Restriction map for
pF1KB4465
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AspEI AvrII BamHI BfrI
BglII BlnI BseAI BsiWI Bsp1407I
BspEI BspHI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CpoI DraI
Eam1105I EcoO65I EcoRI EcoRV FseI
HpaI MfeI MluI MroI MunI
NdeI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SalI SfuI SnaBI SpeI SphI
SspBI SspI StuI SwaI XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2297 GACGTC GACGT^C
Acc65I 865 GGTACC G^GTACC
AocI 5029 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4196 AGCGCT AGC^GCT
AseI 3475 ATTAAT AT^TAAT
AsnI 3475 ATTAAT AT^TAAT
Asp700I 2829 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 865 GGTACC G^GTACC
AviII 4616 TGCGCA TGC^GCA
BciVI 3586 GGATAC GTATCC(6/5)
BclI 4791 TGATCA T^GATCA
BsaBI 5667 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1107 TCCGGT [AT]^CCGG[AT]
BspDI 943 ATCGAT AT^CGAT
BspLU11I 3317 ACATGT A^CATGT
BsrDI 4278 GCAATG GCAATG(2/0)
BstYI 360 AGATCC [GA]^GATC[CT]
Bsu36I 5029 CCTGAGG CC^T[ACGT]AGG
ClaI 943 ATCGAT AT^CGAT
Eco47III 4196 AGCGCT AGC^GCT
Eco81I 5029 CCTGAGG CC^T[ACGT]AGG
EcoNI 6897 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3408 ATGCAT ATGCA^T
FbaI 4791 TGATCA T^GATCA
FspI 4616 TGCGCA TGC^GCA
HindIII 3108 AAGCTT A^AGCTT
KpnI 865 GGTACC GGTAC^C
MamI 5667 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 360 AGATCC [GA]^GATC[CT]
MseI 3476 TTAA T^TAA
NotI 4018 GCGGCCGC GC^GGCCGC
NsiI 3408 ATGCAT ATGCA^T
PaeR7I 2965 CTCGAG C^TCGAG
PshBI 3475 ATTAAT AT^TAAT
SacI 5770 GAGCTC GAGCT^C
ScaI 4418 AGTACT AGT^ACT
SgrAI 4377 CGCCGGTG C[GA]^CCGG[CT]G
Tru9I 3476 TTAA T^TAA
Tsp509I 694 AATT ^AATT
XhoI 2965 CTCGAG C^TCGAG
XhoII 360 AGATCC [GA]^GATC[CT]
XmnI 2829 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflIII 1507 ACGTGT A^C[GA][CT]GT
3317 ACATGT
AspI 1456 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1924 GACTGTGTC
BbvCI 369 CCTCAGC CCTCAGC(-5/-2)
5221 GCTGAGG
BsmBI 6603 CGTCTC CGTCTC(1/5)
2204 GAGACG
BsmI 1528 GAATGC GAATGC(1/-1)
1753 GAATGC
NheI 96 GCTAGC G^CTAGC
5452 GCTAGC
PflFI 1456 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1924 GACTGTGTC
SapI 5767 GAAGAGC GCTCTTC(1/4)
6183 GAAGAGC
SexAI 1796 ACCTGGT A^CC[AT]GGT
3551 ACCTGGT
SfiI 4515 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6218 GGCCGCAGGGGCC
SmlI 2965 CTCGAG C^T[CT][GA]AG
6367 CTTGAG
Tth111I 1456 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1924 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbsI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI SacII Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmaI Sse8387I StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XmaCI XmaI