Restriction map for
pF1KB6889
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 432 GACGTC GACGT^C
AcyI 432 GACGTC G[GA]^CG[CT]C
AvaI 30 CTCGGG C^[CT]CG[GA]G
BanI 178 GGCACC G^G[CT][GA]CC
BbsI 109 GAAGAC GAAGAC(2/6)
BbvCI 403 GCTGAGG CCTCAGC(-5/-2)
BcgI 462 CGAGCTATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 168 GGATAC GTATCC(6/5)
BpuAI 109 GAAGAC GAAGAC(2/6)
BsaHI 432 GACGTC G[GA]^CG[CT]C
BsaI 375 GGTCTC GGTCTC(1/5)
BseRI 406 GAGGAG GAGGAG(10/8)
BsgI 61 GTGCAG GTGCAG(16/14)
BsiWI 380 CGTACG C^GTACG
BsmBI 430 GAGACG CGTCTC(1/5)
BsoBI 30 CTCGGG C^[CT]CG[GA]G
Bsp1407I 209 TGTACA T^GTACA
BsrGI 209 TGTACA T^GTACA
BstXI 329 CCATGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 161 AGATCC [GA]^GATC[CT]
DdeI 404 CTGAG C^T[ACGT]AG
EcoNI 399 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 193 GCGTC GACGC(5/10)
Hin1I 432 GACGTC G[GA]^CG[CT]C
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
HinfI 139 GACTC G^A[ACGT]TC
MaeII 433 ACGT A^CGT
MaeIII 70 GTGAC ^GT[ACGT]AC
MboII 109 GAAGA GAAGA(8/7)
MflI 161 AGATCC [GA]^GATC[CT]
MslI 300 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 139 GACTC GAGTC(4/5)
PpuMI 203 GGGACCT [GA]G^G[AT]CC[CT]
SfcI 243 CTACAG C^T[GA][CT]AG
SspBI 209 TGTACA T^GTACA
Tsp45I 70 GTGAC ^GT[GC]AC
XcmI 329 CCATGATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 161 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 241 GTCTAC GT^[AC][GT]AC
457 GTAGAC
BanII 27 GGGCTC G[GA]GC[CT]^C
75 GGGCTC
BpmI 157 CTGGAG CTGGAG(16/14)
358 CTGGAG
BsmAI 430 GAGAC GTCTC(1/5)
376 GTCTC
BsmFI 203 GGGAC GGGAC(10/14)
290 GGGAC
BstF5I 234 CATCC GGATG(2/0)
300 CATCC
DraII 203 GGGACCT [GA]G^G[ACGT]CC[CT]
395 AGGCCCT
EaeI 222 CGGCCA [CT]^GGCC[GA]
437 CGGCCA
EcoO109I 203 GGGACCT [GA]G^G[ACGT]CC[CT]
395 AGGCCCT
FokI 234 CATCC GGATG(9/13)
300 CATCC
HphI 364 GGTGA GGTGA(8/7)
56 TCACC
TaqI 86 TCGA T^CGA
107 TCGA
TthHB8I 86 TCGA T^CGA
107 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DpnII EcoRII
FauI Fnu4HI HaeIII HhaI HinP1I
ItaI MboI MnlI MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI