Restriction map for
pF1KB6356
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccII AccIII AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
BamHI BbvCI BcgI BciVI BfrI
BglII BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KspI MfeI
MflI MluI MroI MseI MslI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 285 AACGTT AA^CGTT
AcsI 229 GAATTC [GA]^AATT[CT]
AflIII 836 ACATGT A^C[GA][CT]GT
ApoI 229 GAATTC [GA]^AATT[CT]
AvrII 291 CCTAGG C^CTAGG
BalI 540 TGGCCA TGG^CCA
BbrPI 544 CACGTG CAC^GTG
BbsI 672 GAAGAC GAAGAC(2/6)
BclI 246 TGATCA T^GATCA
BfaI 292 CTAG C^TAG
BglI 488 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 291 CCTAGG C^CTAGG
BlpI 271 GCTGAGC GC^T[ACGT]AGC
BpmI 499 CTCCAG CTGGAG(16/14)
Bpu1102I 271 GCTGAGC GC^T[ACGT]AGC
BpuAI 672 GAAGAC GAAGAC(2/6)
BsaAI 544 CACGTG [CT]AC^GT[GA]
BsaBI 592 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 301 GAGACC GGTCTC(1/5)
BsmAI 301 GAGAC GTCTC(1/5)
BsmFI 434 GGGAC GGGAC(10/14)
BspLU11I 836 ACATGT A^CATGT
BspMI 126 ACCTGC ACCTGC(4/8)
CelII 271 GCTGAGC GC^T[ACGT]AGC
DdeI 272 CTGAG C^T[ACGT]AG
DsaI 170 CCGTGG C^C[GA][CT]GG
EarI 151 CTCTTC CTCTTC(1/4)
EcoNI 345 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 229 GAATTC G^AATTC
FbaI 246 TGATCA T^GATCA
Ksp632I 151 CTCTTC CTCTTC(1/4)
MaeI 292 CTAG C^TAG
MamI 592 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 540 TGGCCA TGG^CCA
MscI 540 TGGCCA TGG^CCA
NlaIII 837 CATG CATG^
NspI 836 ACATGT [GA]CATG^[CT]
PflMI 501 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 675 GACTC GAGTC(4/5)
PmaCI 544 CACGTG CAC^GTG
PmlI 544 CACGTG CAC^GTG
Psp1406I 285 AACGTT AA^CGTT
PstI 477 CTGCAG CTGCA^G
PvuI 597 CGATCG CGAT^CG
PvuII 480 CAGCTG CAG^CTG
TaqI 227 TCGA T^CGA
Tsp509I 230 AATT ^AATT
TthHB8I 227 TCGA T^CGA
Van91I 501 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 68 GGTACC G^GTACC
123 GGTACC
AcyI 105 GGCGCC G[GA]^CG[CT]C
394 GGCGCC
AluI 135 AGCT AG^CT
481 AGCT
AlwNI 340 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
480 CAGCTGCTG
Asp718I 68 GGTACC G^GTACC
123 GGTACC
BbeI 105 GGCGCC GGCGC^C
394 GGCGCC
BsaHI 105 GGCGCC G[GA]^CG[CT]C
394 GGCGCC
BseRI 361 GAGGAG GAGGAG(10/8)
854 GAGGAG
BsgI 466 CTGCAC GTGCAG(16/14)
238 GTGCAG
BsrFI 78 GCCGGC [GA]^CCGG[CT]
401 GCCGGC
BsrI 109 CCAGT ACTGG(1/-1)
881 CCAGT
BstAPI 368 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
479 GCAGCTGCTGC
Cfr10I 78 GCCGGC [GA]^CCGG[CT]
401 GCCGGC
DpnII 247 GATC ^GATC
598 GATC
EagI 356 CGGCCG C^GGCCG
403 CGGCCG
EclXI 356 CGGCCG C^GGCCG
403 CGGCCG
Eco52I 356 CGGCCG C^GGCCG
403 CGGCCG
EcoT14I 176 CCTTGG C^C[AT][AT]GG
291 CCTAGG
Hin1I 105 GGCGCC G[GA]^CG[CT]C
394 GGCGCC
KasI 105 GGCGCC G^GCGCC
394 GGCGCC
KpnI 68 GGTACC GGTAC^C
123 GGTACC
MaeII 286 ACGT A^CGT
545 ACGT
MaeIII 58 GTCAC ^GT[ACGT]AC
877 GTCAC
MboI 247 GATC ^GATC
598 GATC
MboII 672 GAAGA GAAGA(8/7)
152 TCTTC
NaeI 78 GCCGGC GCC^GGC
401 GCCGGC
NarI 105 GGCGCC GG^CGCC
394 GGCGCC
NdeII 247 GATC ^GATC
598 GATC
NgoMIV 78 GCCGGC G^CCGGC
401 GCCGGC
Sau3AI 247 GATC ^GATC
598 GATC
SfoI 105 GGCGCC GGC^GCC
394 GGCGCC
SmaI 97 CCCGGG CCC^GGG
430 CCCGGG
StyI 176 CCTTGG C^C[AT][AT]GG
291 CCTAGG
TfiI 258 GATTC G^A[AT]TC
685 GAATC
Tsp45I 58 GTCAC ^GT[GC]AC
877 GTCAC
XmaCI 97 CCCGGG C^CCGGG
430 CCCGGG
XmaI 97 CCCGGG C^CCGGG
430 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI ApaI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiEI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MnlI MspA1I
MspI MvaI MwoI NciI NlaIV
RsaI Sau96I ScrFI SfaNI SfcI
TseI TspRI