Restriction map for
pF1KB6768
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MboII MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 250 AAATTT [GA]^AATT[CT]
AfaI 270 GTAC GT^AC
AlwI 102 GATCC GGATC(4/5)
ApoI 250 AAATTT [GA]^AATT[CT]
BanI 169 GGCACC G^G[CT][GA]CC
BanII 162 GGGCTC G[GA]GC[CT]^C
BclI 89 TGATCA T^GATCA
BfaI 16 CTAG C^TAG
BmyI 162 GGGCTC G[AGT]GC[ACT]^C
BsiEI 360 CGACCG CG[GA][CT]^CG
BsiYI 117 CCCCTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 117 CCCCTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 162 GGGCTC G[AGT]GC[ACT]^C
BsrI 186 ACTGG ACTGG(1/-1)
BstF5I 180 GGATG GGATG(2/0)
BstXI 230 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 15 GCTAGC GC[ACGT]^[ACGT]GC
Cfr13I 65 GGGCC G^G[ACGT]CC
DdeI 165 CTCAG C^T[ACGT]AG
DsaI 176 CCGTGG C^C[GA][CT]GG
EaeI 35 TGGCCG [CT]^GGCC[GA]
FbaI 89 TGATCA T^GATCA
FokI 180 GGATG GGATG(9/13)
HapII 106 CCGG C^CGG
HpaII 106 CCGG C^CGG
HphI 311 TCACC GGTGA(8/7)
MaeI 16 CTAG C^TAG
MaeIII 113 GTGAC ^GT[ACGT]AC
MspI 106 CCGG C^CGG
NheI 15 GCTAGC G^CTAGC
NlaIV 169 GGCACC GG[ACGT]^[ACGT]CC
PvuII 243 CAGCTG CAG^CTG
RsaI 270 GTAC GT^AC
Sau96I 65 GGGCC G^G[ACGT]CC
SfaNI 53 GCATC GCATC(5/9)
SfcI 315 CTGTAG C^T[GA][CT]AG
SmaI 105 CCCGGG CCC^GGG
StuI 341 AGGCCT AGG^CCT
Tsp45I 113 GTGAC ^GT[GC]AC
Tsp509I 251 AATT ^AATT
TspRI 32 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 105 CCCGGG C^CCGGG
XmaI 105 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 62 CTCGGG C^[CT]CG[GA]G
105 CCCGGG
BcnI 105 CCCGG CC^[GC]GG
106 CCGGG
BsoBI 62 CTCGGG C^[CT]CG[GA]G
105 CCCGGG
BstNI 225 CCAGG CC^[AT]GG
417 CCTGG
DpnII 90 GATC ^GATC
102 GATC
EcoRII 225 CCAGG ^CC[AT]GG
417 CCTGG
MboI 90 GATC ^GATC
102 GATC
MnlI 61 CCTC CCTC(7/6)
340 GAGG
MslI 171 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
287 CATACTCATG
MspA1I 48 CAGCGG C[AC]G^C[GT]G
243 CAGCTG
MvaI 225 CCAGG CC^[AT]GG
417 CCTGG
NciI 105 CCCGG CC^[GC]GG
106 CCGGG
NdeII 90 GATC ^GATC
102 GATC
NlaIII 208 CATG CATG^
293 CATG
Sau3AI 90 GATC ^GATC
102 GATC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
Fnu4HI HaeIII ItaI MwoI ScrFI
TseI