Restriction map for
pF1KA1693
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspMI BsrDI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstYI BstZ17I CfoI
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MslI MunI
MvnI NarI NciI NdeI NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 2223 ACATGT A^C[GA][CT]GT
AlwI 1812 GGATC GGATC(4/5)
AlwNI 776 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BsaAI 2524 TACGTG [CT]AC^GT[GA]
Bsp1407I 1569 TGTACA T^GTACA
BspHI 741 TCATGA T^CATGA
BspLU11I 2223 ACATGT A^CATGT
BsrGI 1569 TGTACA T^GTACA
BstBI 1446 TTCGAA TT^CGAA
BstXI 2184 CCAGCCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2566 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1948 CCTGACTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2207 ATGCAT ATGCA^T
NcoI 1455 CCATGG C^CATGG
NsiI 2207 ATGCAT ATGCA^T
NspI 2223 ACATGT [GA]CATG^[CT]
NspV 1446 TTCGAA TT^CGAA
PflMI 775 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1642 CTGCAG CTGCA^G
RcaI 741 TCATGA T^CATGA
SalI 1519 GTCGAC G^TCGAC
SapI 2386 GAAGAGC GCTCTTC(1/4)
ScaI 543 AGTACT AGT^ACT
SexAI 2567 ACCTGGT A^CC[AT]GGT
SfuI 1446 TTCGAA TT^CGAA
SspBI 1569 TGTACA T^GTACA
Van91I 775 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 396 TCCGGA T^CCGGA
1209 TCCGGA
AciI 360 CCGC CCGC(-3/-1)
1173 CCGC
AocI 598 CCTGAGG CC^T[ACGT]AGG
1411 CCTGAGG
Asp700I 514 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1327 GAAGATGTTC
AspHI 271 GAGCTC G[AT]GC[AT]^C
1084 GAGCTC
AspI 700 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1513 GACAAAGTC
BalI 152 TGGCCA TGG^CCA
965 TGGCCA
BanII 271 GAGCTC G[GA]GC[CT]^C
1084 GAGCTC
BfaI 52 CTAG C^TAG
865 CTAG
BmyI 271 GAGCTC G[AGT]GC[ACT]^C
1084 GAGCTC
BsaWI 396 TCCGGA [AT]^CCGG[AT]
1209 TCCGGA
BseAI 396 TCCGGA T^CCGGA
1209 TCCGGA
BsgI 571 GTGCAG GTGCAG(16/14)
1357 GTGCAG
BsiHKAI 271 GAGCTC G[AT]GC[AT]^C
1084 GAGCTC
BsmAI 1629 GTCTC GTCTC(1/5)
2561 GTCTC
Bsp1286I 271 GAGCTC G[AGT]GC[ACT]^C
1084 GAGCTC
BspEI 396 TCCGGA T^CCGGA
1209 TCCGGA
BsrBI 360 CCGCTC CCGCTC(-3/-3)
1173 CCGCTC
BsrFI 13 GCCGGC [GA]^CCGG[CT]
826 GCCGGC
Bsu36I 598 CCTGAGG CC^T[ACGT]AGG
1411 CCTGAGG
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
826 GCCGGC
DsaI 642 CCACGG C^C[GA][CT]GG
1455 CCATGG
EaeI 152 TGGCCA [CT]^GGCC[GA]
965 TGGCCA
Eco81I 598 CCTGAGG CC^T[ACGT]AGG
1411 CCTGAGG
FauI 359 CCCGC CCCGC(4/6)
1172 CCCGC
HindIII 241 AAGCTT A^AGCTT
1054 AAGCTT
HphI 2553 GGTGA GGTGA(8/7)
686 TCACC
MaeI 52 CTAG C^TAG
865 CTAG
MluNI 152 TGGCCA TGG^CCA
965 TGGCCA
MroI 396 TCCGGA T^CCGGA
1209 TCCGGA
MscI 152 TGGCCA TGG^CCA
965 TGGCCA
NaeI 13 GCCGGC GCC^GGC
826 GCCGGC
NgoMIV 13 GCCGGC G^CCGGC
826 GCCGGC
NlaIV 784 GGCCCC GG[ACGT]^[ACGT]CC
1846 GGTCCC
PflFI 700 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1513 GACAAAGTC
SacI 271 GAGCTC GAGCT^C
1084 GAGCTC
SmlI 256 CTCAAG C^T[CT][GA]AG
1069 CTCAAG
Tsp45I 653 GTGAC ^GT[GC]AC
2554 GTGAC
Tth111I 700 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1513 GACAAAGTC
XbaI 51 TCTAGA T^CTAGA
864 TCTAGA
XmnI 514 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1327 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccI AfaI AluI AvaII BbsI
BbvCI BbvI BlpI BpmI Bpu1102I
BpuAI BsaJI BseRI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I CelII Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I