Restriction map for
pF1KB6375
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstUI BstXI BstZ17I CfoI
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI HinP1I HindIII HinfI
HpaI HpaII KasI KpnI KspI
MaeI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI TfiI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcsI 484 AAATTT [GA]^AATT[CT]
AcyI 653 GGCGTC G[GA]^CG[CT]C
AflIII 402 ACACGT A^C[GA][CT]GT
AocI 388 CCTGAGG CC^T[ACGT]AGG
ApaI 519 GGGCCC GGGCC^C
ApoI 484 AAATTT [GA]^AATT[CT]
Asp700I 313 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 445 GTGCTC G[AT]GC[AT]^C
BbrPI 403 CACGTG CAC^GTG
BbvCI 79 GCTGAGG CCTCAGC(-5/-2)
BlpI 447 GCTCAGC GC^T[ACGT]AGC
BpmI 382 CTGGAG CTGGAG(16/14)
Bpu1102I 447 GCTCAGC GC^T[ACGT]AGC
BsaAI 403 CACGTG [CT]AC^GT[GA]
BsaBI 46 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 653 GGCGTC G[GA]^CG[CT]C
BsiHKAI 445 GTGCTC G[AT]GC[AT]^C
BsmBI 745 GAGACG CGTCTC(1/5)
BspMI 188 ACCTGC ACCTGC(4/8)
BsrDI 426 CATTGC GCAATG(2/0)
BssSI 250 CACGAG CACGAG(-5/-1)
BstEII 585 GGTGACC G^GT[ACGT]ACC
BstPI 585 GGTGACC G^GT[ACGT]ACC
Bsu36I 388 CCTGAGG CC^T[ACGT]AGG
CelII 447 GCTCAGC GC^T[ACGT]AGC
DraI 487 TTTAAA TTT^AAA
DsaI 716 CCATGG C^C[GA][CT]GG
Eco81I 388 CCTGAGG CC^T[ACGT]AGG
EcoO65I 585 GGTGACC G^GT[ACGT]ACC
HgaI 654 GCGTC GACGC(5/10)
Hin1I 653 GGCGTC G[GA]^CG[CT]C
HincII 706 GTTGAC GT[CT]^[GA]AC
HindII 706 GTTGAC GT[CT]^[GA]AC
MaeIII 586 GTGAC ^GT[ACGT]AC
MamI 46 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 525 CAATTG C^AATTG
MslI 563 CATATATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 525 CAATTG C^AATTG
NcoI 716 CCATGG C^CATGG
PflMI 349 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 403 CACGTG CAC^GTG
PmlI 403 CACGTG CAC^GTG
SmlI 10 CTCAAG C^T[CT][GA]AG
SwaI 486 ATTTAAAT ATTT^AAAT
Tsp45I 586 GTGAC ^GT[GC]AC
TspRI 785 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 349 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 313 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbsI 313 GAAGAC GAAGAC(2/6)
472 GAAGAC
BglII 179 AGATCT A^GATCT
351 AGATCT
BpuAI 313 GAAGAC GAAGAC(2/6)
472 GAAGAC
BsmI 218 GAATGC GAATGC(1/-1)
264 GAATGC
BstBI 215 TTCGAA TT^CGAA
728 TTCGAA
BstYI 179 AGATCT [GA]^GATC[CT]
351 AGATCT
EarI 742 GAAGAG CTCTTC(1/4)
750 GAAGAG
EcoT14I 515 CCTTGG C^C[AT][AT]GG
716 CCATGG
Ksp632I 742 GAAGAG CTCTTC(1/4)
750 GAAGAG
MaeII 208 ACGT A^CGT
404 ACGT
MflI 179 AGATCT [GA]^GATC[CT]
351 AGATCT
MseI 326 TTAA T^TAA
488 TTAA
MwoI 259 GCCTGGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
645 GCAAGAGAGGC
NlaIII 306 CATG CATG^
717 CATG
NspV 215 TTCGAA TT^CGAA
728 TTCGAA
SfuI 215 TTCGAA TT^CGAA
728 TTCGAA
StyI 515 CCTTGG C^C[AT][AT]GG
716 CCATGG
Tru9I 326 TTAA T^TAA
488 TTAA
XhoII 179 AGATCT [GA]^GATC[CT]
351 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HphI ItaI MboI MboII MnlI
MspA1I MvaI NdeII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TseI Tsp509I
TthHB8I