Restriction map for
pF1KB6439
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspMI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AccI 695 GTCTAC GT^[AC][GT]AC
AccII 16 CGCG CG^CG
AflIII 751 ACATGT A^C[GA][CT]GT
Alw44I 525 GTGCAC G^TGCAC
AocI 418 CCTCAGG CC^T[ACGT]AGG
ApaLI 525 GTGCAC G^TGCAC
Asp700I 648 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 525 GTGCAC G[AT]GC[AT]^C
AspI 689 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 688 GGACC G^G[AT]CC
BamHI 871 GGATCC G^GATCC
BanII 388 GAGCCC G[GA]GC[CT]^C
BbsI 321 GAAGAC GAAGAC(2/6)
BfaI 814 CTAG C^TAG
BpuAI 321 GAAGAC GAAGAC(2/6)
BsaAI 25 TACGTG [CT]AC^GT[GA]
BsaI 373 GAGACC GGTCTC(1/5)
BsiHKAI 525 GTGCAC G[AT]GC[AT]^C
BsiYI 638 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 638 CCATCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 687 GGGAC GGGAC(10/14)
Bsp1407I 425 TGTACA T^GTACA
BspHI 531 TCATGA T^CATGA
BspLU11I 751 ACATGT A^CATGT
BsrBI 408 CCGCTC CCGCTC(-3/-3)
BsrDI 393 CATTGC GCAATG(2/0)
BsrFI 453 GCCGGC [GA]^CCGG[CT]
BsrGI 425 TGTACA T^GTACA
BstAPI 169 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 801 GGTCACC G^GT[ACGT]ACC
BstPI 801 GGTCACC G^GT[ACGT]ACC
BstUI 16 CGCG CG^CG
BstYI 871 GGATCC [GA]^GATC[CT]
Bsu36I 418 CCTCAGG CC^T[ACGT]AGG
CfoI 15 GCGC GCG^C
Cfr10I 453 GCCGGC [GA]^CCGG[CT]
DpnII 872 GATC ^GATC
DsaI 704 CCATGG C^C[GA][CT]GG
Eco81I 418 CCTCAGG CC^T[ACGT]AGG
EcoO65I 801 GGTCACC G^GT[ACGT]ACC
FauI 407 CCCGC CCCGC(4/6)
HapII 454 CCGG C^CGG
HhaI 15 GCGC GCG^C
HinP1I 15 GCGC G^CGC
HpaII 454 CCGG C^CGG
MaeI 814 CTAG C^TAG
MboI 872 GATC ^GATC
MflI 871 GGATCC [GA]^GATC[CT]
MslI 465 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 516 CCGCTG C[AC]G^C[GT]G
MspI 454 CCGG C^CGG
MvnI 16 CGCG CG^CG
NaeI 453 GCCGGC GCC^GGC
NcoI 704 CCATGG C^CATGG
NdeII 872 GATC ^GATC
NgoMIV 453 GCCGGC G^CCGGC
PflFI 689 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 179 CTGCAG CTGCA^G
RcaI 531 TCATGA T^CATGA
SapI 269 GCTCTTC GCTCTTC(1/4)
Sau3AI 872 GATC ^GATC
SexAI 690 ACCAGGT A^CC[AT]GGT
SfcI 179 CTGCAG C^T[GA][CT]AG
SmlI 676 CTCAAG C^T[CT][GA]AG
SspBI 425 TGTACA T^GTACA
StuI 810 AGGCCT AGG^CCT
TspRI 624 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 689 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 615 CCACAATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 871 GGATCC [GA]^GATC[CT]
XmnI 648 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 157 GAATTT [GA]^AATT[CT]
510 GAATTT
AlwI 872 GATCC GGATC(4/5)
871 GGATC
ApoI 157 GAATTT [GA]^AATT[CT]
510 GAATTT
BsmAI 89 GAGAC GTCTC(1/5)
373 GAGAC
EarI 270 CTCTTC CTCTTC(1/4)
331 GAAGAG
EcoT14I 704 CCATGG C^C[AT][AT]GG
851 CCAAGG
HgaI 324 GACGC GACGC(5/10)
17 GCGTC
HinfI 108 GATTC G^A[ACGT]TC
205 GAATC
HphI 601 GGTGA GGTGA(8/7)
803 TCACC
Ksp632I 270 CTCTTC CTCTTC(1/4)
331 GAAGAG
MaeII 26 ACGT A^CGT
222 ACGT
MaeIII 755 GTGAC ^GT[ACGT]AC
802 GTCAC
MseI 260 TTAA T^TAA
551 TTAA
NlaIV 687 GGGACC GG[ACGT]^[ACGT]CC
871 GGATCC
NspI 751 ACATGT [GA]CATG^[CT]
783 GCATGT
StyI 704 CCATGG C^C[AT][AT]GG
851 CCAAGG
TaqI 191 TCGA T^CGA
656 TCGA
TfiI 108 GATTC G^A[AT]TC
205 GAATC
Tru9I 260 TTAA T^TAA
551 TTAA
Tsp45I 755 GTGAC ^GT[GC]AC
802 GTCAC
TthHB8I 191 TCGA T^CGA
656 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BmyI BsaJI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EaeI EcoRII Fnu4HI FokI HaeIII
ItaI MboII MluNI MnlI MscI
MvaI MwoI NlaIII RsaI Sau96I
ScrFI SfaNI TseI Tsp509I