Restriction map for
pF1KB5190
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AvrII BamHI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 1117 GGCGCC G[GA]^CG[CT]C
AlwI 1572 GATCC GGATC(4/5)
AlwNI 386 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1159 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 382 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1425 GGACC G^G[AT]CC
AviII 1491 TGCGCA TGC^GCA
BalI 1526 TGGCCA TGG^CCA
BanII 539 GGGCTC G[GA]GC[CT]^C
BbeI 1117 GGCGCC GGCGC^C
BlpI 404 GCTCAGC GC^T[ACGT]AGC
BmyI 539 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 404 GCTCAGC GC^T[ACGT]AGC
BsaHI 1117 GGCGCC G[GA]^CG[CT]C
BsmBI 852 GAGACG CGTCTC(1/5)
Bsp1286I 539 GGGCTC G[AGT]GC[ACT]^C
BsrBI 1036 GAGCGG CCGCTC(-3/-3)
BssSI 1276 CACGAG CACGAG(-5/-1)
BstXI 732 CCACTCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1571 AGATCC [GA]^GATC[CT]
CelII 404 GCTCAGC GC^T[ACGT]AGC
DraI 271 TTTAAA TTT^AAA
DsaI 866 CCATGG C^C[GA][CT]GG
EcoNI 1464 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1038 GCGGG CCCGC(4/6)
FspI 1491 TGCGCA TGC^GCA
HaeII 1117 GGCGCC [GA]GCGC^[CT]
Hin1I 1117 GGCGCC G[GA]^CG[CT]C
KasI 1117 GGCGCC G^GCGCC
MaeII 528 ACGT A^CGT
MflI 1571 AGATCC [GA]^GATC[CT]
MluNI 1526 TGGCCA TGG^CCA
MscI 1526 TGGCCA TGG^CCA
MseI 272 TTAA T^TAA
MspA1I 1740 CCGCTG C[AC]G^C[GT]G
NarI 1117 GGCGCC GG^CGCC
NcoI 866 CCATGG C^CATGG
NheI 409 GCTAGC G^CTAGC
NspI 1745 GCATGT [GA]CATG^[CT]
PflFI 382 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1470 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 392 CTGCAG CTGCA^G
PvuI 40 CGATCG CGAT^CG
ScaI 691 AGTACT AGT^ACT
SfoI 1117 GGCGCC GGC^GCC
SspI 103 AATATT AAT^ATT
TfiI 1267 GATTC G^A[AT]TC
Tru9I 272 TTAA T^TAA
Tth111I 382 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1470 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1412 CCATTTCCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1571 AGATCC [GA]^GATC[CT]
XmnI 1159 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaI 784 CCCGAG C^[CT]CG[GA]G
1567 CCCGAG
BciVI 708 GGATAC GTATCC(6/5)
129 GTATCC
BsgI 524 CTGCAC GTGCAG(16/14)
584 GTGCAG
BsiEI 40 CGATCG CG[GA][CT]^CG
241 CGACCG
BsoBI 784 CCCGAG C^[CT]CG[GA]G
1567 CCCGAG
BspMI 522 ACCTGC ACCTGC(4/8)
1726 ACCTGC
BsrDI 1139 GCAATG GCAATG(2/0)
1474 GCAATG
EaeI 869 TGGCCG [CT]^GGCC[GA]
1526 TGGCCA
EcoT14I 866 CCATGG C^C[AT][AT]GG
1219 CCTTGG
SfcI 392 CTGCAG C^T[GA][CT]AG
1629 CTGTAG
StyI 866 CCATGG C^C[AT][AT]GG
1219 CCTTGG
TaqI 972 TCGA T^CGA
1167 TCGA
TthHB8I 972 TCGA T^CGA
1167 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanI BbsI BbvI BcnI BfaI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI