Restriction map for
pF1KE0104
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BsaAI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HindIII
KasI KpnI KspI MaeII MluI
MroI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI TfiI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1215 GTCTAC GT^[AC][GT]AC
AcsI 715 GAATTC [GA]^AATT[CT]
AcyI 934 GGCGTC G[GA]^CG[CT]C
ApoI 715 GAATTC [GA]^AATT[CT]
AseI 1350 ATTAAT AT^TAAT
AsnI 1350 ATTAAT AT^TAAT
AvaI 648 CCCGAG C^[CT]CG[GA]G
BanI 170 GGCACC G^G[CT][GA]CC
BglI 1010 GCCTTTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 814 AGATCT A^GATCT
BlpI 1199 GCTTAGC GC^T[ACGT]AGC
BmyI 169 GGGCAC G[AGT]GC[ACT]^C
BpmI 1076 CTGGAG CTGGAG(16/14)
Bpu1102I 1199 GCTTAGC GC^T[ACGT]AGC
BsaBI 463 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 934 GGCGTC G[GA]^CG[CT]C
BsaWI 380 ACCGGA [AT]^CCGG[AT]
BseRI 655 GAGGAG GAGGAG(10/8)
BsgI 242 CTGCAC GTGCAG(16/14)
BsmI 1312 GCATTC GAATGC(1/-1)
BsoBI 648 CCCGAG C^[CT]CG[GA]G
Bsp1286I 169 GGGCAC G[AGT]GC[ACT]^C
CelII 1199 GCTTAGC GC^T[ACGT]AGC
DraII 1277 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1165 CCATGG C^C[GA][CT]GG
EcoO109I 1277 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 715 GAATTC G^AATTC
FauI 1256 CCCGC CCCGC(4/6)
HapII 381 CCGG C^CGG
HgaI 935 GCGTC GACGC(5/10)
Hin1I 934 GGCGTC G[GA]^CG[CT]C
HinfI 1127 GAGTC G^A[ACGT]TC
HpaI 81 GTTAAC GTT^AAC
HpaII 381 CCGG C^CGG
MamI 463 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 991 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 381 CCGG C^CGG
NcoI 1165 CCATGG C^CATGG
NheI 417 GCTAGC G^CTAGC
NspI 1043 GCATGT [GA]CATG^[CT]
PflMI 853 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1127 GAGTC GAGTC(4/5)
PpuMI 1277 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1350 ATTAAT AT^TAAT
SfcI 1217 CTACAG C^T[GA][CT]AG
SwaI 464 ATTTAAAT ATTT^AAAT
TaqI 837 TCGA T^CGA
Tsp45I 209 GTGAC ^GT[GC]AC
TthHB8I 837 TCGA T^CGA
Van91I 853 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 1257 CCGC CCGC(-3/-1)
157 GCGG
AlwI 697 GATCC GGATC(4/5)
964 GATCC
AlwNI 56 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1276 CAGGTCCTG
BalI 1137 TGGCCA TGG^CCA
1168 TGGCCA
BbsI 913 GAAGAC GAAGAC(2/6)
3 GTCTTC
BpuAI 913 GAAGAC GAAGAC(2/6)
3 GTCTTC
BsiYI 648 CCCGAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
853 CCAACCTGTGG
BslI 648 CCCGAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
853 CCAACCTGTGG
BsmFI 1000 GGGAC GGGAC(10/14)
646 GTCCC
BstYI 696 AGATCC [GA]^GATC[CT]
814 AGATCT
DraI 465 TTTAAA TTT^AAA
906 TTTAAA
EaeI 1137 TGGCCA [CT]^GGCC[GA]
1168 TGGCCA
EarI 110 CTCTTC CTCTTC(1/4)
1114 GAAGAG
HphI 640 GGTGA GGTGA(8/7)
581 TCACC
Ksp632I 110 CTCTTC CTCTTC(1/4)
1114 GAAGAG
MfeI 218 CAATTG C^AATTG
775 CAATTG
MflI 696 AGATCC [GA]^GATC[CT]
814 AGATCT
MluNI 1137 TGGCCA TGG^CCA
1168 TGGCCA
MscI 1137 TGGCCA TGG^CCA
1168 TGGCCA
MunI 218 CAATTG C^AATTG
775 CAATTG
PvuII 56 CAGCTG CAG^CTG
532 CAGCTG
SfaNI 1093 GATGC GCATC(5/9)
1252 GATGC
SmlI 196 CTTGAG C^T[CT][GA]AG
806 CTCAAG
XhoII 696 AGATCC [GA]^GATC[CT]
814 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AvaII BbvI BfaI BsaJI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI StyI Tru9I TseI
Tsp509I TspRI