Restriction map for
pF1KB5772
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BcgI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI BstAPI BstBI BstUI
BstXI CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AlwNI 499 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2035 CCTGAGG CC^T[ACGT]AGG
BbsI 321 GAAGAC GAAGAC(2/6)
BciVI 850 GTATCC GTATCC(6/5)
BclI 1020 TGATCA T^GATCA
BcnI 392 CCCGG CC^[GC]GG
BlpI 379 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 379 GCTGAGC GC^T[ACGT]AGC
BpuAI 321 GAAGAC GAAGAC(2/6)
BsaI 336 GGTCTC GGTCTC(1/5)
BsaWI 317 ACCGGA [AT]^CCGG[AT]
Bsp1407I 718 TGTACA T^GTACA
BspMI 1697 ACCTGC ACCTGC(4/8)
BsrBI 136 GAGCGG CCGCTC(-3/-3)
BsrDI 192 CATTGC GCAATG(2/0)
BsrGI 718 TGTACA T^GTACA
Bst1107I 1576 GTATAC GTA^TAC
BstEII 1762 GGTTACC G^GT[ACGT]ACC
BstPI 1762 GGTTACC G^GT[ACGT]ACC
BstZ17I 1576 GTATAC GTA^TAC
Bsu36I 2035 CCTGAGG CC^T[ACGT]AGG
CelII 379 GCTGAGC GC^T[ACGT]AGC
DraII 2199 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 2035 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2199 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1762 GGTTACC G^GT[ACGT]ACC
EcoRI 1910 GAATTC G^AATTC
EcoT14I 648 CCAAGG C^C[AT][AT]GG
EcoT22I 546 ATGCAT ATGCA^T
FbaI 1020 TGATCA T^GATCA
MfeI 714 CAATTG C^AATTG
MunI 714 CAATTG C^AATTG
NciI 392 CCCGG CC^[GC]GG
NdeI 259 CATATG CA^TATG
NsiI 546 ATGCAT ATGCA^T
PpuMI 2199 GGGACCC [GA]G^G[AT]CC[CT]
SalI 632 GTCGAC G^TCGAC
SapI 1183 GAAGAGC GCTCTTC(1/4)
SphI 1132 GCATGC GCATG^C
SspBI 718 TGTACA T^GTACA
StyI 648 CCAAGG C^C[AT][AT]GG
XbaI 1749 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BalI 188 TGGCCA TGG^CCA
1322 TGGCCA
BamHI 516 GGATCC G^GATCC
1508 GGATCC
BbvCI 520 CCTCAGC CCTCAGC(-5/-2)
1756 GCTGAGG
BsaJI 648 CCAAGG C^C[ACGT][ACGT]GG
2014 CCCAGG
BsmAI 1724 GAGAC GTCTC(1/5)
337 GTCTC
BsmI 544 GAATGC GAATGC(1/-1)
2246 GAATGC
EaeI 188 TGGCCA [CT]^GGCC[GA]
1322 TGGCCA
HapII 318 CCGG C^CGG
393 CCGG
HpaII 318 CCGG C^CGG
393 CCGG
MluNI 188 TGGCCA TGG^CCA
1322 TGGCCA
MscI 188 TGGCCA TGG^CCA
1322 TGGCCA
MslI 37 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
672 CATCCCTATG
MspI 318 CCGG C^CGG
393 CCGG
PstI 244 CTGCAG CTGCA^G
1045 CTGCAG
StuI 284 AGGCCT AGG^CCT
1592 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI