Restriction map for
pF1KB6335
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AseI AsnI AspEI AspHI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NciI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 629 GGTACC G^GTACC
AlwI 778 GGATC GGATC(4/5)
AscI 9 GGCGCGCC GG^CGCGCC
Asp700I 601 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 629 GGTACC G^GTACC
BalI 197 TGGCCA TGG^CCA
BanI 629 GGTACC G^G[CT][GA]CC
BanII 24 GGGCTC G[GA]GC[CT]^C
BciVI 288 GGATAC GTATCC(6/5)
BfaI 301 CTAG C^TAG
BglII 447 AGATCT A^GATCT
BmyI 24 GGGCTC G[AGT]GC[ACT]^C
BpmI 93 CTCCAG CTGGAG(16/14)
BsaAI 434 CACGTA [CT]AC^GT[GA]
BseRI 571 GAGGAG GAGGAG(10/8)
BsgI 142 GTGCAG GTGCAG(16/14)
BsiEI 186 CGACCG CG[GA][CT]^CG
BsmAI 133 GTCTC GTCTC(1/5)
BsmBI 132 CGTCTC CGTCTC(1/5)
Bsp1286I 24 GGGCTC G[AGT]GC[ACT]^C
BspMI 107 ACCTGC ACCTGC(4/8)
BsrDI 248 GCAATG GCAATG(2/0)
BsrFI 244 GCCGGC [GA]^CCGG[CT]
BssHII 10 GCGCGC G^CGCGC
Cfr10I 244 GCCGGC [GA]^CCGG[CT]
DraII 59 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 200 CCATGG C^C[GA][CT]GG
EaeI 197 TGGCCA [CT]^GGCC[GA]
EarI 663 CTCTTC CTCTTC(1/4)
EcoO109I 59 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 380 GAATTC G^AATTC
EcoRV 256 GATATC GAT^ATC
HaeII 297 GGCGCT [GA]GCGC^[CT]
HapII 245 CCGG C^CGG
HinfI 121 GACTC G^A[ACGT]TC
HpaII 245 CCGG C^CGG
KpnI 629 GGTACC GGTAC^C
Ksp632I 663 CTCTTC CTCTTC(1/4)
MaeI 301 CTAG C^TAG
MluNI 197 TGGCCA TGG^CCA
MscI 197 TGGCCA TGG^CCA
MspI 245 CCGG C^CGG
NaeI 244 GCCGGC GCC^GGC
NcoI 200 CCATGG C^CATGG
NgoMIV 244 GCCGGC G^CCGGC
PleI 121 GACTC GAGTC(4/5)
PpuMI 59 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 399 CAGCTG CAG^CTG
StuI 74 AGGCCT AGG^CCT
TspRI 623 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 601 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 11 CGCG CG^CG
285 CGCG
AvaII 18 GGTCC G^G[AT]CC
60 GGACC
BbvI 144 GCAGC GCAGC(8/12)
182 GCTGC
BsmFI 732 GGGAC GGGAC(10/14)
126 GTCCC
BsrI 218 CCAGT ACTGG(1/-1)
604 CCAGT
BstF5I 30 CATCC GGATG(2/0)
768 GGATG
BstUI 11 CGCG CG^CG
285 CGCG
BstYI 447 AGATCT [GA]^GATC[CT]
778 GGATCT
EcoNI 15 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
33 CCTCAAGAAGG
EcoT14I 200 CCATGG C^C[AT][AT]GG
293 CCTTGG
Fnu4HI 144 GCAGC GC^[ACGT]GC
182 GCTGC
FokI 30 CATCC GGATG(9/13)
768 GGATG
ItaI 144 GCAGC GC^[ACGT]GC
182 GCTGC
MaeII 131 ACGT A^CGT
435 ACGT
MflI 447 AGATCT [GA]^GATC[CT]
778 GGATCT
MseI 539 TTAA T^TAA
739 TTAA
MvnI 11 CGCG CG^CG
285 CGCG
MwoI 147 GCTCACCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
182 GCTGCGACCGC
NlaIII 117 CATG CATG^
201 CATG
PstI 109 CTGCAG CTGCA^G
689 CTGCAG
SfaNI 414 GATGC GCATC(5/9)
466 GATGC
SfcI 109 CTGCAG C^T[GA][CT]AG
689 CTGCAG
SmlI 34 CTCAAG C^T[CT][GA]AG
55 CTCAAG
StyI 200 CCATGG C^C[AT][AT]GG
293 CCTTGG
Tru9I 539 TTAA T^TAA
739 TTAA
TseI 144 GCAGC G^C[AT]GC
182 GCTGC
Tsp45I 546 GTCAC ^GT[GC]AC
673 GTGAC
XhoII 447 AGATCT [GA]^GATC[CT]
778 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BsaJI BsiYI BslI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII HaeIII HhaI HinP1I HphI
MaeIII MboI MboII MnlI MslI
MspA1I MvaI NdeII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI Tsp509I
TthHB8I