Restriction map for
pF1KB6412
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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ApaI 600 GGGCCC GGGCC^C
AvaI 525 CCCGGG C^[CT]CG[GA]G
BamHI 452 GGATCC G^GATCC
BanI 392 GGCACC G^G[CT][GA]CC
BbsI 213 GAAGAC GAAGAC(2/6)
BbvCI 922 CCTCAGC CCTCAGC(-5/-2)
BpuAI 213 GAAGAC GAAGAC(2/6)
BsiEI 146 CGATCG CG[GA][CT]^CG
BsmI 346 GAATGC GAATGC(1/-1)
BsoBI 525 CCCGGG C^[CT]CG[GA]G
BspMI 43 ACCTGC ACCTGC(4/8)
BstYI 452 GGATCC [GA]^GATC[CT]
DrdI 889 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 672 CCATGG C^C[AT][AT]GG
EcoT22I 348 ATGCAT ATGCA^T
FauI 9 CCCGC CCCGC(4/6)
HinfI 723 GAATC G^A[ACGT]TC
MflI 452 GGATCC [GA]^GATC[CT]
NaeI 389 GCCGGC GCC^GGC
NcoI 672 CCATGG C^CATGG
NgoMIV 389 GCCGGC G^CCGGC
NsiI 348 ATGCAT ATGCA^T
PflMI 974 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 146 CGATCG CGAT^CG
PvuII 651 CAGCTG CAG^CTG
SapI 172 GAAGAGC GCTCTTC(1/4)
SfaNI 677 GATGC GCATC(5/9)
SmaI 525 CCCGGG CCC^GGG
SphI 74 GCATGC GCATG^C
StyI 672 CCATGG C^C[AT][AT]GG
TaqI 344 TCGA T^CGA
TfiI 723 GAATC G^A[AT]TC
TthHB8I 344 TCGA T^CGA
Van91I 974 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 452 GGATCC [GA]^GATC[CT]
XmaCI 525 CCCGGG C^CCGGG
XmaI 525 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 473 GGCGTC G[GA]^CG[CT]C
826 GACGCC
AlwI 453 GATCC GGATC(4/5)
452 GGATC
AlwNI 397 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
427 CAGTGCCTG
BanII 528 GGGCTC G[GA]GC[CT]^C
600 GGGCCC
BmyI 528 GGGCTC G[AGT]GC[ACT]^C
600 GGGCCC
BpmI 178 CTGGAG CTGGAG(16/14)
610 CTGGAG
BsaHI 473 GGCGTC G[GA]^CG[CT]C
826 GACGCC
BsmAI 691 GAGAC GTCTC(1/5)
853 GTCTC
BsmFI 123 GGGAC GGGAC(10/14)
49 GTCCC
Bsp1286I 528 GGGCTC G[AGT]GC[ACT]^C
600 GGGCCC
BsrFI 389 GCCGGC [GA]^CCGG[CT]
742 ACCGGC
BstEII 4 GGTCACC G^GT[ACGT]ACC
441 GGTGACC
BstPI 4 GGTCACC G^GT[ACGT]ACC
441 GGTGACC
BstXI 52 CCAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
492 CCACCTCCGTGG
CfoI 116 GCGC GCG^C
153 GCGC
Cfr10I 389 GCCGGC [GA]^CCGG[CT]
742 ACCGGC
DsaI 498 CCGTGG C^C[GA][CT]GG
672 CCATGG
EaeI 309 TGGCCG [CT]^GGCC[GA]
636 TGGCCG
EcoNI 120 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
261 CCTTACCGAGG
EcoO65I 4 GGTCACC G^GT[ACGT]ACC
441 GGTGACC
HhaI 116 GCGC GCG^C
153 GCGC
Hin1I 473 GGCGTC G[GA]^CG[CT]C
826 GACGCC
HinP1I 116 GCGC G^CGC
153 GCGC
NspI 74 GCATGC [GA]CATG^[CT]
810 ACATGC
PpuMI 128 AGGACCT [GA]G^G[AT]CC[CT]
223 AGGACCC
PstI 135 CTGCAG CTGCA^G
403 CTGCAG
SfcI 135 CTGCAG C^T[GA][CT]AG
403 CTGCAG
XcmI 297 CCAGGGCAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
900 CCAGTACCGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI