Restriction map for
pF1KB8850
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# NO CUT ENZYMES ( 210 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DdeI
DpnII DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MamI MboI MfeI MflI
MluI MroI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 17 CCGC CCGC(-3/-1)
AflII 55 CTTAAG C^TTAAG
Asp700I 192 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2 TGGCCA TGG^CCA
BbsI 139 GAAGAC GAAGAC(2/6)
BfrI 55 CTTAAG C^TTAAG
BpuAI 139 GAAGAC GAAGAC(2/6)
BssKI 35 CCAGG ^CC[ACGT]GG
BstF5I 207 CATCC GGATG(2/0)
BstNI 35 CCAGG CC^[AT]GG
BstXI 35 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoRII 35 CCAGG ^CC[AT]GG
FokI 207 CATCC GGATG(9/13)
HapII 163 CCGG C^CGG
HpaII 163 CCGG C^CGG
MluNI 2 TGGCCA TGG^CCA
MnlI 147 CCTC CCTC(7/6)
MscI 2 TGGCCA TGG^CCA
MseI 56 TTAA T^TAA
MspA1I 98 CAGCTG C[AC]G^C[GT]G
MspI 163 CCGG C^CGG
MvaI 35 CCAGG CC^[AT]GG
NlaIII 130 CATG CATG^
NspI 129 ACATGC [GA]CATG^[CT]
PstI 101 CTGCAG CTGCA^G
PvuII 98 CAGCTG CAG^CTG
ScrFI 35 CCAGG CC^[ACGT]GG
SfcI 101 CTGCAG C^T[GA][CT]AG
SmlI 55 CTTAAG C^T[CT][GA]AG
TaqI 74 TCGA T^CGA
Tru9I 56 TTAA T^TAA
TthHB8I 74 TCGA T^CGA
XmnI 192 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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BsaJI 92 CCAAGG C^C[ACGT][ACGT]GG
134 CCAAGG
BsiYI 156 CCCTGTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
177 CCCGTTTAGGG
BslI 156 CCCTGTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
177 CCCGTTTAGGG
Cac8I 4 GCCAGC GC[ACGT]^[ACGT]GC
19 GCCAGC
EcoT14I 92 CCAAGG C^C[AT][AT]GG
134 CCAAGG
HaeIII 3 GGCC GG^CC
114 GGCC
MboII 139 GAAGA GAAGA(8/7)
189 GAAGA
MwoI 19 GCCAGCATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
42 GCTGGTAGAGC
StyI 92 CCAAGG C^C[AT][AT]GG
134 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
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AluI BbvI Fnu4HI ItaI TseI