##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HindIII     HinfI       KasI        KpnI        
Ksp632I     KspI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MroI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tsp45I      Tsp509I     TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcyI           27  GGCGTC               G[GA]^CG[CT]C
AvaI          148  CCCGAG               C^[CT]CG[GA]G
AvaII          48  GGACC                G^G[AT]CC
BalI          428  TGGCCA               TGG^CCA
BcnI          348  CCGGG                CC^[GC]GG
BpmI          268  CTGGAG               CTGGAG(16/14)
BsaHI          27  GGCGTC               G[GA]^CG[CT]C
BsmAI         162  GAGAC                GTCTC(1/5)
BsoBI         148  CCCGAG               C^[CT]CG[GA]G
BspHI         222  TCATGA               T^CATGA
BsrDI         383  GCAATG               GCAATG(2/0)
BsrI          205  ACTGG                ACTGG(1/-1)
BstXI         380  CCAGCAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          121  GCGC                 GCG^C
Cfr13I         48  GGACC                G^G[ACGT]CC
DdeI          172  CTTAG                C^T[ACGT]AG
DraII          47  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoNI         462  CCTGATGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       47  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        10  CCTTGG               C^C[AT][AT]GG
HapII         348  CCGG                 C^CGG
HgaI           28  GCGTC                GACGC(5/10)
HhaI          121  GCGC                 GCG^C
Hin1I          27  GGCGTC               G[GA]^CG[CT]C
HinP1I        121  GCGC                 G^CGC
HincII        286  GTTAAC               GT[CT]^[GA]AC
HindII        286  GTTAAC               GT[CT]^[GA]AC
HpaI          286  GTTAAC               GTT^AAC
HpaII         348  CCGG                 C^CGG
HphI          366  GGTGA                GGTGA(8/7)
MaeII          89  ACGT                 A^CGT
MboII         498  TCTTC                GAAGA(8/7)
MluNI         428  TGGCCA               TGG^CCA
MscI          428  TGGCCA               TGG^CCA
MseI          287  TTAA                 T^TAA
MspA1I         35  CAGCTG               C[AC]G^C[GT]G
MspI          348  CCGG                 C^CGG
NciI          348  CCGGG                CC^[GC]GG
NlaIII        223  CATG                 CATG^
PpuMI          47  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          124  CTGCAG               CTGCA^G
PvuII          35  CAGCTG               CAG^CTG
RcaI          222  TCATGA               T^CATGA
Sau96I         48  GGACC                G^G[ACGT]CC
SfcI          124  CTGCAG               C^T[GA][CT]AG
SpeI           64  ACTAGT               A^CTAGT
StyI           10  CCTTGG               C^C[AT][AT]GG
TaqI          293  TCGA                 T^CGA
Tru9I         287  TTAA                 T^TAA
TthHB8I       293  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AluI           36  AGCT                 AG^CT
              279  AGCT                
BfaI           52  CTAG                 C^TAG
               65  CTAG                
BsaJI          10  CCTTGG               C^C[ACGT][ACGT]GG
               41  CCAGGG              
BsmFI          47  GGGAC                GGGAC(10/14)
              410  GGGAC               
Cac8I         379  GCCAGC               GC[ACGT]^[ACGT]GC
              468  GCAGGC              
MaeI           52  CTAG                 C^TAG
               65  CTAG                


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        BbvI        BsiYI       BslI        BspMI       
BssKI       BstNI       EaeI        EcoRII      Fnu4HI      
HaeIII      ItaI        MnlI        MvaI        MwoI        
NlaIV       ScrFI       SfaNI       TseI