Restriction map for
pF1KE0537
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BcgI
BciVI BfrI BglI BlnI BmyI
BsaAI BsaBI BsaI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI DsaI Eam1105I EarI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
TspRI Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 568 GACGTC GACGT^C
AccII 37 CGCG CG^CG
AccIII 392 TCCGGA T^CCGGA
AcyI 568 GACGTC G[GA]^CG[CT]C
Aor51HI 593 AGCGCT AGC^GCT
BalI 708 TGGCCA TGG^CCA
BbsI 571 GTCTTC GAAGAC(2/6)
BfaI 659 CTAG C^TAG
BglII 683 AGATCT A^GATCT
BlpI 178 GCTGAGC GC^T[ACGT]AGC
BpmI 439 CTGGAG CTGGAG(16/14)
Bpu1102I 178 GCTGAGC GC^T[ACGT]AGC
BpuAI 571 GTCTTC GAAGAC(2/6)
BsaHI 568 GACGTC G[GA]^CG[CT]C
BsaWI 392 TCCGGA [AT]^CCGG[AT]
BseAI 392 TCCGGA T^CCGGA
BsiEI 423 CGGCCG CG[GA][CT]^CG
BspEI 392 TCCGGA T^CCGGA
BspHI 777 TCATGA T^CATGA
BsrFI 75 GCCGGC [GA]^CCGG[CT]
BsrI 465 ACTGG ACTGG(1/-1)
BstUI 37 CGCG CG^CG
BstXI 639 CCACTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 178 GCTGAGC GC^T[ACGT]AGC
Cfr10I 75 GCCGGC [GA]^CCGG[CT]
DraII 64 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 712 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 423 CGGCCG C^GGCCG
EclXI 423 CGGCCG C^GGCCG
Eco47III 593 AGCGCT AGC^GCT
Eco52I 423 CGGCCG C^GGCCG
EcoO109I 64 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 23 CCCGC CCCGC(4/6)
HaeII 593 AGCGCT [GA]GCGC^[CT]
HgaI 38 GCGTC GACGC(5/10)
Hin1I 568 GACGTC G[GA]^CG[CT]C
MaeI 659 CTAG C^TAG
MluNI 708 TGGCCA TGG^CCA
MroI 392 TCCGGA T^CCGGA
MscI 708 TGGCCA TGG^CCA
MspA1I 184 CAGCTG C[AC]G^C[GT]G
MvnI 37 CGCG CG^CG
NaeI 75 GCCGGC GCC^GGC
NgoMIV 75 GCCGGC G^CCGGC
NspI 401 GCATGC [GA]CATG^[CT]
PaeR7I 545 CTCGAG C^TCGAG
PflMI 711 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 64 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 184 CAGCTG CAG^CTG
RcaI 777 TCATGA T^CATGA
SfaNI 746 GATGC GCATC(5/9)
SmlI 545 CTCGAG C^T[CT][GA]AG
SphI 401 GCATGC GCATG^C
Tsp509I 90 AATT ^AATT
Van91I 711 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 545 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 483 GATCC GGATC(4/5)
449 GGATC
AocI 97 CCTGAGG CC^T[ACGT]AGG
727 CCTCAGG
AvaI 545 CTCGAG C^[CT]CG[GA]G
577 CCCGAG
AvaII 65 GGACC G^G[AT]CC
615 GGACC
BbvCI 336 CCTCAGC CCTCAGC(-5/-2)
534 GCTGAGG
BclI 323 TGATCA T^GATCA
557 TGATCA
BsoBI 545 CTCGAG C^[CT]CG[GA]G
577 CCCGAG
BstYI 482 AGATCC [GA]^GATC[CT]
683 AGATCT
Bsu36I 97 CCTGAGG CC^T[ACGT]AGG
727 CCTCAGG
CfoI 36 GCGC GCG^C
594 GCGC
Eco81I 97 CCTGAGG CC^T[ACGT]AGG
727 CCTCAGG
EcoNI 297 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
612 CCTGGACCAGG
FbaI 323 TGATCA T^GATCA
557 TGATCA
HhaI 36 GCGC GCG^C
594 GCGC
HinP1I 36 GCGC G^CGC
594 GCGC
HphI 190 GGTGA GGTGA(8/7)
8 TCACC
MaeII 56 ACGT A^CGT
569 ACGT
MaeIII 7 GTCAC ^GT[ACGT]AC
191 GTGAC
MflI 482 AGATCC [GA]^GATC[CT]
683 AGATCT
PstI 115 CTGCAG CTGCA^G
313 CTGCAG
SfcI 115 CTGCAG C^T[GA][CT]AG
313 CTGCAG
Tsp45I 7 GTCAC ^GT[GC]AC
191 GTGAC
XhoII 482 AGATCC [GA]^GATC[CT]
683 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
TseI TthHB8I