Restriction map for
pF1KE0443
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsgI BsiWI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsaI SacII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Alw44I 97 GTGCAC G^TGCAC
AocI 895 CCTGAGG CC^T[ACGT]AGG
ApaI 436 GGGCCC GGGCC^C
ApaLI 97 GTGCAC G^TGCAC
BalI 653 TGGCCA TGG^CCA
BlpI 246 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 246 GCTGAGC GC^T[ACGT]AGC
BsaAI 688 TACGTG [CT]AC^GT[GA]
BseRI 898 GAGGAG GAGGAG(10/8)
BsiEI 757 CGACCG CG[GA][CT]^CG
BsmBI 369 GAGACG CGTCTC(1/5)
BsmI 677 GCATTC GAATGC(1/-1)
BsrFI 730 ACCGGC [GA]^CCGG[CT]
BssSI 223 CTCGTG CACGAG(-5/-1)
Bsu36I 895 CCTGAGG CC^T[ACGT]AGG
CelII 246 GCTGAGC GC^T[ACGT]AGC
Cfr10I 730 ACCGGC [GA]^CCGG[CT]
CpoI 359 CGGTCCG CG^G[AT]CCG
Eco81I 895 CCTGAGG CC^T[ACGT]AGG
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
MluNI 653 TGGCCA TGG^CCA
MscI 653 TGGCCA TGG^CCA
MseI 782 TTAA T^TAA
MslI 126 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 457 CTCGAG C^TCGAG
PleI 212 GACTC GAGTC(4/5)
PshAI 715 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 359 CGGTCCG CG^G[AT]CCG
SacI 901 GAGCTC GAGCT^C
SfaNI 866 GCATC GCATC(5/9)
SfiI 105 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 323 CCCGGG CCC^GGG
SmlI 457 CTCGAG C^T[CT][GA]AG
StuI 104 AGGCCT AGG^CCT
TfiI 822 GAATC G^A[AT]TC
Tru9I 782 TTAA T^TAA
XcmI 149 CCATAGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 457 CTCGAG C^TCGAG
XmaCI 323 CCCGGG C^CCGGG
XmaI 323 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 791 TCCGGA T^CCGGA
905 TCCGGA
AcyI 139 GGCGCC G[GA]^CG[CT]C
196 GGCGCC
AvaI 323 CCCGGG C^[CT]CG[GA]G
457 CTCGAG
BbeI 139 GGCGCC GGCGC^C
196 GGCGCC
BfaI 46 CTAG C^TAG
949 CTAG
BglI 106 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
322 GCCCGGGAGGC
BpmI 7 CTGGAG CTGGAG(16/14)
724 CTGGAG
BsaHI 139 GGCGCC G[GA]^CG[CT]C
196 GGCGCC
BsaI 460 GAGACC GGTCTC(1/5)
727 GAGACC
BsaWI 791 TCCGGA [AT]^CCGG[AT]
905 TCCGGA
BseAI 791 TCCGGA T^CCGGA
905 TCCGGA
BsoBI 323 CCCGGG C^[CT]CG[GA]G
457 CTCGAG
BspEI 791 TCCGGA T^CCGGA
905 TCCGGA
BsrI 859 ACTGG ACTGG(1/-1)
879 ACTGG
BstF5I 117 CATCC GGATG(2/0)
939 GGATG
BstXI 149 CCATAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
629 CCAACAAGGTGG
BstYI 41 AGATCC [GA]^GATC[CT]
518 AGATCC
DraII 435 AGGGCCC [GA]G^G[ACGT]CC[CT]
436 GGGCCCC
EaeI 146 CGGCCA [CT]^GGCC[GA]
653 TGGCCA
EcoO109I 435 AGGGCCC [GA]G^G[ACGT]CC[CT]
436 GGGCCCC
EcoT14I 560 CCATGG C^C[AT][AT]GG
672 CCATGG
FokI 117 CATCC GGATG(9/13)
939 GGATG
Hin1I 139 GGCGCC G[GA]^CG[CT]C
196 GGCGCC
HinfI 212 GACTC G^A[ACGT]TC
822 GAATC
KasI 139 GGCGCC G^GCGCC
196 GGCGCC
MaeI 46 CTAG C^TAG
949 CTAG
MaeII 372 ACGT A^CGT
689 ACGT
MflI 41 AGATCC [GA]^GATC[CT]
518 AGATCC
MroI 791 TCCGGA T^CCGGA
905 TCCGGA
NarI 139 GGCGCC GG^CGCC
196 GGCGCC
NcoI 560 CCATGG C^CATGG
672 CCATGG
PstI 37 CTGCAG CTGCA^G
187 CTGCAG
SfcI 37 CTGCAG C^T[GA][CT]AG
187 CTGCAG
SfoI 139 GGCGCC GGC^GCC
196 GGCGCC
StyI 560 CCATGG C^C[AT][AT]GG
672 CCATGG
TaqI 458 TCGA T^CGA
833 TCGA
TspRI 428 CACTG CA[GC]TG[ACGT][ACGT]^
858 CACTG
TthHB8I 458 TCGA T^CGA
833 TCGA
XhoII 41 AGATCC [GA]^GATC[CT]
518 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PvuII
SapI Sau3AI Sau96I ScrFI TseI
Tsp45I