##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 488 GGCGCC G[GA]^CG[CT]C
AfaI 216 GTAC GT^AC
BalI 23 TGGCCA TGG^CCA
BbeI 488 GGCGCC GGCGC^C
BsaHI 488 GGCGCC G[GA]^CG[CT]C
BsaI 411 GGTCTC GGTCTC(1/5)
BssHII 570 GCGCGC G^CGCGC
BstXI 14 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 637 GATC ^GATC
Hin1I 488 GGCGCC G[GA]^CG[CT]C
HinfI 270 GAGTC G^A[ACGT]TC
HphI 148 TCACC GGTGA(8/7)
KasI 488 GGCGCC G^GCGCC
KspI 507 CCGCGG CCGC^GG
MaeII 374 ACGT A^CGT
MaeIII 656 GTGAC ^GT[ACGT]AC
MboI 637 GATC ^GATC
MluNI 23 TGGCCA TGG^CCA
MscI 23 TGGCCA TGG^CCA
NarI 488 GGCGCC GG^CGCC
NdeII 637 GATC ^GATC
PaeR7I 669 CTCGAG C^TCGAG
PleI 270 GAGTC GAGTC(4/5)
PpuMI 689 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 216 GTAC GT^AC
SacII 507 CCGCGG CCGC^GG
Sau3AI 637 GATC ^GATC
SfoI 488 GGCGCC GGC^GCC
SmlI 669 CTCGAG C^T[CT][GA]AG
Tsp45I 656 GTGAC ^GT[GC]AC
XhoI 669 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 238 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
311 CAGTCCCTG
ApaI 306 GGGCCC GGGCC^C
325 GGGCCC
AvaII 683 GGTCC G^G[AT]CC
690 GGACC
BanI 190 GGCACC G^G[CT][GA]CC
488 GGCGCC
BcgI 416 CGATGTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
519 GCAGGCCTGTCG
BpmI 205 CTGGAG CTGGAG(16/14)
559 CTGGAG
BseRI 208 GAGGAG GAGGAG(10/8)
355 GAGGAG
BsmAI 358 GAGAC GTCTC(1/5)
412 GTCTC
BsmFI 689 GGGAC GGGAC(10/14)
313 GTCCC
BsrBI 388 GAGCGG CCGCTC(-3/-3)
672 GAGCGG
BsrFI 437 ACCGGC [GA]^CCGG[CT]
659 ACCGGC
Cfr10I 437 ACCGGC [GA]^CCGG[CT]
659 ACCGGC
EarI 703 CTCTTC CTCTTC(1/4)
277 GAAGAG
HgaI 400 GACGC GACGC(5/10)
595 GACGC
Ksp632I 703 CTCTTC CTCTTC(1/4)
277 GAAGAG
SmaI 626 CCCGGG CCC^GGG
679 CCCGGG
StuI 201 AGGCCT AGG^CCT
521 AGGCCT
TaqI 415 TCGA T^CGA
670 TCGA
TthHB8I 415 TCGA T^CGA
670 TCGA
XmaCI 626 CCCGGG C^CCGGG
679 CCCGGG
XmaI 626 CCCGGG C^CCGGG
679 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BglI BmyI BsaJI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NlaIII NlaIV PstI PvuII
Sau96I ScrFI SfcI SfiI StyI
TseI TspRI