Restriction map for
pF1KB6532
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I EarI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT14I FauI
FseI FspI Hin1I KasI KpnI
Ksp632I KspI MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
PstI PvuI RsrII SacI SacII
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AclI 765 AACGTT AA^CGTT
AcsI 202 AAATTC [GA]^AATT[CT]
AflII 76 CTTAAG C^TTAAG
AflIII 362 ACATGT A^C[GA][CT]GT
ApoI 202 AAATTC [GA]^AATT[CT]
AspHI 632 GTGCTC G[AT]GC[AT]^C
BbsI 1093 GAAGAC GAAGAC(2/6)
BbvI 867 GCTGC GCAGC(8/12)
BcnI 426 CCGGG CC^[GC]GG
BfrI 76 CTTAAG C^TTAAG
BglII 1023 AGATCT A^GATCT
BmyI 632 GTGCTC G[AGT]GC[ACT]^C
BpmI 1138 CTGGAG CTGGAG(16/14)
BpuAI 1093 GAAGAC GAAGAC(2/6)
BsiEI 69 CGGCCG CG[GA][CT]^CG
BsiHKAI 632 GTGCTC G[AT]GC[AT]^C
BsmAI 366 GTCTC GTCTC(1/5)
BsmFI 953 GTCCC GGGAC(10/14)
Bsp1286I 632 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 362 ACATGT A^CATGT
BssSI 551 CACGAG CACGAG(-5/-1)
BstAPI 1060 GCAATTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 22 GCGC GCG^C
EaeI 69 CGGCCG [CT]^GGCC[GA]
EagI 69 CGGCCG C^GGCCG
EclXI 69 CGGCCG C^GGCCG
Eco52I 69 CGGCCG C^GGCCG
EcoNI 496 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 681 ATGCAT ATGCA^T
Fnu4HI 867 GCTGC GC^[ACGT]GC
HaeII 21 GGCGCT [GA]GCGC^[CT]
HapII 426 CCGG C^CGG
HgaI 870 GCGTC GACGC(5/10)
HhaI 22 GCGC GCG^C
HinP1I 22 GCGC G^CGC
HindIII 997 AAGCTT A^AGCTT
HpaI 189 GTTAAC GTT^AAC
HpaII 426 CCGG C^CGG
ItaI 867 GCTGC GC^[ACGT]GC
MaeII 766 ACGT A^CGT
MaeIII 777 GTTAC ^GT[ACGT]AC
MfeI 989 CAATTG C^AATTG
MslI 687 CATTTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 426 CCGG C^CGG
MunI 989 CAATTG C^AATTG
MwoI 1060 GCAATTCGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 426 CCGGG CC^[GC]GG
NheI 562 GCTAGC G^CTAGC
NsiI 681 ATGCAT ATGCA^T
NspI 362 ACATGT [GA]CATG^[CT]
PleI 1141 GAGTC GAGTC(4/5)
PshAI 67 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 765 AACGTT AA^CGTT
SalI 251 GTCGAC G^TCGAC
SexAI 525 ACCAGGT A^CC[AT]GGT
SfcI 148 CTACAG C^T[GA][CT]AG
SmlI 76 CTTAAG C^T[CT][GA]AG
TseI 867 GCTGC G^C[AT]GC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 251 GTCGAC GT^[AC][GT]AC
610 GTAGAC
AciI 36 CCGC CCGC(-3/-1)
4 GCGG
BamHI 345 GGATCC G^GATCC
855 GGATCC
BsaJI 378 CCCTGG C^C[ACGT][ACGT]GG
379 CCTGGG
BspMI 949 GCAGGT ACCTGC(4/8)
1048 GCAGGT
BsrI 351 ACTGG ACTGG(1/-1)
852 ACTGG
Cac8I 562 GCTAGC GC[ACGT]^[ACGT]GC
566 GCCAGC
HaeIII 70 GGCC GG^CC
144 GGCC
HincII 189 GTTAAC GT[CT]^[GA]AC
251 GTCGAC
HindII 189 GTTAAC GT[CT]^[GA]AC
251 GTCGAC
MspA1I 568 CAGCTG C[AC]G^C[GT]G
743 CAGCTG
PpuMI 374 AGGACCC [GA]G^G[AT]CC[CT]
951 AGGTCCC
PvuII 568 CAGCTG CAG^CTG
743 CAGCTG
SfaNI 706 GATGC GCATC(5/9)
683 GCATC
TaqI 65 TCGA T^CGA
252 TCGA
TspRI 350 CACTG CA[GC]TG[ACGT][ACGT]^
851 CACTG
TthHB8I 65 TCGA T^CGA
252 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AlwI AvaII BclI
BfaI BsiYI BslI BspHI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FbaI
FokI HinfI HphI MaeI MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
Tsp509I XhoII