Restriction map for
pF1KB6503
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KspI
MamI MfeI MluI MroI MseI
MunI NarI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SbfI ScaI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 665 GGTACC G^GTACC
AflIII 857 ACATGT A^C[GA][CT]GT
Alw44I 308 GTGCAC G^TGCAC
AocI 502 CCTGAGG CC^T[ACGT]AGG
ApaLI 308 GTGCAC G^TGCAC
Asp718I 665 GGTACC G^GTACC
BalI 464 TGGCCA TGG^CCA
BanI 665 GGTACC G^G[CT][GA]CC
BanII 805 GAGCCC G[GA]GC[CT]^C
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BciVI 872 GTATCC GTATCC(6/5)
BfaI 334 CTAG C^TAG
BsaI 973 GGTCTC GGTCTC(1/5)
BsmI 277 GAATGC GAATGC(1/-1)
BspHI 231 TCATGA T^CATGA
BspLU11I 857 ACATGT A^CATGT
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrFI 715 GCCGGC [GA]^CCGG[CT]
BstYI 86 AGATCC [GA]^GATC[CT]
Bsu36I 502 CCTGAGG CC^T[ACGT]AGG
Cfr10I 715 GCCGGC [GA]^CCGG[CT]
DraIII 814 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 464 TGGCCA [CT]^GGCC[GA]
EarI 341 CTCTTC CTCTTC(1/4)
Eco81I 502 CCTGAGG CC^T[ACGT]AGG
EcoT22I 279 ATGCAT ATGCA^T
FauI 293 CCCGC CCCGC(4/6)
HaeII 940 AGCGCC [GA]GCGC^[CT]
KpnI 665 GGTACC GGTAC^C
Ksp632I 341 CTCTTC CTCTTC(1/4)
MaeI 334 CTAG C^TAG
MaeII 1066 ACGT A^CGT
MaeIII 1063 GTCAC ^GT[ACGT]AC
MflI 86 AGATCC [GA]^GATC[CT]
MluNI 464 TGGCCA TGG^CCA
MscI 464 TGGCCA TGG^CCA
MslI 978 CATCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 715 GCCGGC GCC^GGC
NgoMIV 715 GCCGGC G^CCGGC
NsiI 279 ATGCAT ATGCA^T
PpuMI 421 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 231 TCATGA T^CATGA
SapI 340 GCTCTTC GCTCTTC(1/4)
SexAI 424 ACCTGGT A^CC[AT]GGT
SmlI 670 CTTGAG C^T[CT][GA]AG
SpeI 333 ACTAGT A^CTAGT
TaqI 197 TCGA T^CGA
Tsp45I 1063 GTCAC ^GT[GC]AC
TthHB8I 197 TCGA T^CGA
XcmI 809 CCAGACACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 86 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 295 CGCG CG^CG
545 CGCG
AfaI 201 GTAC GT^AC
666 GTAC
AlwI 87 GATCC GGATC(4/5)
1020 GATCC
BbsI 894 GAAGAC GAAGAC(2/6)
646 GTCTTC
BcnI 299 CCGGG CC^[GC]GG
621 CCGGG
BpuAI 894 GAAGAC GAAGAC(2/6)
646 GTCTTC
BsaJI 723 CCAGGG C^C[ACGT][ACGT]GG
969 CCAGGG
BseRI 377 CTCCTC GAGGAG(10/8)
40 GAGGAG
BsmFI 475 GTCCC GGGAC(10/14)
946 GTCCC
BstUI 295 CGCG CG^CG
545 CGCG
DpnII 87 GATC ^GATC
1020 GATC
DraII 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
421 AGGACCT
EcoO109I 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
421 AGGACCT
MboI 87 GATC ^GATC
1020 GATC
MvnI 295 CGCG CG^CG
545 CGCG
NciI 299 CCGGG CC^[GC]GG
621 CCGGG
NdeII 87 GATC ^GATC
1020 GATC
NspI 857 ACATGT [GA]CATG^[CT]
983 GCATGT
RsaI 201 GTAC GT^AC
666 GTAC
Sau3AI 87 GATC ^GATC
1020 GATC
TfiI 31 GATTC G^A[AT]TC
149 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BbvI BmyI BpmI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboII MnlI MspA1I MspI
MvaI MwoI NlaIII NlaIV PleI
PvuII Sau96I ScrFI TseI TspRI