##################################
# NO CUT ENZYMES ( 191 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeIII
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MscI MslI MspA1I
MunI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 254 TCCGGA T^CCGGA
AcyI 11 GGCGTC G[GA]^CG[CT]C
BbvCI 124 GCTGAGG CCTCAGC(-5/-2)
BsaHI 11 GGCGTC G[GA]^CG[CT]C
BsaI 103 GGTCTC GGTCTC(1/5)
BsaWI 254 TCCGGA [AT]^CCGG[AT]
BseAI 254 TCCGGA T^CCGGA
BsmAI 104 GTCTC GTCTC(1/5)
BspEI 254 TCCGGA T^CCGGA
BspMI 48 GCAGGT ACCTGC(4/8)
BssKI 308 CCTGG ^CC[ACGT]GG
BstF5I 211 GGATG GGATG(2/0)
BstNI 308 CCTGG CC^[AT]GG
DdeI 125 CTGAG C^T[ACGT]AG
DraI 274 TTTAAA TTT^AAA
DraII 50 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 50 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 308 CCTGG ^CC[AT]GG
EcoT14I 99 CCTTGG C^C[AT][AT]GG
FokI 211 GGATG GGATG(9/13)
HaeII 121 GGCGCT [GA]GCGC^[CT]
HapII 255 CCGG C^CGG
HgaI 12 GCGTC GACGC(5/10)
Hin1I 11 GGCGTC G[GA]^CG[CT]C
HpaII 255 CCGG C^CGG
MboII 298 GAAGA GAAGA(8/7)
MroI 254 TCCGGA T^CCGGA
MseI 275 TTAA T^TAA
MspI 255 CCGG C^CGG
MvaI 308 CCTGG CC^[AT]GG
PpuMI 50 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 131 CTGCAG CTGCA^G
ScrFI 308 CCTGG CC^[ACGT]GG
StyI 99 CCTTGG C^C[AT][AT]GG
Tru9I 275 TTAA T^TAA
Tsp45I 14 GTCAC ^GT[GC]AC
Tsp509I 284 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 37 CGCG CG^CG
77 CGCG
AluI 65 AGCT AG^CT
135 AGCT
AvaI 54 CCCGAG C^[CT]CG[GA]G
106 CTCGGG
AvaII 51 GGTCC G^G[AT]CC
168 GGACC
BsaJI 55 CCGAGG C^C[ACGT][ACGT]GG
99 CCTTGG
BsoBI 54 CCCGAG C^[CT]CG[GA]G
106 CTCGGG
BstUI 37 CGCG CG^CG
77 CGCG
Cac8I 8 GCTGGC GC[ACGT]^[ACGT]GC
32 GCGGGC
Cfr13I 51 GGTCC G^G[ACGT]CC
168 GGACC
HphI 144 GGTGA GGTGA(8/7)
15 TCACC
MaeIII 14 GTCAC ^GT[ACGT]AC
304 GTTAC
MvnI 37 CGCG CG^CG
77 CGCG
NlaIII 141 CATG CATG^
181 CATG
NlaIV 51 GGTCCC GG[ACGT]^[ACGT]CC
167 GGGACC
Sau96I 51 GGTCC G^G[ACGT]CC
168 GGACC
SfcI 116 CTGTAG C^T[GA][CT]AG
131 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BbvI BsmFI BsrI CfoI
FauI Fnu4HI HhaI HinP1I ItaI
MnlI MwoI TseI TspRI