################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmFI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 211 GTCTAC GT^[AC][GT]AC AflIII 233 ACACGT A^C[GA][CT]GT AhdI 367 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 360 GATCC GGATC(4/5) AspEI 367 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 581 TGGCCA TGG^CCA BanII 603 GAGCCC G[GA]GC[CT]^C BbeI 266 GGCGCC GGCGC^C BciVI 53 GTATCC GTATCC(6/5) BclI 17 TGATCA T^GATCA BglII 524 AGATCT A^GATCT BsaI 624 GGTCTC GGTCTC(1/5) BseRI 334 GAGGAG GAGGAG(10/8) BsiEI 138 CGGCCG CG[GA][CT]^CG BsmBI 287 GAGACG CGTCTC(1/5) Bsp1407I 779 TGTACA T^GTACA BspMI 611 ACCTGC ACCTGC(4/8) BsrGI 779 TGTACA T^GTACA BsrI 6 ACTGG ACTGG(1/-1) BstEII 439 GGTGACC G^GT[ACGT]ACC BstPI 439 GGTGACC G^GT[ACGT]ACC EagI 138 CGGCCG C^GGCCG Eam1105I 367 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 571 CTCTTC CTCTTC(1/4) EclXI 138 CGGCCG C^GGCCG Eco52I 138 CGGCCG C^GGCCG EcoO65I 439 GGTGACC G^GT[ACGT]ACC FbaI 17 TGATCA T^GATCA KasI 266 GGCGCC G^GCGCC Ksp632I 571 CTCTTC CTCTTC(1/4) KspI 201 CCGCGG CCGC^GG MaeIII 440 GTGAC ^GT[ACGT]AC MluNI 581 TGGCCA TGG^CCA MscI 581 TGGCCA TGG^CCA MslI 297 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 266 GGCGCC GG^CGCC NcoI 578 CCATGG C^CATGG PvuII 484 CAGCTG CAG^CTG SacII 201 CCGCGG CCGC^GG SapI 570 GCTCTTC GCTCTTC(1/4) ScaI 635 AGTACT AGT^ACT SfaNI 731 GCATC GCATC(5/9) SfoI 266 GGCGCC GGC^GCC SmaI 714 CCCGGG CCC^GGG SmlI 382 CTCAAG C^T[CT][GA]AG SspBI 779 TGTACA T^GTACA Tsp45I 440 GTGAC ^GT[GC]AC XcmI 395 CCAAGGAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 714 CCCGGG C^CCGGG XmaI 714 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Aor51HI 506 AGCGCT AGC^GCT 827 AGCGCT AvaI 33 CCCGAG C^[CT]CG[GA]G 714 CCCGGG AvaII 465 GGACC G^G[AT]CC 552 GGACC BanI 266 GGCGCC G^G[CT][GA]CC 738 GGCACC BsmAI 287 GAGAC GTCTC(1/5) 625 GTCTC BsoBI 33 CCCGAG C^[CT]CG[GA]G 714 CCCGGG BssHII 566 GCGCGC G^CGCGC 748 GCGCGC BstYI 359 AGATCC [GA]^GATC[CT] 524 AGATCT DdeI 49 CTGAG C^T[ACGT]AG 349 CTCAG DsaI 201 CCGCGG C^C[GA][CT]GG 578 CCATGG Eco47III 506 AGCGCT AGC^GCT 827 AGCGCT FauI 256 CCCGC CCCGC(4/6) 684 GCGGG HinfI 40 GACTC G^A[ACGT]TC 325 GACTC HphI 439 GGTGA GGTGA(8/7) 134 TCACC MflI 359 AGATCC [GA]^GATC[CT] 524 AGATCT NlaIV 266 GGCGCC GG[ACGT]^[ACGT]CC 738 GGCACC PleI 40 GACTC GAGTC(4/5) 325 GACTC XhoII 359 AGATCC [GA]^GATC[CT] 524 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AfaI AluI BbvI BcnI BmyI BsaHI BsaJI BsgI BsiYI BslI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII RsaI Sau3AI Sau96I ScrFI StyI TaqI TseI TthHB8I