##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        Tru9I       Tth111I     XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AciI          183  CCGC                 CCGC(-3/-1)
AcsI          212  GAATTC               [GA]^AATT[CT]
AfaI          349  GTAC                 GT^AC
AlwNI          61  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          212  GAATTC               [GA]^AATT[CT]
AvaII         318  GGACC                G^G[AT]CC
BcgI          266  GCAACCAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          157  CTAG                 C^TAG
BsaI           42  GAGACC               GGTCTC(1/5)
BsiYI         231  CCAAAGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          231  CCAAAGTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          42  GAGAC                GTCTC(1/5)
Bsp1407I      348  TGTACA               T^GTACA
BsrGI         348  TGTACA               T^GTACA
BssKI         315  CCTGG                ^CC[ACGT]GG
BstAPI         60  GCAGGTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         315  CCTGG                CC^[AT]GG
Cfr13I        318  GGACC                G^G[ACGT]CC
DpnII         246  GATC                 ^GATC
EcoRI         212  GAATTC               G^AATTC
EcoRII        315  CCTGG                ^CC[AT]GG
HphI          367  GGTGA                GGTGA(8/7)
MaeI          157  CTAG                 C^TAG
MboI          246  GATC                 ^GATC
MspA1I        183  CCGCTG               C[AC]G^C[GT]G
MvaI          315  CCTGG                CC^[AT]GG
MwoI           60  GCAGGTGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         246  GATC                 ^GATC
NspI          188  GCATGT               [GA]CATG^[CT]
PflMI         231  CCAAAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          349  GTAC                 GT^AC
Sau3AI        246  GATC                 ^GATC
Sau96I        318  GGACC                G^G[ACGT]CC
ScrFI         315  CCTGG                CC^[ACGT]GG
SfaNI         325  GATGC                GCATC(5/9)
SspBI         348  TGTACA               T^GTACA
TaqI          275  TCGA                 T^CGA
Tsp45I        164  GTGAC                ^GT[GC]AC
TthHB8I       275  TCGA                 T^CGA
Van91I        231  CCAAAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          156  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BspMI          60  GCAGGT               ACCTGC(4/8)
              364  GCAGGT              
BsrDI          80  CATTGC               GCAATG(2/0)
              262  CATTGC              
DdeI            7  CTCAG                C^T[ACGT]AG
              122  CTAAG               
HinfI         335  GAATC                G^A[ACGT]TC
              373  GAATC               
MaeIII         32  GTAAC                ^GT[ACGT]AC
              164  GTGAC               
NlaIII         52  CATG                 CATG^
              189  CATG                
NlaIV         256  GGAACC               GG[ACGT]^[ACGT]CC
              318  GGACCC              
TfiI          335  GAATC                G^A[AT]TC
              373  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

BbvI        BstF5I      Fnu4HI      FokI        ItaI        
MnlI        TseI        Tsp509I     TspRI