Restriction map for
pF1KE0010
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BcnI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1085 GTCGAC GT^[AC][GT]AC
AflII 1704 CTTAAG C^TTAAG
AocI 1390 CCTCAGG CC^T[ACGT]AGG
BamHI 1194 GGATCC G^GATCC
BanI 577 GGCACC G^G[CT][GA]CC
BanII 1437 GGGCTC G[GA]GC[CT]^C
BbsI 2089 GAAGAC GAAGAC(2/6)
BclI 87 TGATCA T^GATCA
BfrI 1704 CTTAAG C^TTAAG
BglI 462 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1030 AGATCT A^GATCT
BpuAI 2089 GAAGAC GAAGAC(2/6)
BsaI 1236 GGTCTC GGTCTC(1/5)
Bsp1407I 1752 TGTACA T^GTACA
BspHI 1838 TCATGA T^CATGA
BsrDI 206 GCAATG GCAATG(2/0)
BsrFI 2255 ACCGGC [GA]^CCGG[CT]
BsrGI 1752 TGTACA T^GTACA
BssSI 2165 CACGAG CACGAG(-5/-1)
Bsu36I 1390 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2255 ACCGGC [GA]^CCGG[CT]
DsaI 2082 CCGTGG C^C[GA][CT]GG
Eco81I 1390 CCTCAGG CC^T[ACGT]AGG
EcoNI 1000 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 363 GAATTC G^AATTC
EcoT14I 520 CCAAGG C^C[AT][AT]GG
EcoT22I 50 ATGCAT ATGCA^T
FbaI 87 TGATCA T^GATCA
HaeII 152 AGCGCC [GA]GCGC^[CT]
MaeII 1623 ACGT A^CGT
NdeI 1199 CATATG CA^TATG
NsiI 50 ATGCAT ATGCA^T
NspI 1691 GCATGT [GA]CATG^[CT]
PflMI 368 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 824 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1838 TCATGA T^CATGA
SalI 1085 GTCGAC G^TCGAC
SapI 1645 GAAGAGC GCTCTTC(1/4)
SmlI 1704 CTTAAG C^T[CT][GA]AG
SspBI 1752 TGTACA T^GTACA
StuI 1732 AGGCCT AGG^CCT
StyI 520 CCAAGG C^C[AT][AT]GG
Van91I 368 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2040 CCAGAACAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 363 GAATTC [GA]^AATT[CT]
1682 AAATTT
AfaI 601 GTAC GT^AC
1753 GTAC
ApoI 363 GAATTC [GA]^AATT[CT]
1682 AAATTT
BalI 1397 TGGCCA TGG^CCA
2198 TGGCCA
BciVI 2303 GGATAC GTATCC(6/5)
1766 GTATCC
BsaWI 1053 ACCGGA [AT]^CCGG[AT]
1163 ACCGGA
BspMI 1016 ACCTGC ACCTGC(4/8)
1653 ACCTGC
BstEII 238 GGTGACC G^GT[ACGT]ACC
739 GGTCACC
BstPI 238 GGTGACC G^GT[ACGT]ACC
739 GGTCACC
CfoI 153 GCGC GCG^C
1371 GCGC
EaeI 1397 TGGCCA [CT]^GGCC[GA]
2198 TGGCCA
EcoO65I 238 GGTGACC G^GT[ACGT]ACC
739 GGTCACC
FauI 1144 GCGGG CCCGC(4/6)
1221 GCGGG
HhaI 153 GCGC GCG^C
1371 GCGC
HinP1I 153 GCGC G^CGC
1371 GCGC
HindIII 1378 AAGCTT A^AGCTT
1450 AAGCTT
MluNI 1397 TGGCCA TGG^CCA
2198 TGGCCA
MscI 1397 TGGCCA TGG^CCA
2198 TGGCCA
MseI 191 TTAA T^TAA
1705 TTAA
MslI 1833 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2028 CACTGCTGTG
PstI 711 CTGCAG CTGCA^G
2287 CTGCAG
PvuII 1331 CAGCTG CAG^CTG
1826 CAGCTG
RsaI 601 GTAC GT^AC
1753 GTAC
TaqI 1086 TCGA T^CGA
1545 TCGA
Tru9I 191 TTAA T^TAA
1705 TTAA
TthHB8I 1086 TCGA T^CGA
1545 TCGA
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
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AciI AluI AlwI AlwNI AspHI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
Tsp45I Tsp509I TspRI XhoII