Restriction map for
pF1KB6861
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DpnII DraI DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
Ksp632I KspI MaeI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tth111I
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 215 GGTACC G^GTACC
AcsI 208 GAATTC [GA]^AATT[CT]
AcyI 12 GGCGTC G[GA]^CG[CT]C
AfaI 216 GTAC GT^AC
AlwNI 184 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 208 GAATTC [GA]^AATT[CT]
Asp718I 215 GGTACC G^GTACC
BanII 134 GAGCCC G[GA]GC[CT]^C
BciVI 342 GTATCC GTATCC(6/5)
BsaHI 12 GGCGTC G[GA]^CG[CT]C
BsmAI 101 GAGAC GTCTC(1/5)
BsrBI 526 GAGCGG CCGCTC(-3/-3)
BsrDI 337 GCAATG GCAATG(2/0)
BsrI 317 ACTGG ACTGG(1/-1)
BstXI 493 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 63 GCGGGC GC[ACGT]^[ACGT]GC
CfoI 67 GCGC GCG^C
DraIII 434 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 208 GAATTC G^AATTC
FauI 63 GCGGG CCCGC(4/6)
HaeII 66 GGCGCT [GA]GCGC^[CT]
HhaI 67 GCGC GCG^C
Hin1I 12 GGCGTC G[GA]^CG[CT]C
HinP1I 67 GCGC G^CGC
HindIII 455 AAGCTT A^AGCTT
HphI 481 GGTGA GGTGA(8/7)
KpnI 215 GGTACC GGTAC^C
MaeIII 511 GTGAC ^GT[ACGT]AC
NlaIII 534 CATG CATG^
PaeR7I 79 CTCGAG C^TCGAG
PflMI 433 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 418 CTGCAG CTGCA^G
RsaI 216 GTAC GT^AC
SexAI 435 ACCTGGT A^CC[AT]GGT
SfcI 418 CTGCAG C^T[GA][CT]AG
SmaI 521 CCCGGG CCC^GGG
SmlI 79 CTCGAG C^T[CT][GA]AG
TaqI 80 TCGA T^CGA
Tsp45I 511 GTGAC ^GT[GC]AC
Tsp509I 209 AATT ^AATT
TthHB8I 80 TCGA T^CGA
Van91I 433 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 79 CTCGAG C^TCGAG
XmaCI 521 CCCGGG C^CCGGG
XmaI 521 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 79 CTCGAG C^[CT]CG[GA]G
521 CCCGGG
BanI 215 GGTACC G^G[CT][GA]CC
262 GGCACC
BmyI 134 GAGCCC G[AGT]GC[ACT]^C
261 GGGCAC
BsmFI 18 GGGAC GGGAC(10/14)
295 GTCCC
BsoBI 79 CTCGAG C^[CT]CG[GA]G
521 CCCGGG
Bsp1286I 134 GAGCCC G[AGT]GC[ACT]^C
261 GGGCAC
BstF5I 226 CATCC GGATG(2/0)
322 CATCC
BstNI 256 CCTGG CC^[AT]GG
436 CCTGG
DdeI 309 CTTAG C^T[ACGT]AG
329 CTGAG
DraII 18 GGGACCC [GA]G^G[ACGT]CC[CT]
293 AGGTCCC
EcoO109I 18 GGGACCC [GA]G^G[ACGT]CC[CT]
293 AGGTCCC
EcoRII 256 CCTGG ^CC[AT]GG
436 CCTGG
FokI 226 CATCC GGATG(9/13)
322 CATCC
HapII 138 CCGG C^CGG
522 CCGG
HgaI 13 GCGTC GACGC(5/10)
92 GCGTC
HinfI 82 GAGTC G^A[ACGT]TC
250 GACTC
HpaII 138 CCGG C^CGG
522 CCGG
MseI 410 TTAA T^TAA
448 TTAA
MslI 151 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
494 CACTGTGGTG
MspA1I 184 CAGCTG C[AC]G^C[GT]G
415 CAGCTG
MspI 138 CCGG C^CGG
522 CCGG
MvaI 256 CCTGG CC^[AT]GG
436 CCTGG
PleI 250 GACTC GAGTC(4/5)
82 GAGTC
PpuMI 18 GGGACCC [GA]G^G[AT]CC[CT]
293 AGGTCCC
PvuII 184 CAGCTG CAG^CTG
415 CAGCTG
SfaNI 225 GCATC GCATC(5/9)
321 GCATC
Tru9I 410 TTAA T^TAA
448 TTAA
TspRI 370 CAGTG CA[GC]TG[ACGT][ACGT]^
494 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsiYI BslI BssKI Cfr13I
Fnu4HI HaeIII ItaI MboII MnlI
MwoI NciI NlaIV Sau96I ScrFI
TseI