Restriction map for
pF1KE1068
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AlwI AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BglI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
MamI MluI MroI MslI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI StuI SwaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 36 CGCG CG^CG
AfaI 1625 GTAC GT^AC
Aor51HI 1586 AGCGCT AGC^GCT
ApaI 15 GGGCCC GGGCC^C
Asp700I 445 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1634 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 326 TGGCCA TGG^CCA
BbvCI 1582 CCTCAGC CCTCAGC(-5/-2)
BciVI 577 GTATCC GTATCC(6/5)
BclI 428 TGATCA T^GATCA
BglII 1082 AGATCT A^GATCT
BlpI 373 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 373 GCTGAGC GC^T[ACGT]AGC
BsaI 793 GAGACC GGTCTC(1/5)
BseRI 1696 GAGGAG GAGGAG(10/8)
BspHI 945 TCATGA T^CATGA
BsrDI 303 CATTGC GCAATG(2/0)
Bst1107I 82 GTATAC GTA^TAC
BstAPI 1104 GCAACTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 36 CGCG CG^CG
BstYI 1082 AGATCT [GA]^GATC[CT]
BstZ17I 82 GTATAC GTA^TAC
CelII 373 GCTGAGC GC^T[ACGT]AGC
EaeI 326 TGGCCA [CT]^GGCC[GA]
Eco47III 1586 AGCGCT AGC^GCT
EcoRI 237 GAATTC G^AATTC
EcoT14I 194 CCATGG C^C[AT][AT]GG
FbaI 428 TGATCA T^GATCA
HindIII 1180 AAGCTT A^AGCTT
KspI 35 CCGCGG CCGC^GG
MfeI 1227 CAATTG C^AATTG
MflI 1082 AGATCT [GA]^GATC[CT]
MluNI 326 TGGCCA TGG^CCA
MscI 326 TGGCCA TGG^CCA
MspA1I 35 CCGCGG C[AC]G^C[GT]G
MunI 1227 CAATTG C^AATTG
MvnI 36 CGCG CG^CG
NcoI 194 CCATGG C^CATGG
NdeI 1354 CATATG CA^TATG
PacI 313 TTAATTAA TTAAT^TAA
PflFI 1634 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1303 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 677 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 945 TCATGA T^CATGA
RsaI 1625 GTAC GT^AC
SacII 35 CCGCGG CCGC^GG
SapI 448 GCTCTTC GCTCTTC(1/4)
SbfI 242 CCTGCAGG CCTGCA^GG
Sse8387I 242 CCTGCAGG CCTGCA^GG
SspI 500 AATATT AAT^ATT
StyI 194 CCATGG C^C[AT][AT]GG
TaqI 1215 TCGA T^CGA
Tth111I 1634 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1215 TCGA T^CGA
Van91I 1303 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 774 TCTAGA T^CTAGA
XhoII 1082 AGATCT [GA]^GATC[CT]
XmnI 445 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 95 GAATTT [GA]^AATT[CT]
237 GAATTC
AflII 160 CTTAAG C^TTAAG
892 CTTAAG
AflIII 802 ACATGT A^C[GA][CT]GT
1396 ACATGT
ApoI 95 GAATTT [GA]^AATT[CT]
237 GAATTC
BanII 15 GGGCCC G[GA]GC[CT]^C
1560 GAGCCC
BbsI 289 GAAGAC GAAGAC(2/6)
1332 GAAGAC
BfrI 160 CTTAAG C^TTAAG
892 CTTAAG
BpuAI 289 GAAGAC GAAGAC(2/6)
1332 GAAGAC
BsmI 469 GAATGC GAATGC(1/-1)
560 GAATGC
BspLU11I 802 ACATGT A^CATGT
1396 ACATGT
BspMI 831 ACCTGC ACCTGC(4/8)
573 GCAGGT
DraII 15 GGGCCCC [GA]G^G[ACGT]CC[CT]
1609 GGGGCCT
DsaI 35 CCGCGG C^C[GA][CT]GG
194 CCATGG
EarI 449 CTCTTC CTCTTC(1/4)
1687 GAAGAG
EcoO109I 15 GGGCCCC [GA]G^G[ACGT]CC[CT]
1609 GGGGCCT
FauI 23 CCCGC CCCGC(4/6)
13 GCGGG
HaeII 1145 AGCGCC [GA]GCGC^[CT]
1586 AGCGCT
HgaI 299 GACGC GACGC(5/10)
1441 GCGTC
Ksp632I 449 CTCTTC CTCTTC(1/4)
1687 GAAGAG
MaeII 8 ACGT A^CGT
86 ACGT
NspI 802 ACATGT [GA]CATG^[CT]
1396 ACATGT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccI AciI AluI AlwNI BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI