##################################
# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
ItaI KasI KpnI Ksp632I KspI
MaeIII MamI MluI MluNI MroI
MscI MspA1I MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AfaI 241 GTAC GT^AC
AluI 336 AGCT AG^CT
AlwI 367 GGATC GGATC(4/5)
AspI 230 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 276 GGACC G^G[AT]CC
BfaI 338 CTAG C^TAG
BglII 174 AGATCT A^GATCT
BseRI 53 GAGGAG GAGGAG(10/8)
BsgI 59 GTGCAG GTGCAG(16/14)
BsmAI 87 GAGAC GTCTC(1/5)
BsrDI 214 GCAATG GCAATG(2/0)
BssKI 293 CCAGG ^CC[ACGT]GG
BstBI 269 TTCGAA TT^CGAA
BstF5I 132 GGATG GGATG(2/0)
BstNI 293 CCAGG CC^[AT]GG
Cfr13I 276 GGACC G^G[ACGT]CC
DdeI 152 CTGAG C^T[ACGT]AG
DraII 275 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 275 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 293 CCAGG ^CC[AT]GG
EcoRV 253 GATATC GAT^ATC
EcoT14I 280 CCAAGG C^C[AT][AT]GG
FokI 132 GGATG GGATG(9/13)
MaeI 338 CTAG C^TAG
MaeII 25 ACGT A^CGT
MfeI 221 CAATTG C^AATTG
MseI 299 TTAA T^TAA
MslI 195 CACTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 221 CAATTG C^AATTG
MvaI 293 CCAGG CC^[AT]GG
MwoI 337 GCTAGAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 276 GGACCC GG[ACGT]^[ACGT]CC
NspV 269 TTCGAA TT^CGAA
PflFI 230 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 275 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 241 GTAC GT^AC
Sau96I 276 GGACC G^G[ACGT]CC
ScrFI 293 CCAGG CC^[ACGT]GG
SfuI 269 TTCGAA TT^CGAA
StyI 280 CCAAGG C^C[AT][AT]GG
Tru9I 299 TTAA T^TAA
Tth111I 230 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BsaJI 280 CCAAGG C^C[ACGT][ACGT]GG
293 CCAGGG
BstYI 174 AGATCT [GA]^GATC[CT]
367 GGATCT
DpnII 175 GATC ^GATC
368 GATC
MboI 175 GATC ^GATC
368 GATC
MflI 174 AGATCT [GA]^GATC[CT]
367 GGATCT
NdeII 175 GATC ^GATC
368 GATC
PleI 204 GACTC GAGTC(4/5)
154 GAGTC
Sau3AI 175 GATC ^GATC
368 GATC
TaqI 237 TCGA T^CGA
270 TCGA
TfiI 141 GAATC G^A[AT]TC
267 GATTC
TspRI 158 CAGTG CA[GC]TG[ACGT][ACGT]^
195 CACTG
TthHB8I 237 TCGA T^CGA
270 TCGA
XhoII 174 AGATCT [GA]^GATC[CT]
367 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
BbsI BpuAI HinfI MboII MnlI
Tsp509I