Restriction map for
pF1KB5408
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AvaI
AviII BamHI BbrPI BbvCI BciVI
BclI BglII BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MflI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 61 GTCGAC GT^[AC][GT]AC
AccII 51 CGCG CG^CG
AflII 649 CTTAAG C^TTAAG
Alw44I 133 GTGCAC G^TGCAC
ApaLI 133 GTGCAC G^TGCAC
AseI 157 ATTAAT AT^TAAT
AsnI 157 ATTAAT AT^TAAT
AspI 882 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 942 CCTAGG C^CTAGG
BalI 894 TGGCCA TGG^CCA
BbsI 598 GAAGAC GAAGAC(2/6)
BcgI 336 GCAGGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 649 CTTAAG C^TTAAG
BglI 1149 GCCCTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 942 CCTAGG C^CTAGG
BpuAI 598 GAAGAC GAAGAC(2/6)
BsaAI 1009 TACGTG [CT]AC^GT[GA]
BseRI 98 GAGGAG GAGGAG(10/8)
BsmBI 906 CGTCTC CGTCTC(1/5)
BsrBI 386 GAGCGG CCGCTC(-3/-3)
BssSI 400 CACGAG CACGAG(-5/-1)
BstEII 630 GGTAACC G^GT[ACGT]ACC
BstPI 630 GGTAACC G^GT[ACGT]ACC
BstUI 51 CGCG CG^CG
EaeI 894 TGGCCA [CT]^GGCC[GA]
EarI 790 CTCTTC CTCTTC(1/4)
EcoNI 555 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 630 GGTAACC G^GT[ACGT]ACC
FauI 91 GCGGG CCCGC(4/6)
HincII 61 GTCGAC GT[CT]^[GA]AC
HindII 61 GTCGAC GT[CT]^[GA]AC
Ksp632I 790 CTCTTC CTCTTC(1/4)
MluNI 894 TGGCCA TGG^CCA
MscI 894 TGGCCA TGG^CCA
MvnI 51 CGCG CG^CG
NaeI 5 GCCGGC GCC^GGC
NcoI 1023 CCATGG C^CATGG
NgoMIV 5 GCCGGC G^CCGGC
PflFI 882 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 788 GACTC GAGTC(4/5)
PshBI 157 ATTAAT AT^TAAT
SacI 1078 GAGCTC GAGCT^C
SalI 61 GTCGAC G^TCGAC
SbfI 746 CCTGCAGG CCTGCA^GG
Sse8387I 746 CCTGCAGG CCTGCA^GG
TfiI 928 GAATC G^A[AT]TC
Tth111I 882 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 212 CCACCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcsI 426 GAATTT [GA]^AATT[CT]
803 AAATTC
AfaI 27 GTAC GT^AC
48 GTAC
AlwI 460 GATCC GGATC(4/5)
672 GGATC
AlwNI 271 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
956 CAGTCACTG
ApoI 426 GAATTT [GA]^AATT[CT]
803 AAATTC
AspHI 133 GTGCAC G[AT]GC[AT]^C
1078 GAGCTC
BanII 32 GGGCTC G[GA]GC[CT]^C
1078 GAGCTC
BsiHKAI 133 GTGCAC G[AT]GC[AT]^C
1078 GAGCTC
BsmAI 202 GAGAC GTCTC(1/5)
907 GTCTC
BsmFI 644 GGGAC GGGAC(10/14)
1089 GTCCC
BspMI 749 GCAGGT ACCTGC(4/8)
759 GCAGGT
BsrFI 5 GCCGGC [GA]^CCGG[CT]
322 GCCGGT
BstXI 215 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
771 CCAGCCTGCTGG
Cfr10I 5 GCCGGC [GA]^CCGG[CT]
322 GCCGGT
DdeI 415 CTCAG C^T[ACGT]AG
1058 CTGAG
DpnII 460 GATC ^GATC
673 GATC
DsaI 635 CCGTGG C^C[GA][CT]GG
1023 CCATGG
HinfI 788 GACTC G^A[ACGT]TC
928 GAATC
MaeII 530 ACGT A^CGT
1010 ACGT
MboI 460 GATC ^GATC
673 GATC
MseI 158 TTAA T^TAA
650 TTAA
MslI 187 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1029 CATTGTGGTG
NdeII 460 GATC ^GATC
673 GATC
PpuMI 644 GGGACCT [GA]G^G[AT]CC[CT]
1087 GGGTCCC
RsaI 27 GTAC GT^AC
48 GTAC
Sau3AI 460 GATC ^GATC
673 GATC
SfaNI 259 GATGC GCATC(5/9)
1140 GCATC
SmlI 168 CTTGAG C^T[CT][GA]AG
649 CTTAAG
Tru9I 158 TTAA T^TAA
650 TTAA
Tsp509I 427 AATT ^AATT
804 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BbeI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NarI
NciI NlaIII NlaIV PstI PvuII
Sau96I ScrFI SfcI SfoI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I