################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BamHI BanI BanII BbeI BbrPI BcnI BglI BglII BlnI BmyI BpmI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstEII BstPI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoT14I EcoT22I FseI FspI HaeII Hin1I KasI KpnI MfeI MflI MluI MroI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflII 1054 CTTAAG C^TTAAG AflIII 2373 ACATGT A^C[GA][CT]GT AlwNI 95 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 664 CCCGAG C^[CT]CG[GA]G BbvCI 30 CCTCAGC CCTCAGC(-5/-2) BcgI 2055 CGATAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1054 CTTAAG C^TTAAG BlpI 240 GCTAAGC GC^T[ACGT]AGC Bpu1102I 240 GCTAAGC GC^T[ACGT]AGC BsaAI 803 TACGTG [CT]AC^GT[GA] BsaWI 1434 ACCGGA [AT]^CCGG[AT] BseRI 2635 GAGGAG GAGGAG(10/8) BsoBI 664 CCCGAG C^[CT]CG[GA]G BspHI 1773 TCATGA T^CATGA BspLU11I 2373 ACATGT A^CATGT BspMI 1761 ACCTGC ACCTGC(4/8) BssHII 35 GCGCGC G^CGCGC BstAPI 2065 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 834 TTCGAA TT^CGAA CelII 240 GCTAAGC GC^T[ACGT]AGC DraIII 2546 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 659 GAATTC G^AATTC EcoRV 652 GATATC GAT^ATC HapII 1435 CCGG C^CGG HincII 2689 GTTAAC GT[CT]^[GA]AC HindII 2689 GTTAAC GT[CT]^[GA]AC HpaI 2689 GTTAAC GTT^AAC HpaII 1435 CCGG C^CGG KspI 18 CCGCGG CCGC^GG MspI 1435 CCGG C^CGG NspV 834 TTCGAA TT^CGAA PvuII 826 CAGCTG CAG^CTG RcaI 1773 TCATGA T^CATGA SacII 18 CCGCGG CCGC^GG ScaI 2812 AGTACT AGT^ACT SfuI 834 TTCGAA TT^CGAA SmlI 1054 CTTAAG C^T[CT][GA]AG SpeI 70 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 19 CGCG CG^CG 36 CGCG AocI 750 CCTGAGG CC^T[ACGT]AGG 1510 CCTAAGG AspI 259 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2144 GACAGTGTC AvaII 720 GGACC G^G[AT]CC 2490 GGACC BalI 82 TGGCCA TGG^CCA 1857 TGGCCA BciVI 23 GGATAC GTATCC(6/5) 2189 GGATAC BclI 1218 TGATCA T^GATCA 2163 TGATCA BsaBI 862 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2725 GATCTTGATC BsgI 92 GTGCAG GTGCAG(16/14) 2440 GTGCAG BsiYI 13 CCACCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 197 CCGACAATGGG BslI 13 CCACCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 197 CCGACAATGGG BsmAI 1629 GAGAC GTCTC(1/5) 390 GTCTC BstUI 19 CGCG CG^CG 36 CGCG Bsu36I 750 CCTGAGG CC^T[ACGT]AGG 1510 CCTAAGG DsaI 18 CCGCGG C^C[GA][CT]GG 527 CCACGG EarI 2681 CTCTTC CTCTTC(1/4) 837 GAAGAG Eco81I 750 CCTGAGG CC^T[ACGT]AGG 1510 CCTAAGG FauI 17 CCCGC CCCGC(4/6) 20 GCGGG FbaI 1218 TGATCA T^GATCA 2163 TGATCA Ksp632I 2681 CTCTTC CTCTTC(1/4) 837 GAAGAG MamI 862 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2725 GATCTTGATC MluNI 82 TGGCCA TGG^CCA 1857 TGGCCA MscI 82 TGGCCA TGG^CCA 1857 TGGCCA MspA1I 18 CCGCGG C[AC]G^C[GT]G 826 CAGCTG MvnI 19 CGCG CG^CG 36 CGCG NlaIV 1341 GGTTCC GG[ACGT]^[ACGT]CC 2489 GGGACC NspI 423 GCATGT [GA]CATG^[CT] 2373 ACATGT PflFI 259 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2144 GACAGTGTC Tth111I 259 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2144 GACAGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I BbsI BbvI BfaI BpuAI BsaJI BsmFI BsmI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HgaI HhaI HinP1I HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeI NdeII NlaIII PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI