Restriction map for
pF1KE1081
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# NO CUT ENZYMES ( 85 enzymes) #
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Acc65I AccII AccIII AflIII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AvrII BbeI BbrPI BbvCI
BcgI BciVI BglI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BstAPI BstEII
BstPI BstUI CelII Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FauI
FseI HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AclI 2770 AACGTT AA^CGTT
AflII 1371 CTTAAG C^TTAAG
AhdI 3627 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3373 GTGCAC G^TGCAC
ApaLI 3373 GTGCAC G^TGCAC
Asp700I 2876 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3627 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2630 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4007 TGCGCA TGC^GCA
BalI 4036 TGGCCA TGG^CCA
BamHI 2704 GGATCC G^GATCC
BanI 4048 GGTGCC G^G[CT][GA]CC
BfrI 1371 CTTAAG C^TTAAG
BstBI 1685 TTCGAA TT^CGAA
DraIII 2853 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1905 GACGTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4280 CCATGG C^C[GA][CT]GG
EaeI 4036 TGGCCA [CT]^GGCC[GA]
Eam1105I 3627 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 831 GAATTC G^AATTC
EcoRV 642 GATATC GAT^ATC
FspI 4007 TGCGCA TGC^GCA
HaeII 557 AGCGCC [GA]GCGC^[CT]
HgaI 3412 GACGC GACGC(5/10)
MfeI 2984 CAATTG C^AATTG
MluNI 4036 TGGCCA TGG^CCA
MscI 4036 TGGCCA TGG^CCA
MspA1I 2750 CAGCTG C[AC]G^C[GT]G
MunI 2984 CAATTG C^AATTG
NcoI 4280 CCATGG C^CATGG
NspI 3840 GCATGT [GA]CATG^[CT]
NspV 1685 TTCGAA TT^CGAA
PaeR7I 593 CTCGAG C^TCGAG
PflFI 2630 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4404 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2677 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2770 AACGTT AA^CGTT
PvuII 2750 CAGCTG CAG^CTG
SacI 858 GAGCTC GAGCT^C
SexAI 3395 ACCAGGT A^CC[AT]GGT
SfuI 1685 TTCGAA TT^CGAA
SmaI 4400 CCCGGG CCC^GGG
SspI 220 AATATT AAT^ATT
StuI 1697 AGGCCT AGG^CCT
Tth111I 2630 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 726 TCTAGA T^CTAGA
XcmI 3115 CCATTGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 593 CTCGAG C^TCGAG
XmaCI 4400 CCCGGG C^CCGGG
XmaI 4400 CCCGGG C^CCGGG
XmnI 2876 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 298 GACGTC GACGT^C
1905 GACGTC
AccI 1803 GTATAC GT^[AC][GT]AC
3365 GTATAC
AciI 2163 GCGG CCGC(-3/-1)
2166 GCGG
AcyI 298 GACGTC G[GA]^CG[CT]C
1905 GACGTC
AlwNI 556 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
980 CAGCATCTG
AspHI 858 GAGCTC G[AT]GC[AT]^C
3373 GTGCAC
BclI 449 TGATCA T^GATCA
3723 TGATCA
BsaBI 902 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1462 GATCTTGATC
BsaHI 298 GACGTC G[GA]^CG[CT]C
1905 GACGTC
BsaI 1032 GAGACC GGTCTC(1/5)
1446 GAGACC
BsgI 1630 CTGCAC GTGCAG(16/14)
1349 GTGCAG
BsiHKAI 858 GAGCTC G[AT]GC[AT]^C
3373 GTGCAC
BspHI 71 TCATGA T^CATGA
3380 TCATGA
BspMI 1304 ACCTGC ACCTGC(4/8)
2944 ACCTGC
BsrDI 752 CATTGC GCAATG(2/0)
3116 CATTGC
BssSI 1512 CACGAG CACGAG(-5/-1)
3674 CTCGTG
Bst1107I 1803 GTATAC GTA^TAC
3365 GTATAC
BstZ17I 1803 GTATAC GTA^TAC
3365 GTATAC
DraII 4404 GGGACCC [GA]G^G[ACGT]CC[CT]
4464 AGGCCCC
EcoO109I 4404 GGGACCC [GA]G^G[ACGT]CC[CT]
4464 AGGCCCC
FbaI 449 TGATCA T^GATCA
3723 TGATCA
Hin1I 298 GACGTC G[GA]^CG[CT]C
1905 GACGTC
MamI 902 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1462 GATCTTGATC
PflMI 4028 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4039 CCATTCTTTGG
RcaI 71 TCATGA T^CATGA
3380 TCATGA
ScaI 2039 AGTACT AGT^ACT
4096 AGTACT
Van91I 4028 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4039 CCATTCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AcsI AfaI AluI AlwI AocI
ApoI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
EarI Eco81I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII