC
MflI 397 AGATCT [GA]^GATC[CT]
MseI 209 TTAA T^TAA
MslI 739 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 642 CCGG C^CGG
MvaI 93 CCAGG CC^[AT]GG
NarI 566 GGCGCC GG^CGCC
NciI 642 CCGGG CC^[GC]GG
NdeI 464 CATATG CA^TATG
PshAI 455 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 723 TCATGA T^CATGA
SacI 70 GAGCTC GAGCT^C
Sau96I 100 GGGCC G^G[ACGT]CC
SfoI 566 GGCGCC GGC^GCC
StuI 232 AGGCCT AGG^CCT
StyI 570 CCAAGG C^C[AT][AT]GG
Tru9I 209 TTAA T^TAA
Tsp45I 61 GTGAC ^GT[GC]AC
XcmI 300 CCAACATCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 397 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 348 GTAC GT^AC
575 GTAC
AlwI 24 GGATC GGATC(4/5)
495 GGATC
Asp700I 415 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
602 GAATGAATTC
BbsI 712 GAAGAC GAAGAC(2/6)
549 GTCTTC
BfaI 341 CTAG C^TAG
622 CTAG
BpmI 410 CTCCAG CTGGAG(16/14)
732 CTGGAG
BpuAI 712 GAAGAC GAAGAC(2/6)
549 GTCTTC
BsaJI 65 CCGAGG C^C[ACGT][ACGT]GG
570 CCAAGG
BssKI 93 CCAGG ^CC[ACGT]GG
642 CCGGG
CfoI 567 GCGC GCG^C
783 GCGC
EarI 405 CTCTTC CTCTTC(1/4)
755 GAAGAG
HaeII 566 GGCGCC [GA]GCGC^[CT]
782 GGCGCT
HaeIII 101 GGCC GG^CC
233 GGCC
HhaI 567 GCGC GCG^C
783 GCGC
HinP1I 567 GCGC G^CGC
783 GCGC
Ksp632I 405 CTCTTC CTCTTC(1/4)
755 GAAGAG
MaeI 341 CTAG C^TAG
622 CTAG
MaeIII 61 GTGAC ^GT[ACGT]AC
653 GTTAC
MwoI 7 GCGGCGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
13 GCGGCGGAAGC
NlaIV 566 GGCGCC GG[ACGT]^[ACGT]CC
734 GGAGCC
RsaI 348 GTAC GT^AC
575 GTAC
ScrFI 93 CCAGG CC^[ACGT]GG
642 CCGGG
TaqI 80 TCGA T^CGA
792 TCGA
TthHB8I 80 TCGA T^CGA
792 TCGA
XmnI 415 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
602 GAATGAATTC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AcsI AluI ApoI BsmAI
BsrI DpnII Fnu4HI HinfI HphI
ItaI MboI MboII MnlI NdeII
NlaIII Sau3AI TfiI Tsp509I TspRI