Restriction map for
pF1KB7834
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AviII AvrII BbeI BbrPI BbsI
BcgI BfrI BlnI BpuAI BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HgaI HincII HindII
HindIII HpaI KasI KpnI KspI
MluI MroI MslI NarI NdeI
NheI NotI NruI NsiI PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 998 GACGTC GACGT^C
AccII 5 CGCG CG^CG
AcyI 998 GACGTC G[GA]^CG[CT]C
AflIII 467 ACATGT A^C[GA][CT]GT
AhdI 231 GACCCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 231 GACCCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1570 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1704 GGATCC G^GATCC
BanI 663 GGCACC G^G[CT][GA]CC
BanII 1225 GAGCCC G[GA]GC[CT]^C
BbvCI 134 CCTCAGC CCTCAGC(-5/-2)
BciVI 1331 GTATCC GTATCC(6/5)
BclI 357 TGATCA T^GATCA
BglI 1666 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 144 GCTAAGC GC^T[ACGT]AGC
BpmI 426 CTCCAG CTGGAG(16/14)
Bpu1102I 144 GCTAAGC GC^T[ACGT]AGC
BsaBI 1524 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 998 GACGTC G[GA]^CG[CT]C
BspHI 360 TCATGA T^CATGA
BspLU11I 467 ACATGT A^CATGT
BspMI 1499 ACCTGC ACCTGC(4/8)
BsrBI 129 CCGCTC CCGCTC(-3/-3)
BsrDI 456 CATTGC GCAATG(2/0)
BsrFI 1055 GCCGGC [GA]^CCGG[CT]
BstBI 176 TTCGAA TT^CGAA
BstUI 5 CGCG CG^CG
CelII 144 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1055 GCCGGC [GA]^CCGG[CT]
DraII 1643 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1088 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1700 CCATGG C^C[GA][CT]GG
Eam1105I 231 GACCCGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1643 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 128 CCCGC CCCGC(4/6)
FbaI 357 TGATCA T^GATCA
HaeII 512 AGCGCC [GA]GCGC^[CT]
Hin1I 998 GACGTC G[GA]^CG[CT]C
MamI 1524 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 926 CAATTG C^AATTG
MunI 926 CAATTG C^AATTG
MvnI 5 CGCG CG^CG
NaeI 1055 GCCGGC GCC^GGC
NcoI 1700 CCATGG C^CATGG
NgoMIV 1055 GCCGGC G^CCGGC
NspI 467 ACATGT [GA]CATG^[CT]
NspV 176 TTCGAA TT^CGAA
PflFI 1570 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1643 AGGACCT [GA]G^G[AT]CC[CT]
PstI 184 CTGCAG CTGCA^G
RcaI 360 TCATGA T^CATGA
ScaI 1485 AGTACT AGT^ACT
SfuI 176 TTCGAA TT^CGAA
SmaI 854 CCCGGG CCC^GGG
Tth111I 1570 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 854 CCCGGG C^CCGGG
XmaI 854 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 1350 GTAC GT^AC
1486 GTAC
Asp700I 1302 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1483 GAAGTACTTC
AspHI 73 GAGCAC G[AT]GC[AT]^C
845 GAGCAC
BalI 1064 TGGCCA TGG^CCA
1181 TGGCCA
BcnI 854 CCCGG CC^[GC]GG
855 CCGGG
BfaI 577 CTAG C^TAG
1627 CTAG
BseRI 132 CTCCTC GAGGAG(10/8)
202 GAGGAG
BsiHKAI 73 GAGCAC G[AT]GC[AT]^C
845 GAGCAC
BsmFI 996 GGGAC GGGAC(10/14)
471 GTCCC
BsmI 18 GAATGC GAATGC(1/-1)
655 GAATGC
CfoI 397 GCGC GCG^C
513 GCGC
DraI 1023 TTTAAA TTT^AAA
1632 TTTAAA
EcoNI 134 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
556 CCTACACCAGG
EcoRI 1015 GAATTC G^AATTC
1302 GAATTC
HapII 855 CCGG C^CGG
1056 CCGG
HhaI 397 GCGC GCG^C
513 GCGC
HinP1I 397 GCGC G^CGC
513 GCGC
HpaII 855 CCGG C^CGG
1056 CCGG
MaeI 577 CTAG C^TAG
1627 CTAG
MluNI 1064 TGGCCA TGG^CCA
1181 TGGCCA
MscI 1064 TGGCCA TGG^CCA
1181 TGGCCA
MspI 855 CCGG C^CGG
1056 CCGG
MwoI 506 GCCCCAAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1666 GCCAAAGAGGC
NciI 854 CCCGG CC^[GC]GG
855 CCGGG
PaeR7I 70 CTCGAG C^TCGAG
119 CTCGAG
RsaI 1350 GTAC GT^AC
1486 GTAC
SapI 842 GAAGAGC GCTCTTC(1/4)
1035 GAAGAGC
SexAI 955 ACCTGGT A^CC[AT]GGT
1089 ACCTGGT
SfcI 62 CTACAG C^T[GA][CT]AG
184 CTGCAG
XcmI 1184 CCAAACTTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1691 CCAATAAAACCATGG
XhoI 70 CTCGAG C^TCGAG
119 CTCGAG
XmnI 1302 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1483 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaI AvaII BbvI BglII
BmyI BsaJI BsiYI BslI BsmAI
BsmBI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI NdeII NlaIII NlaIV
PleI PvuII Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII