Restriction map for
pF1KA1512
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# NO CUT ENZYMES ( 51 enzymes) #
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AatII AclI AcsI AgeI ApoI
AscI AseI AsnI Asp700I BbrPI
BsiWI Bsp1407I BspDI BspHI BsrGI
Bst1107I BstZ17I ClaI CpoI DraI
EcoRI EcoRV FseI HindIII MfeI
MluI MunI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 2309 GGTACC G^GTACC
AccI 988 GTCTAC GT^[AC][GT]AC
AccIII 2252 TCCGGA T^CCGGA
AflII 5656 CTTAAG C^TTAAG
AflIII 1610 ACATGT A^C[GA][CT]GT
AhdI 631 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2309 GGTACC G^GTACC
AspEI 631 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1149 CCTAGG C^CTAGG
BciVI 2315 GTATCC GTATCC(6/5)
BfrI 5656 CTTAAG C^TTAAG
BglII 1532 AGATCT A^GATCT
BlnI 1149 CCTAGG C^CTAGG
BsaBI 2250 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2252 TCCGGA T^CCGGA
BsiEI 2741 CGGCCG CG[GA][CT]^CG
BspEI 2252 TCCGGA T^CCGGA
BspLU11I 1610 ACATGT A^CATGT
BssHII 3200 GCGCGC G^CGCGC
BstBI 1552 TTCGAA TT^CGAA
BstXI 960 CCAGTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4138 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3330 GACGCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2741 CGGCCG C^GGCCG
Eam1105I 631 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2741 CGGCCG C^GGCCG
Eco52I 2741 CGGCCG C^GGCCG
EcoT22I 1746 ATGCAT ATGCA^T
HincII 679 GTTAAC GT[CT]^[GA]AC
HindII 679 GTTAAC GT[CT]^[GA]AC
HpaI 679 GTTAAC GTT^AAC
KpnI 2309 GGTACC GGTAC^C
MamI 2250 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2252 TCCGGA T^CCGGA
NsiI 1746 ATGCAT ATGCA^T
NspV 1552 TTCGAA TT^CGAA
ScaI 701 AGTACT AGT^ACT
SfuI 1552 TTCGAA TT^CGAA
SgrAI 2962 CGCCGGCG C[GA]^CCGG[CT]G
SphI 5735 GCATGC GCATG^C
TfiI 568 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AocI 840 CCTGAGG CC^T[ACGT]AGG
4902 CCTGAGG
Aor51HI 443 AGCGCT AGC^GCT
2855 AGCGCT
BbeI 1195 GGCGCC GGCGC^C
4094 GGCGCC
BcgI 3834 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4272 CGAAGCAGATGC
BsaAI 256 TACGTG [CT]AC^GT[GA]
1363 TACGTG
BsrDI 2231 GCAATG GCAATG(2/0)
4970 GCAATG
Bsu36I 840 CCTGAGG CC^T[ACGT]AGG
4902 CCTGAGG
Eco47III 443 AGCGCT AGC^GCT
2855 AGCGCT
Eco81I 840 CCTGAGG CC^T[ACGT]AGG
4902 CCTGAGG
KasI 1195 GGCGCC G^GCGCC
4094 GGCGCC
KspI 914 CCGCGG CCGC^GG
3617 CCGCGG
MseI 680 TTAA T^TAA
5657 TTAA
NaeI 2963 GCCGGC GCC^GGC
5240 GCCGGC
NarI 1195 GGCGCC GG^CGCC
4094 GGCGCC
NgoMIV 2963 GCCGGC G^CCGGC
5240 GCCGGC
PshAI 1947 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2772 GACGCACGTC
SacII 914 CCGCGG CCGC^GG
3617 CCGCGG
SexAI 4688 ACCAGGT A^CC[AT]GGT
5744 ACCTGGT
SfoI 1195 GGCGCC GGC^GCC
4094 GGCGCC
Tru9I 680 TTAA T^TAA
5657 TTAA
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# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AspI AvaI AvaII AviII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfiI SmaI
SmlI Sse8387I StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI