##################################
# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MfeI MluI MroI MspA1I MspI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuII RcaI
RsrII SacI SacII SapI Sau96I
SbfI ScaI ScrFI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 301 GTCGAC GT^[AC][GT]AC
AfaI 24 GTAC GT^AC
AflIII 347 ACATGT A^C[GA][CT]GT
AluI 284 AGCT AG^CT
AlwI 178 GATCC GGATC(4/5)
AspI 37 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvI 3 GCAGC GCAGC(8/12)
BglII 471 AGATCT A^GATCT
BpmI 475 CTGGAG CTGGAG(16/14)
BsaJI 259 CCAAGG C^C[ACGT][ACGT]GG
BsiEI 27 CGATCG CG[GA][CT]^CG
BsmAI 119 GTCTC GTCTC(1/5)
BsmFI 35 GGGAC GGGAC(10/14)
BspLU11I 347 ACATGT A^CATGT
BstYI 471 AGATCT [GA]^GATC[CT]
Cac8I 6 GCCTGC GC[ACGT]^[ACGT]GC
DraI 147 TTTAAA TTT^AAA
EcoT14I 259 CCAAGG C^C[AT][AT]GG
Fnu4HI 3 GCAGC GC^[ACGT]GC
HindIII 283 AAGCTT A^AGCTT
HpaI 264 GTTAAC GTT^AAC
HphI 394 GGTGA GGTGA(8/7)
ItaI 3 GCAGC GC^[ACGT]GC
MflI 471 AGATCT [GA]^GATC[CT]
MslI 145 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 285 GCTTAATTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 347 ACATGT [GA]CATG^[CT]
PflFI 37 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 27 CGATCG CGAT^CG
RsaI 24 GTAC GT^AC
SalI 301 GTCGAC G^TCGAC
StyI 259 CCAAGG C^C[AT][AT]GG
SwaI 146 ATTTAAAT ATTT^AAAT
TseI 3 GCAGC G^C[AT]GC
Tth111I 37 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 471 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 52 GAATTT [GA]^AATT[CT]
87 AAATTT
ApoI 52 GAATTT [GA]^AATT[CT]
87 AAATTT
BalI 246 TGGCCA TGG^CCA
256 TGGCCA
BsrI 323 ACTGG ACTGG(1/-1)
249 CCAGT
BstF5I 75 GGATG GGATG(2/0)
402 GGATG
DdeI 295 CTTAG C^T[ACGT]AG
371 CTGAG
EaeI 246 TGGCCA [CT]^GGCC[GA]
256 TGGCCA
FokI 75 GGATG GGATG(9/13)
402 GGATG
HaeIII 247 GGCC GG^CC
257 GGCC
HincII 264 GTTAAC GT[CT]^[GA]AC
301 GTCGAC
HindII 264 GTTAAC GT[CT]^[GA]AC
301 GTCGAC
MluNI 246 TGGCCA TGG^CCA
256 TGGCCA
MnlI 125 GAGG CCTC(7/6)
400 GAGG
MscI 246 TGGCCA TGG^CCA
256 TGGCCA
TaqI 30 TCGA T^CGA
302 TCGA
TspRI 169 CACTG CA[GC]TG[ACGT][ACGT]^
450 CAGTG
TthHB8I 30 TCGA T^CGA
302 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BbsI BpuAI DpnII HinfI MboI
MboII MseI NdeII NlaIII Sau3AI
TfiI Tru9I Tsp509I