Restriction map for pF1KA0136
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BcnI        BglI        
BglII       BlnI        BmyI        BpmI        BsaHI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BsrBI       BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstEII      BstPI       BstXI       BstYI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraII       DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoT14I     EcoT22I     FseI        FspI        HaeII       
Hin1I       KasI        KpnI        MfeI        MflI        
MluI        MroI        MunI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RsrII       SacI        
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AflII        1054  CTTAAG               C^TTAAG
AflIII       2373  ACATGT               A^C[GA][CT]GT
AlwNI          95  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          664  CCCGAG               C^[CT]CG[GA]G
BbvCI          30  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2055  CGATAAATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1054  CTTAAG               C^TTAAG
BlpI          240  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      240  GCTAAGC              GC^T[ACGT]AGC
BsaAI         803  TACGTG               [CT]AC^GT[GA]
BsaWI        1434  ACCGGA               [AT]^CCGG[AT]
BseRI        2635  GAGGAG               GAGGAG(10/8)
BsoBI         664  CCCGAG               C^[CT]CG[GA]G
BspHI        1773  TCATGA               T^CATGA
BspLU11I     2373  ACATGT               A^CATGT
BspMI        1761  ACCTGC               ACCTGC(4/8)
BssHII         35  GCGCGC               G^CGCGC
BstAPI       2065  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         834  TTCGAA               TT^CGAA
CelII         240  GCTAAGC              GC^T[ACGT]AGC
DraIII       2546  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI         659  GAATTC               G^AATTC
EcoRV         652  GATATC               GAT^ATC
HapII        1435  CCGG                 C^CGG
HincII       2689  GTTAAC               GT[CT]^[GA]AC
HindII       2689  GTTAAC               GT[CT]^[GA]AC
HpaI         2689  GTTAAC               GTT^AAC
HpaII        1435  CCGG                 C^CGG
KspI           18  CCGCGG               CCGC^GG
MspI         1435  CCGG                 C^CGG
NspV          834  TTCGAA               TT^CGAA
PvuII         826  CAGCTG               CAG^CTG
RcaI         1773  TCATGA               T^CATGA
SacII          18  CCGCGG               CCGC^GG
ScaI         2812  AGTACT               AGT^ACT
SfuI          834  TTCGAA               TT^CGAA
SmlI         1054  CTTAAG               C^T[CT][GA]AG
SpeI           70  ACTAGT               A^CTAGT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          19  CGCG                 CG^CG
               36  CGCG                
AocI          750  CCTGAGG              CC^T[ACGT]AGG
             1510  CCTAAGG             
AspI          259  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2144  GACAGTGTC           
AvaII         720  GGACC                G^G[AT]CC
             2490  GGACC               
BalI           82  TGGCCA               TGG^CCA
             1857  TGGCCA              
BciVI          23  GGATAC               GTATCC(6/5)
             2189  GGATAC              
BclI         1218  TGATCA               T^GATCA
             2163  TGATCA              
BsaBI         862  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2725  GATCTTGATC          
BsgI           92  GTGCAG               GTGCAG(16/14)
             2440  GTGCAG              
BsiYI          13  CCACCCCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              197  CCGACAATGGG         
BslI           13  CCACCCCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              197  CCGACAATGGG         
BsmAI        1629  GAGAC                GTCTC(1/5)
              390  GTCTC               
BstUI          19  CGCG                 CG^CG
               36  CGCG                
Bsu36I        750  CCTGAGG              CC^T[ACGT]AGG
             1510  CCTAAGG             
DsaI           18  CCGCGG               C^C[GA][CT]GG
              527  CCACGG              
EarI         2681  CTCTTC               CTCTTC(1/4)
              837  GAAGAG              
Eco81I        750  CCTGAGG              CC^T[ACGT]AGG
             1510  CCTAAGG             
FauI           17  CCCGC                CCCGC(4/6)
               20  GCGGG               
FbaI         1218  TGATCA               T^GATCA
             2163  TGATCA              
Ksp632I      2681  CTCTTC               CTCTTC(1/4)
              837  GAAGAG              
MamI          862  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2725  GATCTTGATC          
MluNI          82  TGGCCA               TGG^CCA
             1857  TGGCCA              
MscI           82  TGGCCA               TGG^CCA
             1857  TGGCCA              
MspA1I         18  CCGCGG               C[AC]G^C[GT]G
              826  CAGCTG              
MvnI           19  CGCG                 CG^CG
               36  CGCG                
NlaIV        1341  GGTTCC               GG[ACGT]^[ACGT]CC
             2489  GGGACC              
NspI          423  GCATGT               [GA]CATG^[CT]
             2373  ACATGT              
PflFI         259  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2144  GACAGTGTC           
Tth111I       259  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2144  GACAGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        Asp700I     BbsI        
BbvI        BfaI        BpuAI       BsaJI       BsmFI       
BsmI        BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EcoRII      Fnu4HI      FokI        HaeIII      
HgaI        HhaI        HinP1I      HindIII     HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MvaI        MwoI        NdeI        NdeII       NlaIII      
PleI        PshBI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XmnI        
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