Restriction map for
pF1KB6731
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# NO CUT ENZYMES ( 199 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaI HpaII HphI KasI
KpnI KspI MaeI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspI MunI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 229 GTCGAC GT^[AC][GT]AC
AciI 253 GCGG CCGC(-3/-1)
AflIII 158 ACATGT A^C[GA][CT]GT
AluI 212 AGCT AG^CT
AlwI 96 GGATC GGATC(4/5)
AlwNI 137 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 174 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 198 GGTCC G^G[AT]CC
BanII 189 GGGCTC G[GA]GC[CT]^C
BmyI 189 GGGCTC G[AGT]GC[ACT]^C
BsaAI 205 TACGTG [CT]AC^GT[GA]
BsaBI 97 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 208 GTGCAG GTGCAG(16/14)
BsiYI 87 CCATTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 87 CCATTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 24 GTCCC GGGAC(10/14)
Bsp1286I 189 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 158 ACATGT A^CATGT
Cac8I 216 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 198 GGTCC G^G[ACGT]CC
DpnII 97 GATC ^GATC
DrdI 124 GACATCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4 CTCTTC CTCTTC(1/4)
HincII 229 GTCGAC GT[CT]^[GA]AC
HindII 229 GTCGAC GT[CT]^[GA]AC
Ksp632I 4 CTCTTC CTCTTC(1/4)
MaeII 206 ACGT A^CGT
MaeIII 133 GTCAC ^GT[ACGT]AC
MamI 97 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 97 GATC ^GATC
MspA1I 211 CAGCTG C[AC]G^C[GT]G
NdeII 97 GATC ^GATC
NlaIII 159 CATG CATG^
NlaIV 79 GGAACC GG[ACGT]^[ACGT]CC
NspI 158 ACATGT [GA]CATG^[CT]
PflMI 87 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 271 CTGCAG CTGCA^G
PvuII 211 CAGCTG CAG^CTG
SalI 229 GTCGAC G^TCGAC
SapI 3 GCTCTTC GCTCTTC(1/4)
Sau3AI 97 GATC ^GATC
Sau96I 198 GGTCC G^G[ACGT]CC
TaqI 230 TCGA T^CGA
Tsp45I 133 GTCAC ^GT[GC]AC
TthHB8I 230 TCGA T^CGA
Van91I 87 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 174 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 5 enzymes) #
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BstF5I 39 GGATG GGATG(2/0)
249 GGATG
FokI 39 GGATG GGATG(9/13)
249 GGATG
MboII 171 GAAGA GAAGA(8/7)
5 TCTTC
SfaNI 250 GATGC GCATC(5/9)
71 GCATC
SfcI 244 CTACAG C^T[GA][CT]AG
271 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
BbvI DdeI Fnu4HI ItaI MnlI
TseI TspRI