Restriction map for
pF1KB6515
##################################
# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StyI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AciI 885 GCGG CCGC(-3/-1)
AflII 996 CTTAAG C^TTAAG
AflIII 442 ACATGT A^C[GA][CT]GT
AlwI 879 GGATC GGATC(4/5)
AlwNI 162 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 836 CTCGAG C^[CT]CG[GA]G
BanI 257 GGTGCC G^G[CT][GA]CC
BanII 918 GAGCCC G[GA]GC[CT]^C
BclI 488 TGATCA T^GATCA
BfrI 996 CTTAAG C^TTAAG
BglII 690 AGATCT A^GATCT
BmyI 918 GAGCCC G[AGT]GC[ACT]^C
BsiYI 427 CCAAGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 427 CCAAGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 783 CGTCTC CGTCTC(1/5)
BsoBI 836 CTCGAG C^[CT]CG[GA]G
Bsp1286I 918 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 442 ACATGT A^CATGT
BstF5I 179 CATCC GGATG(2/0)
FbaI 488 TGATCA T^GATCA
FokI 179 CATCC GGATG(9/13)
HaeIII 993 GGCC GG^CC
HgaI 987 GACGC GACGC(5/10)
HindIII 742 AAGCTT A^AGCTT
MaeII 848 ACGT A^CGT
MslI 448 CACATGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 836 CTCGAG C^TCGAG
PflMI 427 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 211 GATGC GCATC(5/9)
SpeI 774 ACTAGT A^CTAGT
StuI 992 AGGCCT AGG^CCT
TfiI 768 GAATC G^A[AT]TC
Tsp45I 446 GTCAC ^GT[GC]AC
Van91I 427 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 836 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 649 GGACC G^G[AT]CC
667 GGACC
BbvI 795 GCAGC GCAGC(8/12)
1061 GCAGC
BsaJI 495 CCCTGG C^C[ACGT][ACGT]GG
921 CCCAGG
BsrI 623 CCAGT ACTGG(1/-1)
979 CCAGT
BstYI 690 AGATCT [GA]^GATC[CT]
879 GGATCT
Cfr13I 649 GGACC G^G[ACGT]CC
667 GGACC
Fnu4HI 795 GCAGC GC^[ACGT]GC
1061 GCAGC
ItaI 795 GCAGC GC^[ACGT]GC
1061 GCAGC
MflI 690 AGATCT [GA]^GATC[CT]
879 GGATCT
MspA1I 252 CAGCTG C[AC]G^C[GT]G
1012 CAGCTG
NspI 442 ACATGT [GA]CATG^[CT]
798 GCATGT
PvuII 252 CAGCTG CAG^CTG
1012 CAGCTG
Sau96I 649 GGACC G^G[ACGT]CC
667 GGACC
SmlI 836 CTCGAG C^T[CT][GA]AG
996 CTTAAG
TseI 795 GCAGC G^C[AT]GC
1061 GCAGC
XhoII 690 AGATCT [GA]^GATC[CT]
879 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI BbsI BfaI BpmI BpuAI
BseRI BsmAI BssKI BstNI Cac8I
DdeI DpnII EcoRII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI ScrFI TaqI
Tru9I Tsp509I TspRI TthHB8I