##################################
# NO CUT ENZYMES (  185 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DdeI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KasI        KpnI        KspI        MaeI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NarI        NcoI        NdeI        
NdeII       NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AluI          185  AGCT                 AG^CT
Aor51HI       213  AGCGCT               AGC^GCT
ApaI          160  GGGCCC               GGGCC^C
AvaI          136  CCCGGG               C^[CT]CG[GA]G
AvaII         102  GGACC                G^G[AT]CC
AviII         113  TGCGCA               TGC^GCA
BanI          202  GGCACC               G^G[CT][GA]CC
BanII         160  GGGCCC               G[GA]GC[CT]^C
BseRI         229  GAGGAG               GAGGAG(10/8)
BsoBI         136  CCCGGG               C^[CT]CG[GA]G
BspMI         239  ACCTGC               ACCTGC(4/8)
BsrBI         279  GAGCGG               CCGCTC(-3/-3)
BsrFI           4  GCCGGC               [GA]^CCGG[CT]
BsrI          255  CCAGT                ACTGG(1/-1)
BstF5I         63  CATCC                GGATG(2/0)
Cfr10I          4  GCCGGC               [GA]^CCGG[CT]
EarI          181  GAAGAG               CTCTTC(1/4)
Eco47III      213  AGCGCT               AGC^GCT
FokI           63  CATCC                GGATG(9/13)
FseI            3  GGCCGGCC             GGCCGG^CC
FspI          113  TGCGCA               TGC^GCA
HgaI          349  GACGC                GACGC(5/10)
Ksp632I       181  GAAGAG               CTCTTC(1/4)
MaeII         110  ACGT                 A^CGT
MboII         181  GAAGA                GAAGA(8/7)
NaeI            4  GCCGGC               GCC^GGC
NgoMIV          4  GCCGGC               G^CCGGC
PstI          312  CTGCAG               CTGCA^G
SapI          181  GAAGAGC              GCTCTTC(1/4)
SfcI          312  CTGCAG               C^T[GA][CT]AG
SmaI          136  CCCGGG               CCC^GGG
TaqI          107  TCGA                 T^CGA
TthHB8I       107  TCGA                 T^CGA
XmaCI         136  CCCGGG               C^CCGGG
XmaI          136  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BmyI          160  GGGCCC               G[AGT]GC[ACT]^C
              201  GGGCAC              
BsiYI          76  CCCCTGGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              288  CCGCTGCGCGG         
BslI           76  CCCCTGGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              288  CCGCTGCGCGG         
Bsp1286I      160  GGGCCC               G[AGT]GC[ACT]^C
              201  GGGCAC              
EaeI            2  TGGCCG               [CT]^GGCC[GA]
              261  CGGCCA              
FauI          275  GCGGG                CCCGC(4/6)
              281  GCGGG               
HaeII          42  GGCGCT               [GA]GCGC^[CT]
              213  AGCGCT              
MspA1I        150  CCGCTG               C[AC]G^C[GT]G
              288  CCGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AccII       AciI        BbvI        BcnI        BglI        
BsaJI       BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NlaIV       Sau96I      ScrFI       TseI