Restriction map for
pF1KB6751
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI Alw44I AlwI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmAI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MboI MfeI
MflI MluI MroI MunI MvnI
NarI NciI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
Sau3AI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI
SwaI TfiI Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AfaI 207 GTAC GT^AC
AflIII 203 ACGTGT A^C[GA][CT]GT
AlwNI 43 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 57 CCTGAGG CC^T[ACGT]AGG
Aor51HI 25 AGCGCT AGC^GCT
AspHI 231 GAGCAC G[AT]GC[AT]^C
BalI 275 TGGCCA TGG^CCA
BbvCI 28 GCTGAGG CCTCAGC(-5/-2)
BglI 160 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 231 GAGCAC G[AGT]GC[ACT]^C
BseRI 31 GAGGAG GAGGAG(10/8)
BsiHKAI 231 GAGCAC G[AT]GC[AT]^C
Bsp1286I 231 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 206 TGTACA T^GTACA
BspHI 146 TCATGA T^CATGA
BsrFI 247 GCCGGC [GA]^CCGG[CT]
BsrGI 206 TGTACA T^GTACA
BsrI 177 ACTGG ACTGG(1/-1)
BssKI 273 CCTGG ^CC[ACGT]GG
BstF5I 187 CATCC GGATG(2/0)
BstNI 273 CCTGG CC^[AT]GG
Bsu36I 57 CCTGAGG CC^T[ACGT]AGG
CfoI 26 GCGC GCG^C
Cfr10I 247 GCCGGC [GA]^CCGG[CT]
DraII 133 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 275 TGGCCA [CT]^GGCC[GA]
Eco47III 25 AGCGCT AGC^GCT
Eco81I 57 CCTGAGG CC^T[ACGT]AGG
EcoO109I 133 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 273 CCTGG ^CC[AT]GG
FokI 187 CATCC GGATG(9/13)
HaeII 25 AGCGCT [GA]GCGC^[CT]
HapII 248 CCGG C^CGG
HhaI 26 GCGC GCG^C
HinP1I 26 GCGC G^CGC
HpaII 248 CCGG C^CGG
MaeII 203 ACGT A^CGT
MboII 101 TCTTC GAAGA(8/7)
MluNI 275 TGGCCA TGG^CCA
MscI 275 TGGCCA TGG^CCA
MseI 200 TTAA T^TAA
MslI 238 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 43 CAGCTG C[AC]G^C[GT]G
MspI 248 CCGG C^CGG
MvaI 273 CCTGG CC^[AT]GG
NaeI 247 GCCGGC GCC^GGC
NgoMIV 247 GCCGGC G^CCGGC
NlaIV 38 GGGACC GG[ACGT]^[ACGT]CC
PstI 163 CTGCAG CTGCA^G
PvuII 43 CAGCTG CAG^CTG
RcaI 146 TCATGA T^CATGA
RsaI 207 GTAC GT^AC
SbfI 162 CCTGCAGG CCTGCA^GG
ScrFI 273 CCTGG CC^[ACGT]GG
SfaNI 94 GATGC GCATC(5/9)
SfcI 163 CTGCAG C^T[GA][CT]AG
Sse8387I 162 CCTGCAGG CCTGCA^GG
SspBI 206 TGTACA T^GTACA
TaqI 287 TCGA T^CGA
Tru9I 200 TTAA T^TAA
TspRI 299 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 287 TCGA T^CGA
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# TWO CUT ENZYMES ( 9 enzymes) #
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BbvI 45 GCTGC GCAGC(8/12)
48 GCTGC
BlpI 21 GCTGAGC GC^T[ACGT]AGC
228 GCTGAGC
Bpu1102I 21 GCTGAGC GC^T[ACGT]AGC
228 GCTGAGC
BsmFI 38 GGGAC GGGAC(10/14)
263 GGGAC
CelII 21 GCTGAGC GC^T[ACGT]AGC
228 GCTGAGC
Fnu4HI 45 GCTGC GC^[ACGT]GC
48 GCTGC
ItaI 45 GCTGC GC^[ACGT]GC
48 GCTGC
MwoI 4 GCTGGCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
160 GCCCTGCAGGC
TseI 45 GCTGC G^C[AT]GC
48 GCTGC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AluI AvaII Cac8I Cfr13I DdeI
HaeIII MnlI NlaIII Sau96I