Restriction map for
pF1KB6588
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaAI BsaHI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MluI MluNI MscI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 770 CGCG CG^CG
AccIII 125 TCCGGA T^CCGGA
AflIII 1193 ACATGT A^C[GA][CT]GT
Alw44I 1086 GTGCAC G^TGCAC
ApaLI 1086 GTGCAC G^TGCAC
BbsI 585 GAAGAC GAAGAC(2/6)
BcgI 150 CGAACTAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 585 GAAGAC GAAGAC(2/6)
BsaBI 891 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 125 TCCGGA [AT]^CCGG[AT]
BseAI 125 TCCGGA T^CCGGA
BsmAI 640 GTCTC GTCTC(1/5)
BsmFI 794 GGGAC GGGAC(10/14)
BspEI 125 TCCGGA T^CCGGA
BspLU11I 1193 ACATGT A^CATGT
BsrDI 537 CATTGC GCAATG(2/0)
BstBI 523 TTCGAA TT^CGAA
BstUI 770 CGCG CG^CG
BstYI 374 AGATCC [GA]^GATC[CT]
DsaI 236 CCATGG C^C[GA][CT]GG
EarI 520 CTCTTC CTCTTC(1/4)
EcoRI 946 GAATTC G^AATTC
EcoRV 895 GATATC GAT^ATC
FauI 177 GCGGG CCCGC(4/6)
HaeII 3 GGCGCT [GA]GCGC^[CT]
HphI 690 GGTGA GGTGA(8/7)
Ksp632I 520 CTCTTC CTCTTC(1/4)
MaeII 293 ACGT A^CGT
MamI 891 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 330 CAATTG C^AATTG
MflI 374 AGATCC [GA]^GATC[CT]
MroI 125 TCCGGA T^CCGGA
MseI 506 TTAA T^TAA
MslI 735 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 330 CAATTG C^AATTG
MvnI 770 CGCG CG^CG
NcoI 236 CCATGG C^CATGG
NspV 523 TTCGAA TT^CGAA
PflMI 574 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 519 GCTCTTC GCTCTTC(1/4)
SfuI 523 TTCGAA TT^CGAA
SspI 65 AATATT AAT^ATT
Tru9I 506 TTAA T^TAA
Van91I 574 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 329 CCAATTGCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 374 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 289 GTAGAC GT^[AC][GT]AC
391 GTAGAC
AlwI 375 GATCC GGATC(4/5)
1022 GGATC
AvaI 599 CCCGGG C^[CT]CG[GA]G
1016 CCCGGG
AvaII 13 GGACC G^G[AT]CC
568 GGACC
BlpI 541 GCTCAGC GC^T[ACGT]AGC
1042 GCTGAGC
Bpu1102I 541 GCTCAGC GC^T[ACGT]AGC
1042 GCTGAGC
BsgI 367 CTGCAC GTGCAG(16/14)
805 GTGCAG
BsoBI 599 CCCGGG C^[CT]CG[GA]G
1016 CCCGGG
BstF5I 268 CATCC GGATG(2/0)
511 CATCC
CelII 541 GCTCAGC GC^T[ACGT]AGC
1042 GCTGAGC
FokI 268 CATCC GGATG(9/13)
511 CATCC
NlaIV 208 GGCTCC GG[ACGT]^[ACGT]CC
763 GGCTCC
PstI 133 CTGCAG CTGCA^G
196 CTGCAG
PvuII 94 CAGCTG CAG^CTG
190 CAGCTG
SacI 517 GAGCTC GAGCT^C
1045 GAGCTC
SmaI 599 CCCGGG CCC^GGG
1016 CCCGGG
TfiI 433 GATTC G^A[AT]TC
990 GATTC
XmaCI 599 CCCGGG C^CCGGG
1016 CCCGGG
XmaI 599 CCCGGG C^CCGGG
1016 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I