Restriction map for
pF1KB8622
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AflII AgeI ApaI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BbrPI BfaI BfrI BlnI
BsmBI BsmI BspHI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DraI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI MaeI MfeI
MluI MunI NarI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SalI
SapI SbfI SfoI SfuI SgrAI
SpeI SphI Sse8387I SspI SwaI
TfiI XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 421 GTCTAC GT^[AC][GT]AC
AccIII 1717 TCCGGA T^CCGGA
AclI 2282 AACGTT AA^CGTT
AhdI 1837 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 924 GTGCAC G^TGCAC
Aor51HI 2084 AGCGCT AGC^GCT
ApaLI 924 GTGCAC G^TGCAC
Asp700I 817 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1837 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2153 GGATCC G^GATCC
BanI 1606 GGCACC G^G[CT][GA]CC
BbvCI 1827 GCTGAGG CCTCAGC(-5/-2)
BcgI 1968 CGACTACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1621 GGATAC GTATCC(6/5)
BglII 1704 AGATCT A^GATCT
BsaAI 2494 TACGTA [CT]AC^GT[GA]
BseAI 1717 TCCGGA T^CCGGA
BsiWI 2492 CGTACG C^GTACG
BspDI 34 ATCGAT AT^CGAT
BspEI 1717 TCCGGA T^CCGGA
BspLU11I 1625 ACATGT A^CATGT
ClaI 34 ATCGAT AT^CGAT
DraIII 61 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1837 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2084 AGCGCT AGC^GCT
HincII 1237 GTCAAC GT[CT]^[GA]AC
HindII 1237 GTCAAC GT[CT]^[GA]AC
HinfI 1843 GAGTC G^A[ACGT]TC
KspI 329 CCGCGG CCGC^GG
MroI 1717 TCCGGA T^CCGGA
NaeI 1946 GCCGGC GCC^GGC
NcoI 1761 CCATGG C^CATGG
NgoMIV 1946 GCCGGC G^CCGGC
NotI 20 GCGGCCGC GC^GGCCGC
NspI 1625 ACATGT [GA]CATG^[CT]
PleI 1843 GAGTC GAGTC(4/5)
Psp1406I 2282 AACGTT AA^CGTT
SacI 1318 GAGCTC GAGCT^C
SacII 329 CCGCGG CCGC^GG
SexAI 1728 ACCAGGT A^CC[AT]GGT
SfiI 2289 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2494 TACGTA TAC^GTA
StuI 379 AGGCCT AGG^CCT
Tsp45I 2402 GTGAC ^GT[GC]AC
XmnI 817 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AatII 418 GACGTC GACGT^C
1381 GACGTC
AocI 747 CCTGAGG CC^T[ACGT]AGG
1599 CCTCAGG
BclI 725 TGATCA T^GATCA
2057 TGATCA
BlpI 678 GCTGAGC GC^T[ACGT]AGC
2080 GCTGAGC
Bpu1102I 678 GCTGAGC GC^T[ACGT]AGC
2080 GCTGAGC
BsaBI 1095 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2154 GATCCACATC
BsaWI 1226 TCCGGT [AT]^CCGG[AT]
1717 TCCGGA
Bsp1407I 1097 TGTACA T^GTACA
1553 TGTACA
BsrBI 1721 GAGCGG CCGCTC(-3/-3)
2065 GAGCGG
BsrFI 1946 GCCGGC [GA]^CCGG[CT]
2306 GCCGGT
BsrGI 1097 TGTACA T^GTACA
1553 TGTACA
BstXI 548 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1736 CCAAGATGGTGG
Bsu36I 747 CCTGAGG CC^T[ACGT]AGG
1599 CCTCAGG
CelII 678 GCTGAGC GC^T[ACGT]AGC
2080 GCTGAGC
Cfr10I 1946 GCCGGC [GA]^CCGG[CT]
2306 GCCGGT
DrdI 412 GACGATGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1276 GACAAGGTGGTC
Eco81I 747 CCTGAGG CC^T[ACGT]AGG
1599 CCTCAGG
EcoT14I 794 CCAAGG C^C[AT][AT]GG
1761 CCATGG
FbaI 725 TGATCA T^GATCA
2057 TGATCA
HaeII 105 GGCGCT [GA]GCGC^[CT]
2084 AGCGCT
MaeIII 1997 GTAAC ^GT[ACGT]AC
2402 GTGAC
MamI 1095 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2154 GATCCACATC
PflMI 60 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
879 CCAGCTCCTGG
PstI 843 CTGCAG CTGCA^G
1700 CTGCAG
SspBI 1097 TGTACA T^GTACA
1553 TGTACA
StyI 794 CCAAGG C^C[AT][AT]GG
1761 CCATGG
Van91I 60 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
879 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PpuMI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmaI SmlI TaqI Tru9I TseI
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII XmaCI XmaI