Restriction map for
pF1KB5401
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HindIII HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 265 GGCGTC G[GA]^CG[CT]C
AocI 739 CCTCAGG CC^T[ACGT]AGG
ApaI 235 GGGCCC GGGCC^C
BanII 235 GGGCCC G[GA]GC[CT]^C
BbsI 699 GAAGAC GAAGAC(2/6)
BfaI 46 CTAG C^TAG
BmyI 235 GGGCCC G[AGT]GC[ACT]^C
BpuAI 699 GAAGAC GAAGAC(2/6)
BsaAI 511 TACGTG [CT]AC^GT[GA]
BsaHI 265 GGCGTC G[GA]^CG[CT]C
BsaI 307 GAGACC GGTCTC(1/5)
BsmAI 307 GAGAC GTCTC(1/5)
BsmI 22 GCATTC GAATGC(1/-1)
Bsp1286I 235 GGGCCC G[AGT]GC[ACT]^C
BsrDI 543 CATTGC GCAATG(2/0)
BstYI 61 GGATCT [GA]^GATC[CT]
Bsu36I 739 CCTCAGG CC^T[ACGT]AGG
EaeI 87 CGGCCA [CT]^GGCC[GA]
Eco81I 739 CCTCAGG CC^T[ACGT]AGG
EcoNI 578 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 479 CCAAGG C^C[AT][AT]GG
Hin1I 265 GGCGTC G[GA]^CG[CT]C
HincII 733 GTTGAC GT[CT]^[GA]AC
HindII 733 GTTGAC GT[CT]^[GA]AC
MaeI 46 CTAG C^TAG
MaeIII 203 GTTAC ^GT[ACGT]AC
MboII 699 GAAGA GAAGA(8/7)
MflI 61 GGATCT [GA]^GATC[CT]
NspI 38 ACATGC [GA]CATG^[CT]
PflMI 442 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 431 CAGCTG CAG^CTG
StyI 479 CCAAGG C^C[AT][AT]GG
TfiI 644 GAATC G^A[AT]TC
Van91I 442 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 61 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 16 GTAC GT^AC
96 GTAC
AluI 432 AGCT AG^CT
555 AGCT
AlwI 61 GGATC GGATC(4/5)
452 GGATC
AlwNI 201 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
319 CAGGCCCTG
BclI 536 TGATCA T^GATCA
612 TGATCA
BpmI 728 CTCCAG CTGGAG(16/14)
65 CTGGAG
BsaBI 447 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
531 GATGGTGATC
BspMI 293 ACCTGC ACCTGC(4/8)
384 GCAGGT
BsrBI 413 CCGCTC CCGCTC(-3/-3)
159 GAGCGG
BsrFI 190 GCCGGC [GA]^CCGG[CT]
262 GCCGGC
BstF5I 470 GGATG GGATG(2/0)
693 GGATG
Cfr10I 190 GCCGGC [GA]^CCGG[CT]
262 GCCGGC
DdeI 272 CTGAG C^T[ACGT]AG
740 CTCAG
FbaI 536 TGATCA T^GATCA
612 TGATCA
FokI 470 GGATG GGATG(9/13)
693 GGATG
HphI 534 GGTGA GGTGA(8/7)
668 TCACC
MaeII 248 ACGT A^CGT
512 ACGT
MamI 447 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
531 GATGGTGATC
MslI 522 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
528 CATGATGGTG
NaeI 190 GCCGGC GCC^GGC
262 GCCGGC
NgoMIV 190 GCCGGC G^CCGGC
262 GCCGGC
PleI 726 GACTC GAGTC(4/5)
777 GACTC
RsaI 16 GTAC GT^AC
96 GTAC
SfaNI 636 GATGC GCATC(5/9)
560 GCATC
TspRI 252 CAGTG CA[GC]TG[ACGT][ACGT]^
443 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AvaII BbvI BcnI
BsaJI BsgI BsiYI BslI BsmFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI MboI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI TseI