Restriction map for
pF1KA2022
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII BalI BbeI BbrPI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI Psp1406I
PvuI SacI SacII SalI ScaI
SfiI SfoI SfuI SnaBI SphI
SspBI Tth111I XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AgeI 150 ACCGGT A^CCGGT
ApaI 1058 GGGCCC GGGCC^C
AvrII 612 CCTAGG C^CTAGG
BbvCI 32 CCTCAGC CCTCAGC(-5/-2)
BclI 1971 TGATCA T^GATCA
BglI 1735 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 612 CCTAGG C^CTAGG
BsaBI 938 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 288 GAATGC GAATGC(1/-1)
BspLU11I 881 ACATGT A^CATGT
BsrFI 150 ACCGGT [GA]^CCGG[CT]
BstXI 3786 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 150 ACCGGT [GA]^CCGG[CT]
CpoI 4320 CGGACCG CG^G[AT]CCG
EaeI 3723 TGGCCG [CT]^GGCC[GA]
EcoNI 475 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2695 GAATTC G^AATTC
EcoRV 3061 GATATC GAT^ATC
FbaI 1971 TGATCA T^GATCA
HgaI 1019 GCGTC GACGC(5/10)
MaeII 526 ACGT A^CGT
MamI 938 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2225 CAATTG C^AATTG
MspA1I 592 CAGCTG C[AC]G^C[GT]G
MunI 2225 CAATTG C^AATTG
PinAI 150 ACCGGT A^CCGGT
PshAI 1372 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 592 CAGCTG CAG^CTG
RsrII 4320 CGGACCG CG^G[AT]CCG
SbfI 382 CCTGCAGG CCTGCA^GG
SexAI 1839 ACCAGGT A^CC[AT]GGT
SgrAI 149 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2430 CCCGGG CCC^GGG
SpeI 2482 ACTAGT A^CTAGT
Sse8387I 382 CCTGCAGG CCTGCA^GG
SspI 4104 AATATT AAT^ATT
SwaI 2069 ATTTAAAT ATTT^AAAT
XbaI 120 TCTAGA T^CTAGA
XmaCI 2430 CCCGGG C^CCGGG
XmaI 2430 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1261 CTTAAG C^TTAAG
3024 CTTAAG
AflIII 526 ACGTGT A^C[GA][CT]GT
881 ACATGT
AlwNI 533 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2084 CAGGCCCTG
AvaI 1391 CTCGGG C^[CT]CG[GA]G
2430 CCCGGG
BamHI 684 GGATCC G^GATCC
4047 GGATCC
BcgI 261 CGAACATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
420 GCAGCCAAGTCG
BciVI 177 GTATCC GTATCC(6/5)
3782 GTATCC
BcnI 2430 CCCGG CC^[GC]GG
2431 CCGGG
BfrI 1261 CTTAAG C^TTAAG
3024 CTTAAG
BglII 1340 AGATCT A^GATCT
2918 AGATCT
BsaI 166 GAGACC GGTCTC(1/5)
187 GGTCTC
BsaWI 150 ACCGGT [AT]^CCGG[AT]
3219 ACCGGA
BseRI 1413 CTCCTC GAGGAG(10/8)
1416 CTCCTC
BsoBI 1391 CTCGGG C^[CT]CG[GA]G
2430 CCCGGG
BspHI 2328 TCATGA T^CATGA
3998 TCATGA
BstAPI 2335 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2825 GCAGTAATTGC
FauI 228 CCCGC CCCGC(4/6)
3647 CCCGC
NciI 2430 CCCGG CC^[GC]GG
2431 CCGGG
NdeI 1346 CATATG CA^TATG
3415 CATATG
PflMI 90 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1234 CCATGCAGTGG
RcaI 2328 TCATGA T^CATGA
3998 TCATGA
SapI 2375 GCTCTTC GCTCTTC(1/4)
3569 GCTCTTC
StuI 321 AGGCCT AGG^CCT
2007 AGGCCT
Van91I 90 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1234 CCATGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI