Restriction map for pF1KB8652
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BcnI        BfrI        BglI        BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaWI       BseAI       BsgI        BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         220  GACGTC               GACGT^C
AcsI          448  AAATTC               [GA]^AATT[CT]
AcyI          220  GACGTC               G[GA]^CG[CT]C
AlwI          508  GGATC                GGATC(4/5)
ApoI          448  AAATTC               [GA]^AATT[CT]
AvaII          27  GGACC                G^G[AT]CC
BalI          413  TGGCCA               TGG^CCA
BbvCI         589  GCTGAGG              CCTCAGC(-5/-2)
BbvI          408  GCTGC                GCAGC(8/12)
BclI          522  TGATCA               T^GATCA
BfaI          550  CTAG                 C^TAG
BglII         290  AGATCT               A^GATCT
BmyI          393  GTGCCC               G[AGT]GC[ACT]^C
BsaHI         220  GACGTC               G[GA]^CG[CT]C
BsaI          574  GAGACC               GGTCTC(1/5)
BsmAI         574  GAGAC                GTCTC(1/5)
BsmFI         275  GGGAC                GGGAC(10/14)
Bsp1286I      393  GTGCCC               G[AGT]GC[ACT]^C
BspHI          21  TCATGA               T^CATGA
BspMI         340  GCAGGT               ACCTGC(4/8)
BssKI         356  CCTGG                ^CC[ACGT]GG
BstNI         356  CCTGG                CC^[AT]GG
BstYI         290  AGATCT               [GA]^GATC[CT]
DraI          145  TTTAAA               TTT^AAA
EagI            5  CGGCCG               C^GGCCG
EclXI           5  CGGCCG               C^GGCCG
Eco52I          5  CGGCCG               C^GGCCG
EcoRII        356  CCTGG                ^CC[AT]GG
FauI          153  GCGGG                CCCGC(4/6)
FbaI          522  TGATCA               T^GATCA
Hin1I         220  GACGTC               G[GA]^CG[CT]C
HinfI          58  GAGTC                G^A[ACGT]TC
MaeI          550  CTAG                 C^TAG
MaeII         221  ACGT                 A^CGT
MaeIII        218  GTGAC                ^GT[ACGT]AC
MflI          290  AGATCT               [GA]^GATC[CT]
MluNI         413  TGGCCA               TGG^CCA
MscI          413  TGGCCA               TGG^CCA
MslI          312  CACGCCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          356  CCTGG                CC^[AT]GG
NlaIV         250  GGGGCC               GG[ACGT]^[ACGT]CC
NotI            4  GCGGCCGC             GC^GGCCGC
PleI           58  GAGTC                GAGTC(4/5)
RcaI           21  TCATGA               T^CATGA
ScrFI         356  CCTGG                CC^[ACGT]GG
SfaNI         586  GATGC                GCATC(5/9)
TaqI          485  TCGA                 T^CGA
TseI          408  GCTGC                G^C[AT]GC
Tsp45I        218  GTGAC                ^GT[GC]AC
TthHB8I       485  TCGA                 T^CGA
XhoII         290  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BsiEI           5  CGGCCG               CG[GA][CT]^CG
              348  CGACCG              
BsrI          120  ACTGG                ACTGG(1/-1)
              214  ACTGG               
Cac8I         153  GCGGGC               GC[ACGT]^[ACGT]GC
              411  GCTGGC              
Cfr13I         27  GGACC                G^G[ACGT]CC
              251  GGGCC               
EaeI            5  CGGCCG               [CT]^GGCC[GA]
              413  TGGCCA              
EcoT14I        11  CCAAGG               C^C[AT][AT]GG
              578  CCAAGG              
HphI          217  GGTGA                GGTGA(8/7)
              564  GGTGA               
MseI          113  TTAA                 T^TAA
              146  TTAA                
Sau96I         27  GGACC                G^G[ACGT]CC
              251  GGGCC               
StyI           11  CCAAGG               C^C[AT][AT]GG
              578  CCAAGG              
Tru9I         113  TTAA                 T^TAA
              146  TTAA                
Tsp509I       111  AATT                 ^AATT
              449  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BsaJI       BseRI       BsiYI       
BslI        BsmI        BstF5I      DdeI        DpnII       
EarI        Fnu4HI      FokI        HaeIII      ItaI        
Ksp632I     MboI        MboII       MnlI        MwoI        
NdeII       NlaIII      Sau3AI      TspRI       
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