Restriction map for
pF1KE0481
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 1007 GAATTT [GA]^AATT[CT]
AfaI 201 GTAC GT^AC
AlwNI 1196 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1007 GAATTT [GA]^AATT[CT]
AseI 1102 ATTAAT AT^TAAT
AsnI 1102 ATTAAT AT^TAAT
Asp700I 1108 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 338 CCCGGG C^[CT]CG[GA]G
BfaI 392 CTAG C^TAG
BsaI 478 GAGACC GGTCTC(1/5)
BsgI 24 CTGCAC GTGCAG(16/14)
BsiYI 809 CCTACATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 809 CCTACATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 338 CCCGGG C^[CT]CG[GA]G
BspMI 86 ACCTGC ACCTGC(4/8)
BssSI 900 CACGAG CACGAG(-5/-1)
Bst1107I 627 GTATAC GTA^TAC
BstZ17I 627 GTATAC GTA^TAC
CfoI 33 GCGC GCG^C
DraI 72 TTTAAA TTT^AAA
DsaI 482 CCATGG C^C[GA][CT]GG
EcoT14I 482 CCATGG C^C[AT][AT]GG
EcoT22I 311 ATGCAT ATGCA^T
FauI 1166 GCGGG CCCGC(4/6)
HaeIII 275 GGCC GG^CC
HhaI 33 GCGC GCG^C
HinP1I 33 GCGC G^CGC
MaeI 392 CTAG C^TAG
MaeII 408 ACGT A^CGT
MboII 153 TCTTC GAAGA(8/7)
NcoI 482 CCATGG C^CATGG
NdeI 242 CATATG CA^TATG
NsiI 311 ATGCAT ATGCA^T
PshBI 1102 ATTAAT AT^TAAT
PstI 632 CTGCAG CTGCA^G
RsaI 201 GTAC GT^AC
SfcI 632 CTGCAG C^T[GA][CT]AG
SmaI 338 CCCGGG CCC^GGG
StyI 482 CCATGG C^C[AT][AT]GG
TaqI 1232 TCGA T^CGA
TthHB8I 1232 TCGA T^CGA
XcmI 473 CCAGGGAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 338 CCCGGG C^CCGGG
XmaI 338 CCCGGG C^CCGGG
XmnI 1108 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 250 GTAGAC GT^[AC][GT]AC
627 GTATAC
AspI 685 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
964 GACCCAGTC
BamHI 352 GGATCC G^GATCC
878 GGATCC
BanII 49 GGGCTC G[GA]GC[CT]^C
1168 GGGCTC
BbvI 458 GCAGC GCAGC(8/12)
714 GCTGC
BmyI 49 GGGCTC G[AGT]GC[ACT]^C
1168 GGGCTC
BseRI 703 CTCCTC GAGGAG(10/8)
395 GAGGAG
Bsp1286I 49 GGGCTC G[AGT]GC[ACT]^C
1168 GGGCTC
BsrFI 187 ACCGGC [GA]^CCGG[CT]
1080 ACCGGC
BstYI 352 GGATCC [GA]^GATC[CT]
878 GGATCC
Cfr10I 187 ACCGGC [GA]^CCGG[CT]
1080 ACCGGC
HgaI 346 GACGC GACGC(5/10)
523 GACGC
MflI 352 GGATCC [GA]^GATC[CT]
878 GGATCC
NlaIII 483 CATG CATG^
1224 CATG
PflFI 685 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
964 GACCCAGTC
TfiI 180 GAATC G^A[AT]TC
699 GAATC
TseI 458 GCAGC G^C[AT]GC
714 GCTGC
Tth111I 685 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
964 GACCCAGTC
XhoII 352 GGATCC [GA]^GATC[CT]
878 GGATCC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AvaII BcnI
BpmI BsaJI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI MaeIII MboI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIV PleI PpuMI Sau3AI
Sau96I ScrFI SfaNI Tru9I Tsp45I
Tsp509I TspRI