Restriction map for
pF1KA0449
##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflIII AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
BbrPI BciVI BsaAI BsaWI BseAI
BsiWI Bsp1407I BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRI EcoT22I HpaI KpnI KspI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3639 GACGTC GACGT^C
AflII 2203 CTTAAG C^TTAAG
Aor51HI 3792 AGCGCT AGC^GCT
AvrII 3616 CCTAGG C^CTAGG
BamHI 41 GGATCC G^GATCC
BbeI 2468 GGCGCC GGCGC^C
BcgI 206 GCAAGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 917 TGATCA T^GATCA
BfrI 2203 CTTAAG C^TTAAG
BlnI 3616 CCTAGG C^CTAGG
BsaBI 1926 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1333 ATCGAT AT^CGAT
BstEII 4341 GGTCACC G^GT[ACGT]ACC
BstPI 4341 GGTCACC G^GT[ACGT]ACC
ClaI 1333 ATCGAT AT^CGAT
DraIII 808 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3792 AGCGCT AGC^GCT
EcoO65I 4341 GGTCACC G^GT[ACGT]ACC
EcoRV 3472 GATATC GAT^ATC
FbaI 917 TGATCA T^GATCA
FseI 3 GGCCGGCC GGCCGG^CC
HincII 2800 GTCAAC GT[CT]^[GA]AC
HindII 2800 GTCAAC GT[CT]^[GA]AC
HindIII 1471 AAGCTT A^AGCTT
KasI 2468 GGCGCC G^GCGCC
MamI 1926 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2468 GGCGCC GG^CGCC
NheI 1389 GCTAGC G^CTAGC
NspI 710 GCATGT [GA]CATG^[CT]
PshAI 2793 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2856 CCTGCAGG CCTGCA^GG
ScaI 4025 AGTACT AGT^ACT
SexAI 728 ACCTGGT A^CC[AT]GGT
SfoI 2468 GGCGCC GGC^GCC
Sse8387I 2856 CCTGCAGG CCTGCA^GG
StuI 137 AGGCCT AGG^CCT
Tsp509I 4798 AATT ^AATT
XbaI 1476 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 926 CCTTAGG CC^T[ACGT]AGG
2433 CCTGAGG
AviII 707 TGCGCA TGC^GCA
4587 TGCGCA
BglII 185 AGATCT A^GATCT
3326 AGATCT
BlpI 4278 GCTTAGC GC^T[ACGT]AGC
4641 GCTCAGC
Bpu1102I 4278 GCTTAGC GC^T[ACGT]AGC
4641 GCTCAGC
BsiEI 2055 CGACCG CG[GA][CT]^CG
3669 CGACCG
BsmI 1269 GAATGC GAATGC(1/-1)
4106 GCATTC
BspHI 1025 TCATGA T^CATGA
1364 TCATGA
BssSI 529 CACGAG CACGAG(-5/-1)
4369 CTCGTG
BstAPI 2163 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2500 GCAGGGCGTGC
Bsu36I 926 CCTTAGG CC^T[ACGT]AGG
2433 CCTGAGG
CelII 4278 GCTTAGC GC^T[ACGT]AGC
4641 GCTCAGC
Eco81I 926 CCTTAGG CC^T[ACGT]AGG
2433 CCTGAGG
FspI 707 TGCGCA TGC^GCA
4587 TGCGCA
MseI 2204 TTAA T^TAA
4400 TTAA
PaeR7I 774 CTCGAG C^TCGAG
3626 CTCGAG
PflMI 435 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2634 CCACTTCTTGG
RcaI 1025 TCATGA T^CATGA
1364 TCATGA
SapI 1680 GAAGAGC GCTCTTC(1/4)
1774 GAAGAGC
SfiI 2271 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4312 GGCCACATCGGCC
Tru9I 2204 TTAA T^TAA
4400 TTAA
Van91I 435 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2634 CCACTTCTTGG
XhoI 774 CTCGAG C^TCGAG
3626 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI Tth111I TthHB8I XcmI
XhoII XmaCI XmaI