################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 220 GACGTC GACGT^C AcsI 448 AAATTC [GA]^AATT[CT] AcyI 220 GACGTC G[GA]^CG[CT]C AlwI 508 GGATC GGATC(4/5) ApoI 448 AAATTC [GA]^AATT[CT] AvaII 27 GGACC G^G[AT]CC BalI 413 TGGCCA TGG^CCA BbvCI 589 GCTGAGG CCTCAGC(-5/-2) BbvI 408 GCTGC GCAGC(8/12) BclI 522 TGATCA T^GATCA BfaI 550 CTAG C^TAG BglII 290 AGATCT A^GATCT BmyI 393 GTGCCC G[AGT]GC[ACT]^C BsaHI 220 GACGTC G[GA]^CG[CT]C BsaI 574 GAGACC GGTCTC(1/5) BsmAI 574 GAGAC GTCTC(1/5) BsmFI 275 GGGAC GGGAC(10/14) Bsp1286I 393 GTGCCC G[AGT]GC[ACT]^C BspHI 21 TCATGA T^CATGA BspMI 340 GCAGGT ACCTGC(4/8) BssKI 356 CCTGG ^CC[ACGT]GG BstNI 356 CCTGG CC^[AT]GG BstYI 290 AGATCT [GA]^GATC[CT] DraI 145 TTTAAA TTT^AAA EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG EcoRII 356 CCTGG ^CC[AT]GG FauI 153 GCGGG CCCGC(4/6) FbaI 522 TGATCA T^GATCA Hin1I 220 GACGTC G[GA]^CG[CT]C HinfI 58 GAGTC G^A[ACGT]TC MaeI 550 CTAG C^TAG MaeII 221 ACGT A^CGT MaeIII 218 GTGAC ^GT[ACGT]AC MflI 290 AGATCT [GA]^GATC[CT] MluNI 413 TGGCCA TGG^CCA MscI 413 TGGCCA TGG^CCA MslI 312 CACGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 356 CCTGG CC^[AT]GG NlaIV 250 GGGGCC GG[ACGT]^[ACGT]CC NotI 4 GCGGCCGC GC^GGCCGC PleI 58 GAGTC GAGTC(4/5) RcaI 21 TCATGA T^CATGA ScrFI 356 CCTGG CC^[ACGT]GG SfaNI 586 GATGC GCATC(5/9) TaqI 485 TCGA T^CGA TseI 408 GCTGC G^C[AT]GC Tsp45I 218 GTGAC ^GT[GC]AC TthHB8I 485 TCGA T^CGA XhoII 290 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BsiEI 5 CGGCCG CG[GA][CT]^CG 348 CGACCG BsrI 120 ACTGG ACTGG(1/-1) 214 ACTGG Cac8I 153 GCGGGC GC[ACGT]^[ACGT]GC 411 GCTGGC Cfr13I 27 GGACC G^G[ACGT]CC 251 GGGCC EaeI 5 CGGCCG [CT]^GGCC[GA] 413 TGGCCA EcoT14I 11 CCAAGG C^C[AT][AT]GG 578 CCAAGG HphI 217 GGTGA GGTGA(8/7) 564 GGTGA MseI 113 TTAA T^TAA 146 TTAA Sau96I 27 GGACC G^G[ACGT]CC 251 GGGCC StyI 11 CCAAGG C^C[AT][AT]GG 578 CCAAGG Tru9I 113 TTAA T^TAA 146 TTAA Tsp509I 111 AATT ^AATT 449 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BsaJI BseRI BsiYI BslI BsmI BstF5I DdeI DpnII EarI Fnu4HI FokI HaeIII ItaI Ksp632I MboI MboII MnlI MwoI NdeII NlaIII Sau3AI TspRI