Restriction map for
pF1KB6582
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HpaI KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 91 GAATTC [GA]^AATT[CT]
AcyI 217 GACGCC G[GA]^CG[CT]C
AhdI 229 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 169 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1114 GGGCCC GGGCC^C
ApoI 91 GAATTC [GA]^AATT[CT]
AspEI 229 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1046 CCTCAGC CCTCAGC(-5/-2)
BclI 429 TGATCA T^GATCA
BglII 839 AGATCT A^GATCT
BpmI 1182 CTCCAG CTGGAG(16/14)
BsaBI 337 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 217 GACGCC G[GA]^CG[CT]C
BsaI 307 GAGACC GGTCTC(1/5)
BseRI 241 GAGGAG GAGGAG(10/8)
BsmI 1096 GCATTC GAATGC(1/-1)
BspHI 1130 TCATGA T^CATGA
BsrBI 582 CCGCTC CCGCTC(-3/-3)
BstEII 1023 GGTCACC G^GT[ACGT]ACC
BstPI 1023 GGTCACC G^GT[ACGT]ACC
CpoI 203 CGGACCG CG^G[AT]CCG
DsaI 606 CCGTGG C^C[GA][CT]GG
Eam1105I 229 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 117 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1023 GGTCACC G^GT[ACGT]ACC
EcoRI 91 GAATTC G^AATTC
FbaI 429 TGATCA T^GATCA
HgaI 217 GACGC GACGC(5/10)
Hin1I 217 GACGCC G[GA]^CG[CT]C
HindIII 395 AAGCTT A^AGCTT
MamI 337 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 492 GCATGT [GA]CATG^[CT]
PflMI 168 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 40 CTGCAG CTGCA^G
PvuII 1162 CAGCTG CAG^CTG
RcaI 1130 TCATGA T^CATGA
RsrII 203 CGGACCG CG^G[AT]CCG
SfcI 40 CTGCAG C^T[GA][CT]AG
SmlI 446 CTCAAG C^T[CT][GA]AG
Tsp509I 92 AATT ^AATT
TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 168 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 121 CGCG CG^CG
130 CGCG
AspI 82 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
205 GACCGTGTC
AvaI 645 CCCGAG C^[CT]CG[GA]G
1204 CTCGGG
AvaII 204 GGACC G^G[AT]CC
696 GGACC
BanII 25 GGGCTC G[GA]GC[CT]^C
1114 GGGCCC
BsmAI 307 GAGAC GTCTC(1/5)
518 GAGAC
BsmFI 227 GGGAC GGGAC(10/14)
690 GGGAC
BsoBI 645 CCCGAG C^[CT]CG[GA]G
1204 CTCGGG
Bsp1407I 635 TGTACA T^GTACA
1089 TGTACA
BsrGI 635 TGTACA T^GTACA
1089 TGTACA
BsrI 693 ACTGG ACTGG(1/-1)
265 CCAGT
BstUI 121 CGCG CG^CG
130 CGCG
CfoI 120 GCGC GCG^C
131 GCGC
DraII 1113 GGGGCCC [GA]G^G[ACGT]CC[CT]
1114 GGGCCCT
EcoO109I 1113 GGGGCCC [GA]G^G[ACGT]CC[CT]
1114 GGGCCCT
EcoT14I 346 CCAAGG C^C[AT][AT]GG
504 CCAAGG
HhaI 120 GCGC GCG^C
131 GCGC
HinP1I 120 GCGC G^CGC
131 GCGC
MseI 281 TTAA T^TAA
785 TTAA
MspA1I 984 CCGCTG C[AC]G^C[GT]G
1162 CAGCTG
MvnI 121 CGCG CG^CG
130 CGCG
PflFI 82 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
205 GACCGTGTC
ScaI 104 AGTACT AGT^ACT
1001 AGTACT
SspBI 635 TGTACA T^GTACA
1089 TGTACA
StyI 346 CCAAGG C^C[AT][AT]GG
504 CCAAGG
Tru9I 281 TTAA T^TAA
785 TTAA
Tsp45I 703 GTGAC ^GT[GC]AC
1024 GTCAC
Tth111I 82 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
205 GACCGTGTC
XcmI 504 CCAAGGAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1184 CCAGCGTGGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI TseI TthHB8I
XhoII