Restriction map for
pF1KE0506
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbsI BciVI BclI BfrI BglI
BglII BlnI BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwI 399 GATCC GGATC(4/5)
AocI 142 CCTGAGG CC^T[ACGT]AGG
ApaI 505 GGGCCC GGGCC^C
AspHI 319 GAGCAC G[AT]GC[AT]^C
AviII 797 TGCGCA TGC^GCA
BanI 921 GGTGCC G^G[CT][GA]CC
BbrPI 325 CACGTG CAC^GTG
BbvCI 214 GCTGAGG CCTCAGC(-5/-2)
BcgI 941 GCAGCGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 36 GCTGAGC GC^T[ACGT]AGC
BpmI 958 CTCCAG CTGGAG(16/14)
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
BsaAI 325 CACGTG [CT]AC^GT[GA]
BsiHKAI 319 GAGCAC G[AT]GC[AT]^C
BspMI 853 GCAGGT ACCTGC(4/8)
BsrBI 76 CCGCTC CCGCTC(-3/-3)
BsrDI 479 GCAATG GCAATG(2/0)
BsrFI 110 GCCGGT [GA]^CCGG[CT]
BstEII 279 GGTCACC G^GT[ACGT]ACC
BstPI 279 GGTCACC G^GT[ACGT]ACC
Bsu36I 142 CCTGAGG CC^T[ACGT]AGG
CelII 36 GCTGAGC GC^T[ACGT]AGC
Cfr10I 110 GCCGGT [GA]^CCGG[CT]
DraI 178 TTTAAA TTT^AAA
EaeI 314 TGGCCG [CT]^GGCC[GA]
Eco81I 142 CCTGAGG CC^T[ACGT]AGG
EcoNI 693 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 279 GGTCACC G^GT[ACGT]ACC
EcoT14I 635 CCAAGG C^C[AT][AT]GG
FspI 797 TGCGCA TGC^GCA
HinfI 472 GATTC G^A[ACGT]TC
MseI 179 TTAA T^TAA
PmaCI 325 CACGTG CAC^GTG
PmlI 325 CACGTG CAC^GTG
SapI 90 GCTCTTC GCTCTTC(1/4)
StuI 975 AGGCCT AGG^CCT
StyI 635 CCAAGG C^C[AT][AT]GG
TfiI 472 GATTC G^A[AT]TC
Tru9I 179 TTAA T^TAA
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 306 CGCG CG^CG
433 CGCG
AflIII 239 ACGTGT A^C[GA][CT]GT
326 ACGTGT
AlwNI 382 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
811 CAGCCCCTG
Asp700I 543 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
738 GAAAAAGTTC
AvaI 774 CTCGGG C^[CT]CG[GA]G
925 CCCGAG
BanII 505 GGGCCC G[GA]GC[CT]^C
534 GAGCCC
BcnI 24 CCGGG CC^[GC]GG
52 CCCGG
BfaI 16 CTAG C^TAG
650 CTAG
BsaI 136 GAGACC GGTCTC(1/5)
55 GGTCTC
BseRI 771 CTCCTC GAGGAG(10/8)
169 GAGGAG
BsgI 7 CTGCAC GTGCAG(16/14)
265 GTGCAG
BsmBI 245 CGTCTC CGTCTC(1/5)
574 GAGACG
BsmFI 50 GTCCC GGGAC(10/14)
857 GTCCC
BsoBI 774 CTCGGG C^[CT]CG[GA]G
925 CCCGAG
BstAPI 107 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
844 GCACAGATTGC
BstUI 306 CGCG CG^CG
433 CGCG
BstXI 221 CCACATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
713 CCATTGTCATGG
Cac8I 267 GCAGGC GC[ACGT]^[ACGT]GC
408 GCTGGC
CfoI 307 GCGC GCG^C
798 GCGC
DpnII 288 GATC ^GATC
399 GATC
DraIII 22 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
322 CACCACGTG
DsaI 392 CCGTGG C^C[GA][CT]GG
515 CCACGG
EarI 91 CTCTTC CTCTTC(1/4)
613 CTCTTC
HhaI 307 GCGC GCG^C
798 GCGC
HinP1I 307 GCGC G^CGC
798 GCGC
HphI 396 GGTGA GGTGA(8/7)
281 TCACC
Ksp632I 91 CTCTTC CTCTTC(1/4)
613 CTCTTC
MaeI 16 CTAG C^TAG
650 CTAG
MboI 288 GATC ^GATC
399 GATC
MvnI 306 CGCG CG^CG
433 CGCG
NciI 24 CCGGG CC^[GC]GG
52 CCCGG
NdeII 288 GATC ^GATC
399 GATC
PflMI 402 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
912 CCACGCCCTGG
Sau3AI 288 GATC ^GATC
399 GATC
Van91I 402 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
912 CCACGCCCTGG
XmnI 543 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
738 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HpaII ItaI MaeII MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MwoI NlaIII NlaIV PpuMI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
TspRI TthHB8I