Restriction map for
pF1KB6888
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII Bst1107I BstAPI BstEII
BstF5I BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 300 GCGG CCGC(-3/-1)
AgeI 81 ACCGGT A^CCGGT
AluI 483 AGCT AG^CT
AocI 363 CCTCAGG CC^T[ACGT]AGG
AviII 497 TGCGCA TGC^GCA
BanI 84 GGTGCC G^G[CT][GA]CC
BbvI 564 GCAGC GCAGC(8/12)
BfaI 76 CTAG C^TAG
BmyI 85 GTGCCC G[AGT]GC[ACT]^C
BsaAI 325 TACGTG [CT]AC^GT[GA]
BsmAI 448 GAGAC GTCTC(1/5)
BsmBI 448 GAGACG CGTCTC(1/5)
BsmI 500 GCATTC GAATGC(1/-1)
Bsp1286I 85 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 521 TGTACA T^GTACA
BsrFI 81 ACCGGT [GA]^CCGG[CT]
BsrGI 521 TGTACA T^GTACA
BssSI 568 CACGAG CACGAG(-5/-1)
BstBI 149 TTCGAA TT^CGAA
BstXI 320 CCAGATACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 363 CCTCAGG CC^T[ACGT]AGG
Cfr10I 81 ACCGGT [GA]^CCGG[CT]
Eco81I 363 CCTCAGG CC^T[ACGT]AGG
EcoT14I 455 CCAAGG C^C[AT][AT]GG
FauI 300 GCGGG CCCGC(4/6)
Fnu4HI 564 GCAGC GC^[ACGT]GC
FspI 497 TGCGCA TGC^GCA
HaeII 3 GGCGCT [GA]GCGC^[CT]
HinfI 598 GAATC G^A[ACGT]TC
ItaI 564 GCAGC GC^[ACGT]GC
MaeI 76 CTAG C^TAG
MboII 411 GAAGA GAAGA(8/7)
MspA1I 298 CAGCGG C[AC]G^C[GT]G
NlaIII 384 CATG CATG^
NspI 383 ACATGC [GA]CATG^[CT]
NspV 149 TTCGAA TT^CGAA
PinAI 81 ACCGGT A^CCGGT
SfuI 149 TTCGAA TT^CGAA
SspBI 521 TGTACA T^GTACA
StyI 455 CCAAGG C^C[AT][AT]GG
TfiI 598 GAATC G^A[AT]TC
TseI 564 GCAGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BpmI 318 CTCCAG CTGGAG(16/14)
556 CTGGAG
BsaWI 81 ACCGGT [AT]^CCGG[AT]
110 ACCGGA
BseRI 157 GAGGAG GAGGAG(10/8)
589 GAGGAG
BsiYI 75 CCTAGTACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
104 CCCTGGACCGG
BslI 75 CCTAGTACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
104 CCCTGGACCGG
BsmFI 310 GGGAC GGGAC(10/14)
368 GGGAC
BssKI 11 CCTGG ^CC[ACGT]GG
105 CCTGG
BstNI 11 CCTGG CC^[AT]GG
105 CCTGG
BstYI 56 AGATCC [GA]^GATC[CT]
182 AGATCC
Cac8I 387 GCTTGC GC[ACGT]^[ACGT]GC
494 GCTTGC
CfoI 4 GCGC GCG^C
498 GCGC
EcoRII 11 CCTGG ^CC[AT]GG
105 CCTGG
HaeIII 144 GGCC GG^CC
334 GGCC
HapII 82 CCGG C^CGG
111 CCGG
HhaI 4 GCGC GCG^C
498 GCGC
HinP1I 4 GCGC G^CGC
498 GCGC
HpaII 82 CCGG C^CGG
111 CCGG
HphI 92 TCACC GGTGA(8/7)
206 TCACC
MaeII 326 ACGT A^CGT
398 ACGT
MflI 56 AGATCC [GA]^GATC[CT]
182 AGATCC
MspI 82 CCGG C^CGG
111 CCGG
MvaI 11 CCTGG CC^[AT]GG
105 CCTGG
NlaIV 84 GGTGCC GG[ACGT]^[ACGT]CC
310 GGGACC
ScrFI 11 CCTGG CC^[ACGT]GG
105 CCTGG
SfaNI 139 GCATC GCATC(5/9)
269 GCATC
TaqI 150 TCGA T^CGA
488 TCGA
TthHB8I 150 TCGA T^CGA
488 TCGA
XhoII 56 AGATCC [GA]^GATC[CT]
182 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AfaI AlwI AvaII BsaJI BsrI
Cfr13I DdeI DpnII MboI MnlI
NdeII RsaI Sau3AI Sau96I TspRI
XcmI