Restriction map for
pF1KB6386
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI BstEII BstPI Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfaNI SfiI SfoI SgrAI SmaI
SmlI SpeI SphI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 657 GTATAC GT^[AC][GT]AC
AccII 445 CGCG CG^CG
AcsI 825 GAATTT [GA]^AATT[CT]
AfaI 421 GTAC GT^AC
Alw44I 696 GTGCAC G^TGCAC
AlwNI 449 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 128 AGCGCT AGC^GCT
ApaI 437 GGGCCC GGGCC^C
ApaLI 696 GTGCAC G^TGCAC
ApoI 825 GAATTT [GA]^AATT[CT]
Asp700I 822 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 778 GGCACC G^G[CT][GA]CC
BbrPI 40 CACGTG CAC^GTG
BbsI 754 GAAGAC GAAGAC(2/6)
BcgI 698 GCACTCATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 754 GAAGAC GAAGAC(2/6)
BsaI 408 GGTCTC GGTCTC(1/5)
BsaWI 428 ACCGGA [AT]^CCGG[AT]
Bsp1407I 420 TGTACA T^GTACA
BspMI 457 GCAGGT ACCTGC(4/8)
BsrDI 491 GCAATG GCAATG(2/0)
BsrFI 137 ACCGGC [GA]^CCGG[CT]
BsrGI 420 TGTACA T^GTACA
Bst1107I 657 GTATAC GTA^TAC
BstBI 706 TTCGAA TT^CGAA
BstF5I 692 GGATG GGATG(2/0)
BstUI 445 CGCG CG^CG
BstXI 155 CCAACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 657 GTATAC GTA^TAC
Cfr10I 137 ACCGGC [GA]^CCGG[CT]
DsaI 161 CCATGG C^C[GA][CT]GG
EaeI 98 TGGCCG [CT]^GGCC[GA]
Eco47III 128 AGCGCT AGC^GCT
EcoT14I 161 CCATGG C^C[AT][AT]GG
FokI 692 GGATG GGATG(9/13)
HaeII 128 AGCGCT [GA]GCGC^[CT]
HgaI 7 GCGTC GACGC(5/10)
MseI 13 TTAA T^TAA
MvnI 445 CGCG CG^CG
NcoI 161 CCATGG C^CATGG
NheI 615 GCTAGC G^CTAGC
NspV 706 TTCGAA TT^CGAA
PleI 87 GACTC GAGTC(4/5)
PmaCI 40 CACGTG CAC^GTG
PmlI 40 CACGTG CAC^GTG
PpuMI 743 AGGACCT [GA]G^G[AT]CC[CT]
PstI 455 CTGCAG CTGCA^G
PvuII 532 CAGCTG CAG^CTG
RsaI 421 GTAC GT^AC
SacI 293 GAGCTC GAGCT^C
SapI 709 GAAGAGC GCTCTTC(1/4)
SbfI 454 CCTGCAGG CCTGCA^GG
SfcI 455 CTGCAG C^T[GA][CT]AG
SfuI 706 TTCGAA TT^CGAA
SnaBI 809 TACGTA TAC^GTA
Sse8387I 454 CCTGCAGG CCTGCA^GG
SspBI 420 TGTACA T^GTACA
StyI 161 CCATGG C^C[AT][AT]GG
TaqI 707 TCGA T^CGA
Tru9I 13 TTAA T^TAA
Tsp509I 826 AATT ^AATT
TthHB8I 707 TCGA T^CGA
XmnI 822 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 168 GGACC G^G[AT]CC
744 GGACC
BanII 293 GAGCTC G[GA]GC[CT]^C
437 GGGCCC
BcnI 62 CCCGG CC^[GC]GG
399 CCGGG
BfaI 616 CTAG C^TAG
775 CTAG
BsaJI 61 CCCCGG C^C[ACGT][ACGT]GG
161 CCATGG
BseRI 199 GAGGAG GAGGAG(10/8)
790 GAGGAG
BsmFI 768 GGGAC GGGAC(10/14)
605 GTCCC
BstAPI 448 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
619 GCAGAATATGC
BstNI 867 CCAGG CC^[AT]GG
895 CCTGG
BstYI 395 AGATCC [GA]^GATC[CT]
855 AGATCC
EarI 553 CTCTTC CTCTTC(1/4)
709 GAAGAG
EcoRII 867 CCAGG ^CC[AT]GG
895 CCTGG
HphI 402 GGTGA GGTGA(8/7)
358 TCACC
Ksp632I 553 CTCTTC CTCTTC(1/4)
709 GAAGAG
MaeI 616 CTAG C^TAG
775 CTAG
MflI 395 AGATCC [GA]^GATC[CT]
855 AGATCC
MspA1I 361 CCGCTG C[AC]G^C[GT]G
532 CAGCTG
MvaI 867 CCAGG CC^[AT]GG
895 CCTGG
NciI 62 CCCGG CC^[GC]GG
399 CCGGG
TfiI 432 GATTC G^A[AT]TC
596 GAATC
TspRI 719 CACTG CA[GC]TG[ACGT][ACGT]^
872 CAGTG
XhoII 395 AGATCC [GA]^GATC[CT]
855 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BmyI BpmI BsaAI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MspI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I