Restriction map for
pF1KE0474
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI Sau96I SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AcsI 262 AAATTT [GA]^AATT[CT]
AfaI 366 GTAC GT^AC
AflII 100 CTTAAG C^TTAAG
AlwNI 11 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 262 AAATTT [GA]^AATT[CT]
AspHI 209 GAGCTC G[AT]GC[AT]^C
BalI 193 TGGCCA TGG^CCA
BanI 321 GGCACC G^G[CT][GA]CC
BanII 209 GAGCTC G[GA]GC[CT]^C
BbvI 426 GCTGC GCAGC(8/12)
BfaI 187 CTAG C^TAG
BfrI 100 CTTAAG C^TTAAG
BglII 23 AGATCT A^GATCT
BpmI 158 CTCCAG CTGGAG(16/14)
BsiHKAI 209 GAGCTC G[AT]GC[AT]^C
BsrDI 487 CATTGC GCAATG(2/0)
BsrI 160 CCAGT ACTGG(1/-1)
BssKI 277 CCAGG ^CC[ACGT]GG
BstNI 277 CCAGG CC^[AT]GG
BstYI 23 AGATCT [GA]^GATC[CT]
DdeI 452 CTAAG C^T[ACGT]AG
DpnII 24 GATC ^GATC
EaeI 193 TGGCCA [CT]^GGCC[GA]
EarI 271 GAAGAG CTCTTC(1/4)
EcoRII 277 CCAGG ^CC[AT]GG
Fnu4HI 426 GCTGC GC^[ACGT]GC
HaeIII 194 GGCC GG^CC
HinfI 176 GAATC G^A[ACGT]TC
ItaI 426 GCTGC GC^[ACGT]GC
Ksp632I 271 GAAGAG CTCTTC(1/4)
MaeI 187 CTAG C^TAG
MaeII 182 ACGT A^CGT
MaeIII 115 GTGAC ^GT[ACGT]AC
MboI 24 GATC ^GATC
MflI 23 AGATCT [GA]^GATC[CT]
MluNI 193 TGGCCA TGG^CCA
MnlI 94 GAGG CCTC(7/6)
MscI 193 TGGCCA TGG^CCA
MslI 487 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 424 CAGCTG C[AC]G^C[GT]G
MvaI 277 CCAGG CC^[AT]GG
NdeI 146 CATATG CA^TATG
NdeII 24 GATC ^GATC
NlaIII 39 CATG CATG^
NlaIV 321 GGCACC GG[ACGT]^[ACGT]CC
NspI 38 ACATGC [GA]CATG^[CT]
PvuII 424 CAGCTG CAG^CTG
RsaI 366 GTAC GT^AC
SacI 209 GAGCTC GAGCT^C
SapI 271 GAAGAGC GCTCTTC(1/4)
Sau3AI 24 GATC ^GATC
ScrFI 277 CCAGG CC^[ACGT]GG
SmlI 100 CTTAAG C^T[CT][GA]AG
TaqI 129 TCGA T^CGA
TfiI 176 GAATC G^A[AT]TC
TseI 426 GCTGC G^C[AT]GC
Tsp45I 115 GTGAC ^GT[GC]AC
TspRI 293 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 129 TCGA T^CGA
XbaI 186 TCTAGA T^CTAGA
XhoII 23 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 4 enzymes) #
###################################
BmyI 209 GAGCTC G[AGT]GC[ACT]^C
320 GGGCAC
Bsp1286I 209 GAGCTC G[AGT]GC[ACT]^C
320 GGGCAC
MboII 271 GAAGA GAAGA(8/7)
370 GAAGA
Tsp509I 263 AATT ^AATT
415 AATT
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
AluI MseI SfaNI Tru9I